Isolation and characterization of novel Bacillus strains with superior probiotic potential: comparative analysis and safety evaluation
Open accessAbstract
Despite the current use of some Bacillus spp. as probiotics, looking for and introducing new efficient and safe potential probiotic strains is one of the most important topics in both microbiology and food industry. This study aimed to isolate, identify, and evaluate the probiotic characteristics and safety of some Bacillus spp. from natural sources. Thirty-six spore-forming, Gram-positive, and catalase-positive Bacillus isolates were identified in 54 samples of soil, feces and dairy products. Bacterial identification was performed using 16S rDNA sequencing. To evaluate the probiotic potential of isolates, the resistance of bacterial cells to simulated gastrointestinal tract (GIT) conditions, the presence of enterotoxin genes, their susceptibility to antibiotics, antimicrobial and hemolytic activities and biochemical profiles were investigated. The results revealed that eight sporulating Bacillus spp. isolates fulfilled all tested probiotic criteria. They showed a high growth rate, non-hemolytic and lecithinase activity, and resistance to simulated GIT conditions. These strains exhibited broad-spectrum antibacterial activity against pathogenic bacteria. In addition, they did not exhibit antibacterial resistance to the 12 tested antibiotics. The results of this study suggest that these isolates can be considered as candidates for functional foods and as safe additives to improve diet quality.
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Safety studies have been mostly performed for the use of Bacillus probiotics as food additives in humans, but they are lacking when they are used as sanitizers in PBS.
“In addition, safety studies have been mostly performed for the use of Bacillus probiotics as food additives in humans, also recently [46], but they are lacking when they are used as sanitizers in PBS.”
From Profiling the resistome and virulome of Bacillus strains used for probiotic-based sanitation: a multicenter WGS analysis (2025) · cites this paper as [46]
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- Authors
- Golnari, M., Bahrami, N., Milanian, Z., Rabbani Khorasgani, M., Asadollahi, M. A., Shafiei, R., & Fatemi, S. S.-A.
- Affiliations
- University of Isfahan (IR)
- NourDanesh Institute of Higher Education (IR)
- National Institute of Genetic Engineering and Biotechnology (IR)
- Corresponding author
- Asadollahi, Mohammad Ali
- Venue
- Scientific Reports
- Published
- 2024-01-17
- Volume
- 14
- Issue
- 1
- Pages
- 1457-1457
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC10791968
- OpenAlex
- W4390953960
- Cited by
- 86
- References
- 61
- Citations by year
- 2026: 37 · 2025: 36 · 2024: 13
- FWCI
- 18.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ehyagaran Ghalb Sanat Asia (94/146295)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- Bacillus, probiotic potential, probiotic safety, gastrointestinal resistance, enterotoxin genes, antibiotic susceptibility, antibacterial activity, hemolytic activity, lecithinase activity, soil bacteria, fecal bacteria, dairy products, functional foods, 16S rRNA identification
- MeSH terms
- Anti-Bacterial Agents, Bacillus, Feces, Gastrointestinal Diseases, Humans, Probiotics
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