The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Block of TRPC5 channels by 2‐aminoethoxydiphenyl borate: a differential, extracellular and voltage‐dependent effect
Open accessAbstract
2‐Aminoethoxydiphenyl borate (2‐APB) has been widely used to examine the roles of inositol 1,4,5‐trisphosphate receptors (IP 3 Rs) and store‐operated Ca 2+ entry and is an emerging modulator of cationic channels encoded by transient receptor potential (TRP) genes. Using Ca 2+‐indicator dye and patch‐clamp recording we first examined the blocking effect of 2‐APB on human TRPC5 channels expressed in HEK‐293 cells. The concentration–response curve has an IC 50 of 20 μM and slope close to 1.0, suggesting one 2‐APB molecule binds per channel. The blocking effect is not shared by other Ca 2+ channel blockers including methoxyverapamil, nifedipine, N‐propargylnitrendipine, or berberine. In whole‐cell and excised membrane patch recordings, 2‐APB acts from the extracellular but not intracellular face of the membrane. Block of TRPC5 by 2‐APB is less at positive voltages, suggesting that it enters the electric field or acts by modulating channel gating. 2‐APB also blocks TRPC6 and TRPM3 expressed in HEK‐293 cells, but not TRPM2. Block of TRP channels by 2‐APB may be relevant to cell proliferation because 2‐APB has a greater inhibitory effect on proliferation in cells overexpressing TRPC5. Our data indicate a specific and functionally important binding site on TRPC5 that enables block by 2‐APB. The site is only available via an extracellular route and the block shows mild voltage‐dependence. British Journal of Pharmacology (2005) 145, 405–414. doi:10.1038/sj.bjp.0706197
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
TRP channels as cellular sensors
Clapham, D. E. (2003) · Nature · doi:10.1038/nature02196 · Cited by 2,779
ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology
Perraud, A.-L., Fleig, A., Dunn, C. A., Bagley, L. A., Launay, P., Schmitz, C., Stokes, A. J., Zhu, Q., Bessman, M. J., Penner, R., et al. (2001) · Nature · doi:10.1038/35079100 · Cited by 892
TRPC1 and TRPC5 Form a Novel Cation Channel in Mammalian Brain
Strübing, C., Krapivinsky, G., Krapivinsky, L., & Clapham, D. E. (2001) · Neuron · doi:10.1016/s0896-6273(01)00240-9 · Cited by 738
Polycystin-2 is an intracellular calcium release channel
Koulen, P., Cai, Y., Geng, L., Maeda, Y., Nishimura, S., Witzgall, R., Ehrlich, B. E., & Somlo, S. (2002) · Nature Cell Biology · doi:10.1038/ncb754 · Cited by 706
Bootman, M. D., Collins, T. J., Mackenzie, L., Roderick, H. L., Berridge, M. J., & Peppiatt, C. M. (2002) · The FASEB Journal · doi:10.1096/fj.02-0037rev · Cited by 692
Requirement of the Inositol Trisphosphate Receptor for Activation of Store-Operated Ca 2+ Channels
Ma, H.-T., Patterson, R. L., van, D. B., Rossum, Birnbaumer, L., Mikoshiba, K., & Gill, D. L. (2000) · Science · doi:10.1126/science.287.5458.1647 · Cited by 577
2-Aminoethoxydiphenyl Borate Is a Common Activator of TRPV1, TRPV2, and TRPV3
Hu, H.-Z., Gu, Q., Wang, C., Colton, C. K., Tang, J., Kinoshita-Kawada, M., Lee, L.-Y., Wood, J. D., & Zhu, M. X. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m404164200 · Cited by 517
Prakriya, M., & Lewis, R. S. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.t01-1-00003.x · Cited by 493
TRPC5 is a regulator of hippocampal neurite length and growth cone morphology
Greka, A., Navarro, B., Oancea, E., Duggan, A., & Clapham, D. E. (2003) · Nature Neuroscience · doi:10.1038/nn1092 · Cited by 382
Molecular and Functional Characterization of the Melastatin-related Cation Channel TRPM3
Grimm, C., Kraft, R., Sauerbruch, S., Schultz, G., & Harteneck, C. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300945200 · Cited by 367
Self-citation
Cited by
Metalloimmunology: The metal ion-controlled immunity
(2020) · Advances in Immunology · doi:10.1016/bs.ai.2019.11.007 · Cited by 292
Juszczak, G. R., & Swiergiel, A. H. (2009) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2008.12.014 · Cited by 211
Martin, A. C. L., Willoughby, D., Ciruela, A., Ayling, L.-J., Pagano, M., Wachten, S., Tengholm, A., & Cooper, D. M. F. (2009) · Molecular Pharmacology · doi:10.1124/mol.108.051748 · Cited by 75
Metadata
- Authors
- Xu, S., Zeng, F., Boulay, G., Grimm, C., Harteneck, C., & Beech, D. J.
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 2005-06
- Volume
- 145
- Issue
- 4
- Pages
- 405-414
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- OpenAlex
- W1840415699
- Cited by
- 258
- References
- 32
- FWCI
- 7.04
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Block of TRPC5 channels by 2‐aminoethoxydiphenyl borate: a differential, extracellular and voltage‐dependent effect. https://app.cochat.ai/papers/10.1038/sj.bjp.0706197
- The paper
- Xu, S., Zeng, F., Boulay, G., Grimm, C., Harteneck, C., & Beech, D. J. (2005). Block of TRPC5 channels by 2‐aminoethoxydiphenyl borate: a differential, extracellular and voltage‐dependent effect. British Journal of Pharmacology. https://doi.org/10.1038/sj.bjp.0706197
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
