An RNA-Seq atlas of gene expression in mouse and rat normal tissues
Open accessAbstract
Gene functionality is closely connected to its expression specificity across tissues and cell types. RNA-Seq is a powerful quantitative tool to explore genome wide expression. The aim of this study is to provide a comprehensive RNA-Seq dataset across the same 13 tissues for mouse and rat, two of the most relevant species for biomedical research. The dataset provides the transcriptome across tissues from three male C57BL6 mice and three male Han Wistar rats. We also describe our bioinformatics pipeline to process and technically validate the data. Principal component analysis shows that tissue samples from both species cluster similarly. We show by comparative genomics that many genes with high sequence identity with respect to their human orthologues also have a highly correlated tissue distribution profile and are in agreement with manually curated literature data for human. In summary, the present study provides a unique resource for comparative genomics and will facilitate the analysis of tissue specificity and cross-species conservation in higher organisms.
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- Authors
- Söllner, J. F., Leparc, G., Hildebrandt, T., Klein, H., Thomas, L., Stupka, E., & Simon, E.
- Affiliations
- Boehringer Ingelheim (Germany) (DE)
- Corresponding author
- Simon, Eric
- Venue
- Scientific Data
- Published
- 2017-12-12
- Volume
- 4
- Issue
- 1
- Pages
- 170185-170185
- ISSN
- 2052-4463 (ISSN-L)
- PMC
- PMC5726313
- OpenAlex
- W2773852483
- Cited by
- 133
- References
- 23
- Citations by year
- 2026: 4 · 2025: 19 · 2024: 19 · 2023: 14 · 2022: 24 · 2021: 15 · 2020: 16 · 2019: 18 · 2018: 4
- Type
- data paper
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Data (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Keywords
- tissue-specific gene expression, transcriptome, mice, rats, comparative genomics, human orthologs, cross-species conservation, C57BL/6 mice, Wistar rats
- MeSH terms
- Animals, Organ Specificity, RNA, Sequence Analysis, RNA, Genomics, Mice, Rats, Transcriptome
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