Stress-induced neutral lipid biosynthesis in microalgae — Molecular, cellular and physiological insights

Zienkiewicz, Krzysztof; Du, Zhi-Yan; Ma, Wei; Vollheyde, Katharina; Benning, Christoph

doi:10.1016/j.bbalip.2016.02.008

Cited by 225Lipid metabolism and biosynthesisAlgal biology and biofuel productionMicrobial Metabolic Engineering and Bioproduction

Stress-induced neutral lipid biosynthesis in microalgae — Molecular, cellular and physiological insights

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Zienkiewicz, Krzysztof, Du, Zhi-Yan, Ma, Wei, Vollheyde, Katharina, Benning, Christoph·2016·Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

DOI 10.1016/j.bbalip.2016.02.008

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Abstract

Photosynthetic microalgae have promise as biofuel feedstock. Under certain conditions, they produce substantial amounts of neutral lipids, mainly in the form of triacylglycerols (TAGs), which can be converted to fuels. Much of our current knowledge on the genetic and molecular basis of algal neutral lipid metabolism derives mainly from studies of plants, i.e. seed tissues, and to a lesser extent from direct studies of algal lipid metabolism. Thus, the knowledge of TAG synthesis and the cellular trafficking of TAG precursors in algal cells is to a large extent based on genome predictions, and most aspects of TAG metabolism have yet to be experimentally verified. The biofuel prospects of microalgae have raised the interest in mechanistic studies of algal TAG biosynthesis in recent years and resulted in an increasing number of publications on lipid metabolism in microalgae. In this review we summarize the current findings on genetic, molecular and physiological studies of TAG accumulation in microalgae. Special emphasis is on the functional analysis of key genes involved in TAG synthesis, molecular mechanisms of regulation of TAG biosynthesis, as well as on possible mechanisms of lipid droplet formation in microalgal cells. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner.

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Nearly 80% of the TAGs are saturated and monounsaturated fatty acids.
“Studies have shown that nearly 80% of the TAGs are saturated (SFA) and monounsaturated fatty acids (MUFA)[3,52,61,62], and their vibration can be attributed to the carbonyl stretches of esters in the fatty acids at ~1740 cm -1 .”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [62]
The two species M. contortum and Chlamydomonas sp. have produced more neutral lipids (TAGs) with nutritional depletion.
“This indicates that the two species M.contortum and Chlamydomonas sp. have produced more neutral lipids (TAGs) with nutritional depletion [9,20,61 -63] and it is a positive indication that these microalgae species can be tapped for biodiesel production.”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [62]

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Changes in the cell composition of the marine microalga, Nannochloropsis gaditana, during a light:dark cycle
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Rapid Induction of Lipid Droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by Brefeldin A
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Identification of a triacylglycerol lipase in the diatom Phaeodactylum tricornutum
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EFFECT OF INCUBATION TEMPERATURE ON THE MACROMOLECULAR AND LIPID CONTENT OF THE PHYTOFLAGELLATE OCHROMONAS DANICA1
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Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator
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The lipid biochemistry of eukaryotic algae
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Plant Lipid Droplets and Their Associated Proteins: Potential for Rapid Advances
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Current trends to comprehend lipid metabolism in diatoms
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Reactive Oxygen Species-Mediated Cellular Stress Response and Lipid Accumulation in Oleaginous Microorganisms: The State of the Art and Future Perspectives
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The Growth Factors Involved in Microalgae Cultivation for Biofuel Production: A Review
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Self-citation

Authors: Zienkiewicz, K., Du, Z.-Y., Ma, W., Vollheyde, K., & Benning, C.
Affiliations: University of Göttingen (DE)
Michigan State University (US)
Great Lakes Bioenergy Research Center (US)
ORCID: Zienkiewicz, Krzysztof
Du, Zhi-Yan
Ma, Wei
Vollheyde, Katharina
Venue: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Publisher: Elsevier BV
Published: 2016-09
Volume: 1861
Issue: 9
Pages: 1269-1281
ISSN: 1388-1981 (ISSN-L)
DOI: 10.1016/j.bbalip.2016.02.008
OpenAlex: W2258753467
Cited by: 225
References: 148
Citations by year: 2026: 15 · 2025: 12 · 2024: 23 · 2023: 17 · 2022: 10 · 2021: 30 · 2020: 38 · 2019: 24 · 2018: 21 · 2017: 30 · 2016: 5
FWCI: 3.32
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (am, 327 d)
doi.org/10.15223/policy-017 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-012 (stm-asf)
doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-004 (stm-asf)
Locations: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (acceptedVersion)
PubMed (publishedVersion)
OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
GRO (submittedVersion)
Funders: Center for Advanced Biofuels Systems (DE-SC0001295)
U.S. Department of Energy, Office of Basic Energy Sciences (DE-SC0001295)
U.S Department of Energy, Office of Basic Energy Sciences (DE-FG02-98ER20305)
US National Science Foundation (MCB-0741395, MCB-1515169)
US Department of Energy-Great Lakes Bioenergy Research Center Cooperative Agreement (DE-FC02-07ER64494)
US Air Force Office of Scientific Research (FA9550-11-1-0264)
Michigan State University AgBioResearch
Marie Curie International Outgoing Fellowship within the EU 7th Framework Program AlgaeOilSynth (627266, FP7-PEOPLE-2013-IOF)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: microalgae, triglyceride synthesis, stress response, lipid droplet formation, lipid metabolism, biofuel feedstock
MeSH terms: Fatty Acids, Lipids, Photosynthesis, Triglycerides, Genome, Plant, Lipid Metabolism, Biofuels, Microalgae

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Stress-induced neutral lipid biosynthesis in microalgae — Molecular, cellular and physiological insights

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