The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Apocynin inhibits peroxynitrite formation by murine macrophages
Open accessAbstract
Peroxynitrite (ONOO−) the highly reactive coupling product of nitric oxide and superoxide, has been implicated in the pathogenesis of an increasing number of (inflammatory) diseases. At present, however, selective peroxynitrite antagonizing agents with therapeutic potential are not available. Therefore, the NADPH‐oxidase inhibitor apocynin (4‐hydroxy‐3‐methoxy‐acetophenone) was tested for its ability to inhibit peroxynitrite formation in vitro The murine macrophage cell‐line J774A.1, stimulated with IFNγ/LPS, was used as a model. Conversion of 123‐dihydrorhodamine (123‐DHR) to its oxidation product 123‐rhodamine was used to measure peroxynitrite production. Stimulated peroxynitrite formation could be completely inhibited by apocynin, by the superoxide scavenger TEMPO as well as by the nitric oxide synthase inhibitor aminoguanidine. Apocynin and aminoguanidine specifically inhibited superoxide and nitric oxide formation respectively as confirmed by measuring lucigenin enhanced chemiluminescence and nitrite accumulation. It is concluded that J774A.1 macrophages produce significant amounts of peroxynitrite, which is associated with nitric oxide production and NADPH‐oxidase dependent superoxide formation. The NADPH‐oxidase inhibitor apocynin proved to be a potent inhibitor of both superoxide and peroxynitrite formation by macrophages, which may be of future therapeutic significance in a wide range of inflammatory disorders. British Journal of Pharmacology (2000) 130, 932–936; doi:10.1038/sj.bjp.0703401
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Cites
Formation of nitric oxide-derived inflammatory oxidants by myeloperoxidase in neutrophils
Eiserich, J. P., Hristova, M., Cross, C. E., Jones, A. D., Freeman, B. A., Halliwell, B., & van der Vliet, A. (1998) · Nature · doi:10.1038/34923 · Cited by 1,496
Direct EPR Detection of the Carbonate Radical Anion Produced from Peroxynitrite and Carbon Dioxide
Bonini, M. G., Radi, R., Ferrer-Sueta, G., Ferreira, A. M. D. C., & Augusto, O. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.16.10802 · Cited by 293
The formation of peroxynitrite in vivo from nitric oxide and superoxide
Squadrito, G. L., & Pryor, W. A. (1995) · Chemico-Biological Interactions · doi:10.1016/0009-2797(94)03591-u · Cited by 200
Cited by
Chen, T., Hu, Y., Cen, Y., Chu, X., & Lu, Y. (2013) · Journal of the American Chemical Society · doi:10.1021/ja4035939 · Cited by 308
Sakellariou, G. K., Vasilaki, A., Palomero, J., Kayani, A., Zibrik, L., McArdle, A., & Jackson, M. J. (2013) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.4623 · Cited by 276
Subramanian Vignesh, K., Landero Figueroa, J. A., Porollo, A., Caruso, J. A., & Deepe, G. S. (2013) · Immunity · doi:10.1016/j.immuni.2013.09.006 · Cited by 230
Inhibition of human vascular nadph oxidase by apocynin derived oligophenols
Mora-Pale, M., Weïwer, M., Yu, J., Linhardt, R. J., & Dordick, J. S. (2009) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2009.05.061 · Cited by 36
Wan, J., Bae, M.-A., & Song, B.-J. (2004) · Experimental & Molecular Medicine · doi:10.1038/emm.2004.10 · Cited by 32
Metadata
- Authors
- Muijsers, R. B. R., Van Den Worm, E., Folkerts, G., Beukelman, C. J., Koster, A. S., Postma, D. S., & Nijkamp, F. P.
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 2000-06
- Volume
- 130
- Issue
- 4
- Pages
- 932-936
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- OpenAlex
- W2003554652
- Cited by
- 160
- References
- 3
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- green
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