Ovarian cancer cell lines derived from non-serous carcinomas migrate and invade more aggressively than those derived from high-grade serous carcinomas
Open accessAbstract
The term ovarian cancer describes a heterogeneous group of tumours that grow in the ovary but are not necessarily of ovarian origin. Recent genomic analysis has shown that many of the most commonly used ovarian cancer cell lines have been mischaracterised, leading to erroneous conclusions and a gap in the translation of laboratory research into novel treatments for patients. Here, we use 10 epithelial ovarian cancer cell lines to investigate 2D migration, cell cycle parameters and 3D invasion behaviour into different substrates and find significant differences between the behaviours of cell lines from different origins. Cell lines derived from non-serous carcinomas migrated more quickly and were more likely to invade into Matrigel and collagen I substrates than cell lines derived from high-grade serous carcinomas. However not all cell lines derived from non-serous carcinomas exhibited similar invasive behaviour. These findings may reflect differences in the behaviour of the primary tumour types from which the cell lines were derived, given that high-grade serous carcinomas typically expand and spread over peritoneal surfaces. These results provide the basis of an in vitro model for identifying differences between ovarian cancer tumour types.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Unstimulated OVCAR-3 cells could remain largely in a proliferation-quiescent state within a 3D matrix.
“it has been reported that unstimulated OVCAR-3 could remain largely in a proliferationquiescent state within a 3D matrix [64, 66].”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.21492 · Cited by 84,109 · see citing works
Ovarian cancer statistics, 2018
Torre, L. A., Trabert, B., DeSantis, C. E., Miller, K. D., Samimi, G., Runowicz, C. D., Gaudet, M. M., Jemal, A., & Siegel, R. L. (2018) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.21456 · Cited by 3,492 · see citing works
Tumour-cell invasion and migration: diversity and escape mechanisms
Friedl, P., & Wolf, K. (2003) · Nature reviews. Cancer · doi:10.1038/nrc1075 · Cited by 3,225 · see citing works
Ovarian Cancer Development and Metastasis
Lengyel, E. R. (2010) · American Journal Of Pathology · doi:10.2353/ajpath.2010.100105 · Cited by 1,679 · see citing works
Eskenazi, B., & Warner, M. L. (1997) · Obstetrics and Gynecology Clinics of North America · doi:10.1016/s0889-8545(05)70302-8 · Cited by 1,617 · see citing works
One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599 · see citing works
Rethinking ovarian cancer: recommendations for improving outcomes
Vaughan, S., Coward, J. I. G., Bast, R. C., Berchuck, A., Berek, J. S., Brenton, J. D., Coukos, G., Crum, C. P., Drapkin, R., Etemadmoghadam, D., et al. (2011) · Nature reviews. Cancer · doi:10.1038/nrc3144 · Cited by 1,470 · see citing works
Evaluating cell lines as tumour models by comparison of genomic profiles
Domcke, S., Sinha, R., Levine, D. A., Sander, C., & Schultz, N. D. (2013) · Nature Communications · doi:10.1038/ncomms3126 · Cited by 1,426 · see citing works
Fogh, J., & Trempe, G. (1975) · Springer eBooks · doi:10.1007/978-1-4757-1647-4_5 · Cited by 1,008 · see citing works
Absence of HeLa Cell Contamination in 169 Cell Lines Derived From Human Tumors2
Fogh, J., Wright, W. C., & Loveless, J. D. (1977) · JNCI: Journal of the National Cancer Institute · doi:10.1093/jnci/58.2.209 · Cited by 663 · see citing works
Burstein, H. J., Krilov, L., Aragon‐Ching, J. B., Baxter, N. N., Chiorean, E. G., Chow, W. A., Groot, J. F. D., Devine, S. M., DuBois, S. G., El‐Deiry, W. S., et al. (2017) · Journal of Clinical Oncology · doi:10.1200/jco.2016.71.5292 · Cited by 601 · see citing works
Hamilton, T. C., Young, R. C., McKoy, W. M., Grotzinger, K. R., Green, J. A., Chu, E. W., Whang‐Peng, J., Rogan, A. M., Green, W. R., & Ozols, R. F. (1983) · PubMed · Cited by 539 · see citing works
Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells
Eva, A., Robbins, K. C., Andersen, P. R., Srinivasan, A., Tronick, S. R., Reddy, E. P., Ellmore, N., Galen, A. T., Lautenberger, J. A., Papas, T. S., et al. (1982) · Nature · doi:10.1038/295116a0 · Cited by 498 · see citing works
Ahmed, N., & Stenvers, K. L. (2013) · Frontiers in Oncology · doi:10.3389/fonc.2013.00256 · Cited by 482 · see citing works
Ovarian Cancer Cell Line Panel (OCCP): Clinical Importance of In Vitro Morphological Subtypes
Beaufort, C. M., Helmijr, J. C. A., Piskorz, A. M., Hoogstraat, M., Ruigrok-Ritstier, K., Besselink, N., Murtaza, M., van IJcken, W. F. J., Heine, A. A. J., Smid, M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103988 · Cited by 403 · see citing works
A cancer cell metalloprotease triad regulates the basement membrane transmigration programPreprint
Hotary, K. B., Li, X.-Y., Allen, E. D., Stevens, S. L., & Weiss, S. J. (2006) · Genes & Development · doi:10.1101/gad.1451806 · Cited by 382 · see citing works
Cancer Invasion: Patterns and Mechanisms
Krakhmal, N. V., Zavyalova, M. V., Denisov, E. V., Vtorushin, S. V., & Михайлович, П. В. (2015) · Acta Naturae · doi:10.32607/20758251-2015-7-2-17-28 · Cited by 373 · see citing works
Human cancer cell lines: fact and fantasy
Masters, J. R. W. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35043102 · Cited by 352 · see citing works
New insights into ovarian cancer pathology
Prat, J. (2012) · Annals of Oncology · doi:10.1093/annonc/mds300 · Cited by 319 · see citing works
The disparate origins of ovarian cancers: pathogenesis and prevention strategies
Karnezis, A. N., Cho, K. R., Gilks, C. B., Pearce, C. L., & Huntsman, D. D. (2016) · Nature reviews. Cancer · doi:10.1038/nrc.2016.113 · Cited by 294 · see citing works
Shaw, T. J., Senterman, M. K., Dawson, K. M., Crane, C. A., & Vanderhyden, B. C. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.08.013 · Cited by 278 · see citing works
Dizon, D. S., Krilov, L., Cohen, E. E. W., Gangadhar, T. C., Ganz, P. A., Hensing, T. A., Hunger, S. P., Krishnamurthi, S., Lassman, A. B., Markham, M. J., et al. (2016) · Journal of Clinical Oncology · doi:10.1200/jco.2015.65.8427 · Cited by 213 · see citing works
Metallothionein gene expression and resistance to cisplatin in human ovarian cancer
Schilder, R. J., HALL, L., Monks, A. P., Handel, L. M., Fornace, A. J., Ozols, R. F., Fojo, A. T., & Hamilton, T. C. (1990) · International Journal of Cancer · doi:10.1002/ijc.2910450306 · Cited by 185 · see citing works
LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells
Scott, R. W., Hooper, S., Crighton, D., Li, A., König, I., Munro, J., Trivier, E., Wickman, G., Morin, P., Croft, D. R., et al. (2010) · The Journal of Cell Biology · doi:10.1083/jcb.201002041 · Cited by 175 · see citing works
Self-citationvan den Berg‐Bakker, C. A. M., Hagemeijer, A., Franken‐Postma, E. M., Smit, V. T. H. B. M., Kuppen, P. J. K., Claasen, H. H. V. R., Cornelisse, C. J., & Schrier, P. I. (1993) · International Journal of Cancer · doi:10.1002/ijc.2910530415 · Cited by 140 · see citing works
Wolf, C. R., Hayward, I. P., Lawrie, S. S., BUCKTON, K. E., McIntyre, M. A., Adams, D. J., Lewis, A. D., Scott, A. R. R., & Smyth, J. F. (1987) · International Journal of Cancer · doi:10.1002/ijc.2910390607 · Cited by 135 · see citing works
The Extracellular Matrix in Epithelial Ovarian Cancer – A Piece of a Puzzle
Cho, A., Howell, V. M., & Colvin, E. K. (2015) · Frontiers in Oncology · doi:10.3389/fonc.2015.00245 · Cited by 132 · see citing works
Characterization of Ovarian Cancer Cell Lines as In Vivo Models for Preclinical Studies
Hernandez, L., Kim, M. K., Lyle, L. T., Bunch, K. P., House, C. D., Ning, F., Noonan, A. M., & Annunziata, C. M. (2016) · Gynecologic Oncology · doi:10.1016/j.ygyno.2016.05.028 · Cited by 121 · see citing works
Fos-transformation activates genes associated with invasion.
Hennigan, R. F., Hawker, K., & Ozanne, B. W. (1994) · PubMed · Cited by 120 · see citing works
Sundar, S. S., Neal, R. D., & Kehoe, S. T. (2015) · BMJ · doi:10.1136/bmj.h4443 · Cited by 120 · see citing works
Divide or Conquer: Cell Cycle Regulation of Invasive Behavior
Kohrman, A. Q., & Matus, D. Q. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.08.003 · Cited by 114 · see citing works
Sodek, K. L., Brown, T. J., & Ringuette, M. J. (2008) · BMC Cancer · doi:10.1186/1471-2407-8-223 · Cited by 111 · see citing works
Timpson, P., McGhee, E. J., Erami, Z., Nobis, M., Quinn, J. A., Edward, M., & Anderson, K. I. (2011) · Journal of Visualized Experiments · doi:10.3791/3089 · Cited by 101 · see citing works
Yano, S., Miwa, S., Mii, S., et al. (2014) · Cell Cycle · doi:10.4161/cc.27818 · Cited by 79 · see citing works
Wilson, A. P. (1984) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/72.3.513 · Cited by 72 · see citing works
The promise and challenge of ovarian cancer models.
Hasan, N., Öhman, A., & Dinulescu, D. M. (2015) · PubMed · doi:10.3978/j.issn.2218-676x.2015.01.02 · Cited by 46 · see citing works
Mutational heterogeneity in non-serous ovarian cancers
Teer, J. K., Yoder, S., Gjyshi, A., Nicosia, S. V., Zhang, C. H., & Monteiro, Á. N. A. (2017) · Scientific Reports · doi:10.1038/s41598-017-10432-9 · Cited by 46 · see citing works
Zhong, Y.-C., Zhang, T., Di, W. J., et al. (2013) · Journal of Gynecologic Oncology · doi:10.3802/jgo.2013.24.3.265 · Cited by 38 · see citing works
Mutant p53 stimulates cell invasion through an interaction with Rad21 in human ovarian cancer cells
Ahn, J., Kim, T. J., Lee, J. H., & Choi, J. (2017) · Scientific Reports · doi:10.1038/s41598-017-08880-4 · Cited by 37 · see citing works
Characterisation of seven human ovarian tumour cell lines
Wilson, A., Dent, M. F., Pejovic, T. B., Hubbold, L., & Radford, H. (1996) · British Journal of Cancer · doi:10.1038/bjc.1996.428 · Cited by 22 · see citing works
Emoto, M., Oshima, K., Ishiguro, M., et al. (1999) · Pathology - Research and Practice · doi:10.1016/s0344-0338(99)80040-5 · Cited by 11 · see citing works
AGED GARLIC EXTRACT SUPPRESSES LIPID PEROXIDATION INDUCED BY β-AMYLOID IN PC12 CELLS
Griffin, B., Selassie, M., & Gwebu, E. T. (2000) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1290/1071-2690(2000)036 · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Rosenblum, D., Gutkin, A., Kedmi, R., Ramishetti, S., Veiga, N., Jacobi, A. M., Schubert, M. S., Friedmann-Morvinski, D., Cohen, Z. R., Behlke, M. A., et al. (2020) · Science Advances · doi:10.1126/sciadv.abc9450 · Cited by 614 · see citing works
Surendran, V., Rutledge, D., Colmon, R., & Chandrasekaran, A. (2021) · Biofabrication · doi:10.1088/1758-5090/abe1cf · Cited by 99 · see citing works
Yee, C., Dickson, K.-A., Muntasir, M. N., Ma, Y., & Marsh, D. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.836984 · Cited by 66 · see citing works
Ibrahim, L. I., Hajal, C., Offeddu, G. S., Gillrie, M. R., & Kamm, R. D. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121728 · Cited by 53 · see citing works
- Authors
- Hallas-Potts, A., Dawson, J. C., & Herrington, C. S.
- Affiliations
- Edinburgh Cancer Research (GB)
- Corresponding author
- Herrington, C. Simon
- Venue
- Scientific Reports
- Published
- 2019-04-02
- Volume
- 9
- Issue
- 1
- Pages
- 5515-5515
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC6445084
- OpenAlex
- W2926272439
- Cited by
- 110
- References
- 42
- Citations by year
- 2026: 10 · 2025: 13 · 2024: 18 · 2023: 18 · 2022: 16 · 2021: 17 · 2020: 15 · 2019: 3
- FWCI
- 12.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
- Funders
- RCUK | Engineering and Physical Sciences Research Council (EP/L016559/1)
- RCUK | Medical Research Council (EP/L016559/1)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Keywords
- ovarian cancer cell lines, high-grade serous carcinoma, cell migration, cell cycle, Matrigel, type I collagen, tumor heterogeneity, peritoneal spread
- MeSH terms
- Carcinoma, Ovarian Epithelial, Cell Cycle, Cell Movement, Female, Humans, Models, Biological, Ovarian Neoplasms, Cystadenocarcinoma, Serous, Cell Culture Techniques, Cell Line, Tumor, Neoplasm Grading
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
