Identification and evaluation of in vitro probiotic attributes of novel and potential strains of lactic acid bacteria isolated from traditional dairy products of North-West Himalayas
Open accessAbstract
Background: Fermented dairy products of north-west Himalayas (Himachal Pradesh) are rich sources of potential and novel probiotic isolates which further can be explored for their commercial application.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Adhesion of microorganisms to epithelial cells is related to both hydrophobic properties and the ability to auto-aggregate.
“The adhesion of microorganisms to epithelial cells is related to both hydrophobic properties and the ability to auto aggregate, which influences cell surface interactions.”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [39]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Probiotics: an overview of beneficial effects
Ouwehand, A. C., Salminen, S., & Isolauri, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020620607611 · Cited by 1,045 · see citing works
Kos, B., Šušković, J., Vuković, S., Šimpraga, M., Frece, J., & Matošić, S. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01915.x · Cited by 880 · see citing works
Taxonomy and important features of probiotic microorganisms in food and nutrition
Holzapfel, W. H., Haberer, P., Geisen, R. M., Björkroth, J. K., & Schillinger, U. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.365s · Cited by 763 · cited 1× · see citing works
“There are many potential applications of protective cultures in various food systems and one of that is to impart them the special status of probiotics after their rigorous characterization [1].”
BackgroundAdhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum
Del Re, B., Sgorbati, B., Miglioli, M., & Palenzona, D. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.2000.00845.x · Cited by 731 · see citing works
The lactic acid bacteria: A literature survey
Carr, F. J., Chill, D., & Maida, N. (2002) · Critical Reviews in Microbiology · doi:10.1080/1040-840291046759 · Cited by 724 · cited 1× · see citing works
“fermented milk, sourdough, silage and alcoholic beverages, etc [3].”
BackgroundAdolfsson, O., Meydani, S. N., & Russell, R. M. (2004) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/80.2.245 · Cited by 531 · cited 1× · see citing works
“to generate a health benefi t for the consumer [4].”
BackgroundCholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut
Pereira, D. I. A., & Gibson, G. R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.9.4689-4693.2002 · Cited by 481 · cited 1× · see citing works
“The resistance of the strains to bile salt was performed according to Dora and Glenn (2002) [10].”
MethodProbiotic properties of human lactobacilli strains to be used in the gastrointestinal tract
Fernández, M. F., Boris, S., & Barbés, C. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01850.x · Cited by 441 · see citing works
Bao, Y., Zhang, Y., Zhang, Y., Liu, Y., Wang, S., Dong, X., Wang, Y., & Zhang, H. (2009) · Food Control · doi:10.1016/j.foodcont.2009.10.010 · Cited by 395 · cited 1× · see citing works
“Probiotics are able to achieve coaggregation with pathogenic microorganisms and in turn effectively inhibit and kill them by secreting antimicrobial compounds that act directly on the cells of pathogenic bacteria [32].”
BackgroundRoos, S., & Jonsson, H. (2002) · Microbiology · doi:10.1099/00221287-148-2-433 · Cited by 376 · see citing works
Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics
Mishra, V., & Prasad, D. N. (2005) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.10.047 · Cited by 269 · cited 1× · see citing works
“Bacterial adhesion to hydrocarbons was determined by the method of Mishra and Prasad (2005) [11].”
MethodRojas, M., Ascencio, F., & Conway, P. L. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.5.2330-2336.2002 · Cited by 197 · cited 1× · see citing works
“The initial interaction may be weak, followed by adhesion processes that are mediated by more specifi c mechanisms involving cell surface proteins and lipoteichoic acids [28,29].”
BackgroundMaeda, H., Xia Shuang, Z. H. U., Suzuki, S., Suzuki, K., & Kitamura, S. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf049617g · Cited by 171 · cited 1× · see citing works
“Fermented food products are regarded as signifi cant part of the functional foods market and their benefi cial effects are mainly associated with the maintenance of the healthy gut microbiota and an improvement of its resilience, as well as the modulation of lactose intolerance, bowel function and gastrointestinal com…”
BackgroundCollado, M. C., Meriluoto, J. A. O., & Salminen, S. (2007) · Food Research International · doi:10.1016/j.foodres.2006.11.007 · Cited by 142 · cited 1× · see citing works
“Indeed, the probiotic ability to adhere to the intestinal epithelium is regarded as a prerequisite to colonize the human GIT (gastrointestinal tract) for exerting benefi cial effects of probiotics such as the exclusion of enteropathogenic bacteria [26,27].”
BackgroundASSESSMENT OF POTENTIAL PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA AND YEAST STRAINS
Gotcheva, V., Hristozova, E., Hristozova, T., Guo, M., Рошкова, З., & Angelov, A. (2002) · Food Biotechnology · doi:10.1081/fbt-120016668 · Cited by 141 · see citing works
Nikolic, M., López, P., Strahinic, I., Suárez, A., Kojic, M., Fernández-García, M., Topisirovic, L., Golic, N., & Ruas-Madiedo, P. (2012) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2012.07.015 · Cited by 138 · cited 1× · see citing works
“Bacterial aggregation between cells of the same strain (autoaggregation) is of considerable importance and is one of the key factors to check the ability of the lactic acid bacterial strain to adhere to the oral cavity and gastrointestinal tract, where lactic acid bacteria are to be active and provide their benefi cia…”
BackgroundCharacterization of Lactobacillus isolated from dairy samples for probiotic properties
Kumar, A., & Kumar, D. (2015) · Anaerobe · doi:10.1016/j.anaerobe.2015.03.004 · Cited by 106 · cited 2× · see citing works
“Kumar and Kumar, (2015) [17], showed that LBS 2 colonies isolated from household curd were round, smooth, raised, entire margins, gram positive rods arranged in clusters, non-spore forming and non-motile.”
BackgroundMethodNovel Bacteriocin of Pediococcus sp. ISK‐1 Isolated from Well‐Aged Bed of Fermented Rice Bran
KIMURA, H., Sashihara, T., Matsusaki, H., Sonomoto, K., & ISHIZAKI, A. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb10336.x · Cited by 58 · see citing works
Zdolec, N., Hadžiosmanović, M., Kozačinski, L., Cvrtila, Ž., Filipović, I., Škrivanko, M., & Leskovar, K. (2008) · Meat Science · doi:10.1016/j.meatsci.2008.01.012 · Cited by 58 · cited 1× · see citing works
“(2008) [35], who also recorded Lactobacillus sp.”
Probiotic Research in Australia, New Zealand and the Asia-Pacific Region
Crittenden, R. G., Bird, A. R., Gopal, P. K., Henriksson, A., Lee, Y., & PLAYNE, M. J. (2005) · Current Pharmaceutical Design · doi:10.2174/1381612053382304 · Cited by 56 · cited 1× · see citing works
“(2005) [16] isolated lactic acid bacteria on MRS medium from different sources- commercial dairy food products viz.”
BackgroundHanda, S., & Sharma, N. (2016) · Journal of Genetic Engineering and Biotechnology · doi:10.1016/j.jgeb.2016.08.001 · Cited by 49 · cited 1× · see citing works
“The results obtained were well in agreement with that of Handa and Sharma, (2016) [34] in which Lactobacillus plantarum F22 was found to be sensitive to majority of tested antibiotics viz.”
ResultConjugative Gene Transfer in the Gastrointestinal Environment
Licht, T. R., & Wilcks, A. (2005) · Advances in applied microbiology · doi:10.1016/s0065-2164(05)58002-x · Cited by 45 · cited 1× · see citing works
“Indeed, the probiotic ability to adhere to the intestinal epithelium is regarded as a prerequisite to colonize the human GIT (gastrointestinal tract) for exerting benefi cial effects of probiotics such as the exclusion of enteropathogenic bacteria [26,27].”
BackgroundHaghshenas, B., Haghshenas, M., Nami, Y., Khosroushahi, A. Y., Abdullah, N., Barzegari, A., Rosli, R., & Hejazi, M. S. (2016) · PubMed Central · doi:10.15171/apb.2016.07 · Cited by 27 · cited 1× · see citing works
“plantarum 15HN and Enterococcus mundtii 50H displayed signifi cant anti-pathogenic activities against indicator microorganisms [21].”
BackgroundDonatien, K., tou, S. L. H. E., Mamoudou, H. D., hima, D. B., & Jakobsen, M. (2012) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb11.2667 · Cited by 20 · cited 1× · see citing works
“(2012) [31] measured sedimentation rate of L.”
Assessment of Probiotic Properties in Lactic Acid Bacteria Isolated from Fermented Vegetables
Savedboworn, W., Riansa-ngawong, W., Sinlapacharoen, W., Pajakang, S., Patcharajarukit, B., & Tipkanon, S. (2014) · King Mongkut’s University of Technology North Bangkok International Journal of Applied Science and Technology · doi:10.14416/j.ijast.2014.10.001 · Cited by 15 · cited 1× · see citing works
“(2014) [18] observed total 82 isolates of lactic acid bacteria from fermented vegetables on de Man, Rogosa and Sharpe (MRS) agar.”
Background
Flagged first, then by citations
Research works printed but not linked: 4
- Vasiee AR, Tabatabaei Yazdi F, Mortazavi A, Edalatian MR (2014) Isolation, identifi cation and characterization of probiotic Lactobacilli sp. from Tarkhineh. Int Food Res J 21(6): 2487-2492. Link: https://goo.gl/XekIKK
- CLSI (2007) Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing. 17th edition. Wayne, PA.
- Kumar A, Kumar D (2014) Isolation and characterization of bacteria from dairy samples of Solan in Himachal Pradesh for identifi cation of Lactobacillus spp. Int J Pharm Sci Rev Res 25(2): 110-114. Link: https://goo.gl/7vitcq
- Gupta A, Sharma N (2015) In vitro characterization of probiotic properties and detection of novel compounds secreted by lactic acid bacteria isolated from kodo millet (Paspalum scrobiculatum) fl our: A traditional Himalayan food. GERF Bulletin of Biosciences 6: 7-20. Link: https://goo.gl/6ho4Db
Cited by
Sorted by citations
Safety Evaluation and Colonisation Abilities of Four Lactic Acid Bacteria as Future Probiotics
Dlamini, Z. C., Langa, R. L. S., Aiyegoro, O. A., & Okoh, A. I. (2018) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-018-9430-y · Cited by 103 · see citing works
Golnari, M., Bahrami, N., Milanian, Z., Rabbani Khorasgani, M., Asadollahi, M. A., Shafiei, R., & Fatemi, S. S.-A. (2024) · Scientific Reports · doi:10.1038/s41598-024-51823-z · Cited by 86 · see citing works
“Sharma et al. suggested that an appropriate probiotic strain should withstand at least pH 3.0 34 , while Hong et al. reported that Bacillus strains exhibit different resistances to gastric acid and not all are resistant to these conditions 35 .”
InfluentialGoa, T., Beyene, G., Mekonnen, M., & Gorems, K. (2022) · International Journal of Food Science · doi:10.1155/2022/2076021 · Cited by 57 · see citing works
“The growth of isolates after 24 hours of incubation was determined by visual observing the turbidity [25].”
MethodSharma, K., Sharma, N., Handa, S., & Pathania, S. (2020) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-020-00063-5 · Cited by 51 · see citing works
Probiotic properties of lactic acid bacteria strains isolated from pastırma
Topçu, K. Ç., Kaya, M., & Kaban, G. (2020) · LWT · doi:10.1016/j.lwt.2020.110216 · Cited by 50 · see citing works
Karbowiak, M., Gałek, M., Szydłowska, A., & Zielińska, D. (2022) · Pathogens · doi:10.3390/pathogens11111260 · Cited by 35 · see citing works
“The cell’s ability to adhere to the intestinal epithelium is considered a prerequisite for colonization of the human gastrointestinal tract so probiotics or postbiotics can exhibit a range of beneficial effects such as the exclusion of enteropathogenic bacteria [38,39].”
BackgroundBentahar, M. C., Benabdelmoumene, D., Robert, V., Dahmouni, S., Qadi, W. S. M., Bengharbi, Z., Langella, P., Benbouziane, B., Al-Olayan, E. M., Dawoud, E. A. D., et al. (2024) · Foods · doi:10.3390/foods13233781 · Cited by 41 · see citing works
“Antibiotic sensitivity was evaluated using the Kirby–Bauer disc diffusion method on MRS agar [37].”
Yanez-Lemus, F., Moraga, R., Smith, C. T., Aguayo, P., Sánchez-Alonzo, K., García, A., Valenzuela, A. E., & Campos, V. L. (2022) · Biology · doi:10.3390/biology11101523 · Cited by 25 · see citing works
Simões, L., Fernandes, N., de Souza, A., dos Santos, L., Magnani, M., Abrunhosa, L., Teixeira, J., Schwan, R. F., & Dias, D. R. (2022) · Fermentation · doi:10.3390/fermentation8060277 · Cited by 23 · see citing works
Kumar, R., Bansal, P., Singh, J., Dhanda, S., & Bhardwaj, J. K. (2019) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2785-8 · Cited by 24 · see citing works
Modasiya, I. J., Mori, P., Maniya, H., Chauhan, M., Grover, C. R., Kumar, V., & Sarkar, A. K. (2024) · Food Science & Nutrition · doi:10.1002/fsn3.4537 · Cited by 20 · see citing works
Dessalegn, M., & Andualem, B. (2023) · International Journal of Food Properties · doi:10.1080/10942912.2023.2270177 · Cited by 11 · see citing works
“[15] Cell surface hydrophobicity The cell surface hydrophobicity of the LAB isolates were determined by following the procedure used by Sharma et al. [16] and Prabhurajeshwar and Chandrakanth.”
MethodHalder, D., & Mandal, S. (2018) · Bioscience Biotechnology Research Communications · doi:10.21786/bbrc/11.3/15 · Cited by 10 · see citing works
Rashid, H., Zaidi, A., Anwar, M. A., & Tariq, M. (2023) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2023.100812 · Cited by 7 · see citing works
Alp, D., & Kuleaşan, H. (2019) · GIDA / THE JOURNAL OF FOOD · doi:10.15237/gida.gd18059 · Cited by 5 · see citing works
Immobilized Plant-Based Presumptive Probiotics as Functional Ingredients for Breakfast Cereals
Pavlatou, C., Prapa, I., Stylianopoulou, E., Mitropoulou, G., Skavdis, G., & Kourkoutas, Y. (2025) · Fermentation · doi:10.3390/fermentation11060335 · Cited by 4 · see citing works
Chauhan, N., Kumar, S., Chauhan, T., & Samanta, A. K. (2024) · International Microbiology · doi:10.1007/s10123-024-00623-6 · Cited by 4 · see citing works
“These findings coincide with those of Collado et al. (Collado et al. 2008) and Sharma et al. (Sharma et al. 2018).”
InfluentialBenine, C., Boutlelis, D. A., Laiche, A. T., Amara, D. G., Khemakhem, M. M., Naccache, C., Atanassova, M., Zahnit, W., Ahmad, S. F., & Messaoudi, M. (2024) · International Journal of Food Properties · doi:10.1080/10942912.2024.2391399 · Cited by 3 · see citing works
“[40] Hong et al. [41] observed that different Bacillus strains display varying resistances to stomach acid and that not all strains are resistant to these conditions, while Sharma et al. [42] indicated that a good probiotic strain should survive at least pH 3.0.”
InfluentialTâm, H. N. T., Sương, P. T. T., & Diệp, N. L. H. (2019) · Can Tho University Journal of Science · doi:10.22144/ctu.jvn.2019.017 · Cited by 3 · see citing works
Rahmani, R., Ait-meddour, A., Benhoula, M., Hamma-Faradji, S., Belbachir, K., Terki, S., Calvigioni, M., Rossi, V., Mazzantini, D., Ghelardi, E., et al. (2026) · Current Microbiology · doi:10.1007/s00284-026-04777-6 · Cited by 1 · see citing works
Kaul, R., rimplekaul01@gmail.com., E., & Sharma, N. (2022) · MedPress Nutrition & Food Sciences · doi:10.33582/mpnfs.2022.202204003
wube, B. W., Habtu, T. A., Abera, A. A., Tenagashaw, M. W., Wudineh, T. A., Asres, D. T., Tiruneh, A. T., Awol, S. J., Bonger, Z. T., & Belete, M. A. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4795387 · Cited by 1 · see citing works
Nguyen, U. Q., Nguyễn, H. Q., Van, G., Phương, N. T., Dao, H., Nguyen, H. L., Le, H., & Hoang, V. (2022) · Vietnam Journal of Science and Technology/Science and Technology · doi:10.15625/2525-2518/16284
Lengur, E. R. A., Jatmiko, Y. D., Arisoesilaningsih, E., & Widodo, E. (2024) · AIP conference proceedings · doi:10.1063/5.0184040
Probiotics characterization of fermented tiger nut-millet-blended beverage
Odeleye, O. P., Gabriel-Ogunniyi, B. M., Adegbola, G. M., Gabriel-Ajobiewe, R. A., & Adeleke, B. S. (2026) · Journal of Food Bioactives · doi:10.26599/jfb.2026.95035456
- Authors
- Sharma, N.
- Affiliations
- Dr. Yashwant Singh Parmar University of Horticulture and Forestry (IN)
- Corresponding author
- Sharma, N
- ORCID
- Sharma, N
- Venue
- Journal of Clinical Microbiology and Biochemical Technology
- Published
- 2016-12-10
- Pages
- 018-025
- ISSN
- 2581-527X (ISSN-L)
- OpenAlex
- W2789869611
- Cited by
- 25
- References
- 25
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 7 · 2023: 2 · 2022: 6 · 2020: 2 · 2019: 3 · 2018: 2
- FWCI
- 2.10
- Type
- journal article
- Language
- en
- Update notices
- new_edition (2016-12-10)
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Clinical Microbiology and Biochemical Technology (publishedVersion, cc-by)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- lactic acid bacteria, probiotics, fermented dairy products, traditional dairy products, strain identification, Himachal Pradesh
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
