Cited by 108Probiotics and Fermented FoodsReproductive tract infections researchInfant Nutrition and Health

Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses

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Barzegar, Hassan, Alizadeh Behbahani, Behrooz, Falah, Fereshteh·2021·Food Science & Nutrition

Abstract

The objective of this study was to investigate probiotic, antimicrobial, technological and safety properties of lactobacillus strains isolated from local Iranian cheese made from raw milk. Six different samples were prepared, after serial dilution, culture was performed on MRS culture medium. The gram‐positive and catalase‐negative lactobacillus strains were subjected to grouping and identifying using biochemical tests, carbohydrates fermentation profiles, and 16S rDNA analysis. The results of sequence analysis showed the Lactobacillus spp. belonged to Lactobacillus brevis , Lactobacillus acidophilus , Lactobacillus plantarum, and Lactobacillus casei . After 3 hr incubation at pH=2, 3–6 log units of strains decreased which Lactobacillus acidophilus (B14) and Lactobacillus brevis (B2) showed highest resistance to low pH as well as simulated GIT juices. The highest and lowest hydrophobicity degree was belonged to L. acidophilus (B14) (65.9%) and L. casei (B22) (25.6%), respectively. Also, the highest auto‐aggregation and coaggregation were observed in L. acidophilus (B14) (51.3%) and L . plantarum (B20) (43.6%). The adhered percentage of strains varied from 2.5% to 14.6%. L. plantarum (B20) showed highest proteolytic activity followed by L. acidophilus (B14). Also, the highest autolytic activity belonged to L. acidophilus (B14). All of the strains showed low acidifying potential, except for L . acidophilus (B17) which decreased 2.05 unit of pH after 24 hr. The isolates did not show lipolytic activity as well as biogenic amines production (except L. brevis B3). All of the strains were sensitive to chloramphenicol and erythromycin except L. acidophilus (B15) and L. casei (B22). All strains showed no hemolysis activity which make them safe for consumption. Based on the obtained results, L . acidophilus (B14) presented the best probiotic and technological characteristics and is proposed for using as coculture in the dairy industrial.

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  • LABs are generally devoid of DNase activity, and their safety has been confirmed in this regard.

    “The results of extensive studies show that LABs are generally devoid of this activity, and their safety has been confirmed in this regard23,27.”

    From Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes (2026) · cites this paper as [27]

    The Safety evaluation and Safety attributes sections assess antibiotic susceptibility, hemolysis, and biogenic amine production, but do not address DNase activity. Searches for DNase activity, deoxyribonuclease, and DNA hydrolysis found no passage establishing that LAB generally lack DNase activity or are safe in that regard (page numbers unavailable). (LLM, conf=0.76)

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  1. Boldura, O.‐M., & Popescu, S. (2016). PCR: A Powerful Method in Food Safety Field. Biochemistry, Genetics and Molecular Biology" Polymerase Chain Reaction for Biomedical Applications" (pp. 135– 158). Intech Publishers.

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Authors
Barzegar, H., Alizadeh Behbahani, B., & Falah, F.
Affiliations
  • Agricultural Sciences and Natural Resources University of Khuzestan (IR)
  • Department of Food Science and Technology Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan Mollasani Iran
  • Ferdowsi University of Mashhad (IR)
Corresponding author
Barzegar, Hassan
Venue
Food Science & Nutrition
Publisher
Wiley
Published
2021-06-14
Volume
9
Issue
8
Pages
4094-4107
ISSN
2048-7177 (ISSN-L)
OpenAlex
W3169791977
Cited by
108
References
66
Citations by year
2026: 22 · 2025: 27 · 2024: 26 · 2023: 26 · 2022: 6 · 2021: 1
FWCI
8.81
Type
journal article
Language
en
Open access
Locations
  • Food Science & Nutrition (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
Lactobacillus, probiotics, raw milk cheese, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus casei, acid resistance, cell surface hydrophobicity, self-aggregation, cell adhesion, proteolytic activity, autolysis, antimicrobial susceptibility, hemolysis, biogenic amines, lipase activity, cheese starter cultures

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