Absence of HeLa Cell Contamination in 169 Cell Lines Derived From Human Tumors2
Abstract
Numerous cell lines derived from human tumors are not HeLa contaminants. Of 192 lines established in this or other laboratories, 169 lines were found to be G6PD type B. Twenty-three lines were type A as HeLa; three of these were of Negroid origin. There is reasonable doubt that the remaining 20 lines will all be shown to be confounded with HeLa.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HeLa and its derivatives HeLa S3, AV3 and WISH contaminations are notorious problems and may be simply detected by either isoenzyme or STR profiling.
“HeLa [16] and its derivatives HeLa S3, AV3 [17] and WISH contaminations are notorious problems and may be simply detected by either isoenzyme [18] or STR profiling [19].”
From Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines (2017) · cites this paper as [16]
Cited by
Sorted by citations
Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization
Cailleau, R., Olivé, M., & Cruciger, Q. V. J. (1978) · In Vitro · doi:10.1007/bf02616120 · Cited by 679 · see citing works
Origin of the U87MG glioma cell line: Good news and bad news
Allen, M., Bjerke, M., Edlund, H., Nelander, S., & Westermark, B. (2016) · Science Translational Medicine · doi:10.1126/scitranslmed.aaf6853 · Cited by 466 · see citing works
Cross-contamination of cells in culture
Nelson-Rees, W. A., Daniels, D. W., & Flandermeyer, R. R. (1981) · Science · doi:10.1126/science.6451928 · Cited by 329 · see citing works
Characterization of Ovarian Cancer Cell Lines as In Vivo Models for Preclinical Studies
Hernandez, L., Kim, M. K., Lyle, L. T., Bunch, K. P., House, C. D., Ning, F., Noonan, A. M., & Annunziata, C. M. (2016) · Gynecologic Oncology · doi:10.1016/j.ygyno.2016.05.028 · Cited by 121 · see citing works
Hallas-Potts, A., Dawson, J. C., & Herrington, C. S. (2019) · Scientific Reports · doi:10.1038/s41598-019-41941-4 · Cited by 110 · see citing works
Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines
Huang, Y., Liu, Y., Zheng, C., & Shen, C. (2017) · PLOS ONE · doi:10.1371/journal.pone.0170384 · Cited by 105 · see citing works
Espinha, G., Osaki, J. H., Costa, E. T., & Forti, F. L. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/2696952 · Cited by 21 · see citing works
Gu, M., Yang, M., He, J., Xia, S., Zhang, Z., Wang, Y., Zheng, C., & Shen, C. (2022) · International Journal of Cancer · doi:10.1002/ijc.33789 · Cited by 11 · see citing works
- Authors
- Fogh, J., Wright, W. C., & Loveless, J. D.
- ORCID
- Wright, William C.
- Venue
- JNCI: Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1977-02
- Volume
- 58
- Issue
- 2
- Pages
- 209-214
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W2255703111
- Cited by
- 663
- References
- 0
- Citations by year
- 2026: 10 · 2025: 12 · 2024: 15 · 2023: 17 · 2022: 14 · 2021: 8 · 2020: 14 · 2019: 12 · 2018: 14 · 2017: 16 · 2016: 19 · 2015: 19 · 2014: 11 · 2013: 25 · 2012: 16
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- JNCI Journal of the National Cancer Institute (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- human cancer cell lines, glucose-6-phosphate dehydrogenase, cell line authentication, cell line contamination
- MeSH terms
- Cell Line, Glucosephosphate Dehydrogenase, HeLa Cells, Humans, Isoenzymes, Neoplasms, Phenotype, Black People, White People
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
