A Hitchhiker’s guide to CRISPR editing tools in bacteria: CRISPR can help unlock the bacterial world, but technical and regulatory barriers persist
Open accessAbstract
Join us on a journey through the complex and ever-expanding universe of CRISPR approaches for genome editing in bacteria. Discover what is available, current technical challenges, successful implementation of these tools and the regulatory framework around their use. [Image: see text]
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The development of CRISPR technologies has significantly improved the efficiency and accuracy of genome engineering relative to traditional homologous recombination techniques.
“The development of CRISPR technologies has significantly improved the efficiency and accuracy of genome engineering relative to traditional homologous recombination techniques [113,114].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [113]
In “Start with the basics” (page unavailable), the paper explains that CRISPR cutting can enrich for cells edited by recombineering and describes newer precision-editing tools. It does not establish a significant improvement in both efficiency and accuracy over traditional homologous recombination; it describes CRISPR and recombineering as commonly used together, and notes that editing efficiency can vary widely. (LLM, conf=0.85)
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Cites
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- Authors
- Krink, N., Nikel, P. I., & Beisel, C. L.
- Affiliations
- Novo Nordisk Foundation (DK)
- University of Würzburg (DE)
- Corresponding author
- Beisel, Chase L
- Venue
- EMBO Reports
- Published
- 2024-02-12
- Volume
- 25
- Issue
- 4
- Pages
- 1694-1699
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- PMC
- PMC11014848
- OpenAlex
- W4391752909
- Cited by
- 19
- References
- 12
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 4
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- EMBO Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Novo Nordisk Fonden (NNF20OC0065068, NNF20CC0035580, NNF18OC0034818, NNF21OC0067996)
- EC | Horizon 2020 Framework Programme (814418)
- EC | ERC | HORIZON EUROPE European Research Council (865973)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- CRISPR, genome editing, bacteria, CRISPR-Cas, genome editing tools, technical challenges, regulatory framework, gene editing regulation
- MeSH terms
- Gene Editing, Bacteria, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
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