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Re-epithelialization and immune cell behaviour in an ex vivo human skin model

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Rakita, Ana, Nikolić, Nenad, Mildner, Michael, Matiasek, Johannes, Elbe-Bürger, Adelheid·2020·Scientific Reports
DOI 10.1038/s41598-019-56847-4
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Abstract

A large body of literature is available on wound healing in humans. Nonetheless, a standardized ex vivo wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14 + Ki67 + keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.

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Metadata

Authors
Rakita, A., Nikolić, N., Mildner, M., Matiasek, J., & Elbe-Bürger, A.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-01-08
Volume
10
Issue
1
Pages
1-1
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-56847-4
OpenAlex
W3004227146
Cited by
8,228
References
0
FWCI
765.15
Type
journal article
Language
en
Open access
gold
Licence
Funders
Medical Scientific Fund of the Mayor of the City of Vienna: 18045

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CoChat. (2026). Citation check: Re-epithelialization and immune cell behaviour in an ex vivo human skin model. https://app.cochat.ai/papers/10.1038/s41598-019-56847-4
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Rakita, A., Nikolić, N., Mildner, M., Matiasek, J., & Elbe-Bürger, A. (2020). Re-epithelialization and immune cell behaviour in an ex vivo human skin model. Scientific Reports. https://doi.org/10.1038/s41598-019-56847-4

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