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Nanodelivery of nucleic acids
Open accessAbstract
There is growing need for a safe, efficient, specific and non-pathogenic means for delivery of gene therapy materials. Nanomaterials for nucleic acid delivery offer an unprecedented opportunity to overcome these drawbacks; owing to their tunability with diverse physico-chemical properties, they can readily be functionalized with any type of biomolecules/moieties for selective targeting. Nucleic acid therapeutics such as antisense DNA, mRNA, small interfering RNA (siRNA) or microRNA (miRNA) have been widely explored to modulate DNA or RNA expression Strikingly, gene therapies combined with nanoscale delivery systems have broadened the therapeutic and biomedical applications of these molecules, such as bioanalysis, gene silencing, protein replacement and vaccines. Here, we overview how to design smart nucleic acid delivery methods, which provide functionality and efficacy in the layout of molecular diagnostics and therapeutic systems. It is crucial to outline some of the general design considerations of nucleic acid delivery nanoparticles, their extraordinary properties and the structure-function relationships of these nanomaterials with biological systems and diseased cells and tissues.
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“Modulation of surface characteris tics may help to improve transduction and reduce the dose required, thereby reducing the risk of GTAU (Mendes et al., 2022; Carvalho et al., 2023).”
From Gene Therapy-Associated Uveitis (GTAU): Understanding and mitigating the adverse immune response in retinal gene therapy (2025) · cites this paper as Mendes et al., 2022
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Cites
Vos, T., Lim, S. S., Abbafati, C., Abbas, K. M., Abbasi, M., Abbasifard, M., Abbasi-Kangevari, M., Abbastabar, H., Abd-Allah, F., Abdelalim, A., et al. (2020) · The Lancet · doi:10.1016/s0140-6736(20)30925-9 · Cited by 19,358
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine
Polack, F. P., Thomas, S. J., Kitchin, N., Absalon, J., Gurtman, A., Lockhart, S., Perez, J. L., Pérez Marc, G., Moreira, E. D., Zerbini, C., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2034577 · Cited by 14,592
Global Burden of Cardiovascular Diseases and Risk Factors, 1990–2019
Roth, G. A., Mensah, G. A., Johnson, C. O., Addolorato, G., Ammirati, E., Baddour, L. M., Barengo, N. C., Beaton, A. Z., Benjamin, E. J., Benziger, C. P., et al. (2020) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2020.11.010 · Cited by 11,449
Engineering precision nanoparticles for drug delivery
Mitchell, M. J., Billingsley, M. M., Haley, R. M., Wechsler, M. E., Peppas, N. A., & Langer, R. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-020-0090-8 · Cited by 7,933
Forouzanfar, M. H., Afshin, A., Alexander, L. T., Anderson, H. R., Bhutta, Z. A., Biryukov, S., Brauer, M., Burnett, R., Cercy, K., Charlson, F. J., et al. (2016) · The Lancet · doi:10.1016/s0140-6736(16)31679-8 · Cited by 7,698
Stimuli-responsive nanocarriers for drug delivery
Mura, S., Nicolas, J., & Couvreur, P. (2013) · Nature Materials · doi:10.1038/nmat3776 · Cited by 6,289
mRNA vaccines — a new era in vaccinology
Pardi, N., Hogan, M. J., Porter, F. W., & Weissman, D. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.243 · Cited by 4,667
Renal clearance of quantum dots
Soo Choi, H., Liu, W., Misra, P., Tanaka, E., Zimmer, J. P., Itty Ipe, B., Bawendi, M. G., & Frangioni, J. V. (2007) · Nature Biotechnology · doi:10.1038/nbt1340 · Cited by 4,476
Research works printed but not linked: 1
- Malaviya, M. & Shiroya, M. Systemic gene delivery using lipid envelope systems and its potential in overcoming challenges. Int. J. Pharm. Drug Anal. 9, 46–55 (2021).
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Ukwubile, C. A., Ahuchaogu, A. A., Robert, A. E., Yesufu, H. B., Odugu, J. A., Ida, P. A., & Ikpefan, E. O. (2026) · Clinical Phytoscience · doi:10.1186/s40816-026-00440-6
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Arikibe, J. E., Mazzetta, A. M., Casagrande, L., de Cotret, A. R., Tomlinson, C., Gris, D., & Caliceti, P. (2026) · Open Research Europe · doi:10.12688/openreseurope.24908.1
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Maharjan, R., Kim, G. N., Jung, H. S., Kim, H. J., Kim, J.-H., Kang, H. M., Kim, H. U., & Jeong, S. H. (2026) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2026.127476
Ou, Y., Zhang, J., Chu, Y., He, B., Lu, M., Liu, N., Zhang, H., Li, M., Liu, Y., Wang, Z., et al. (2026) · Nano Today · doi:10.1016/j.nantod.2026.103200
You, X., Xia, T., Guo, X., Wang, Q., Ren, X., Zeng, Q., Yao, C., & Yang, D. (2026) · Advanced Materials · doi:10.1002/adma.75257
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Fu, L., Ding, Z., Wu, J., Zhang, Z., 张智星, Long, C., Wu, J., Sui, X., Cao, Y., Liu, S., et al. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.71812
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Wang, S., Feng, J., Jia, J., Yan, T., Xiong, B., Liu, Y., Han, H., Hu, M., & Li, Q. (2026) · Journal of Controlled Release · doi:10.1016/j.jconrel.2026.115475
Xiao, H. P., 黎小霞, Cai, K., Leng, Y., Miao, X., Yin, T., Shuai, X., & Ren, J. (2026) · Science Advances · doi:10.1126/sciadv.aeh6590
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Li, J., Wang, Y., Liu, Y., 姬颖华, Lü, P., Yang, W., & Zhang, S. (2026) · Pharmacological Research · doi:10.1016/j.phrs.2026.109709
Sarpoli, L. M. G., Hamblin, M. R., & Ebrahimifard, M. (2026) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2026.2758938
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Liu, Y., Liu, Z., Chen, Q., Xia, X., Miao, K., Zhao, Q., Yang, H., Cui, S., Wang, W., Qin, C., et al. (2026) · Nano Biomedicine and Engineering · doi:10.1016/j.nbe.2026.100089
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Self-citation
Metadata
- Authors
- Mendes, B. B., Conniot, J., Avital, A., Yao, D., Jiang, X., Zhou, X., Sharf-Pauker, N., Xiao, Y., Adir, O., Liang, H., Shi, J., Schroeder, A., & Conde, J.
- Affiliations
- Ningbo University (CN)
- University of Science and Technology of China (CN)
- Brigham and Women's Hospital (US)
- Technion – Israel Institute of Technology (IL)
- Universidade Nova de Lisboa (PT)
- Corresponding author
- Liang, Haojun
- Shi, Jinjun
- Schroeder, Avi
- Conde, João
- ORCID
- Venue
- Nature Reviews Methods Primers
- Published
- 2022-04-14
- Volume
- 2
- Issue
- 1
- ISSN
- 2662-8449 (ISSN-L)
- PMC
- PMC9038125
- OpenAlex
- W4223968391
- Cited by
- 719
- References
- 228
- Citations by year
- 2026: 166 · 2025: 233 · 2024: 196 · 2023: 103 · 2022: 21
- FWCI
- 14.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nature Reviews Methods Primers (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Funders
- U.S. Department of Veterans Affairs (I01 BX004426)
- American Lung Association
- Massachusetts General Hospital
- National Natural Science Foundation of China (52003264, 21991132, 52033010, 2020YFA0710700, 52021002)
- National Institutes of Health
- Fundação para a Ciência e a Tecnologia (PTDC/BTM-MAT/4738/2020)
- Fundamental Research Funds for the Central Universities
- National Heart, Lung, and Blood Institute (R01HL159012, R01HL156362, R01HL137229, R01HL158097, R01HL162367)
- National Cancer Institute (R01CA200900)
- H2020 European Research Council (848325)
- DOD Peer Reviewed Cancer Research Program
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- nucleic acid delivery, nanodelivery, gene therapy, nanoparticles, siRNA, microRNA, antisense DNA, mRNA therapeutics, targeted delivery, structure-function relationship, molecular diagnosis, gene silencing, vaccines, protein replacement
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