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Aberrant expression and function of TCF4 in the proliferation of hepatocellular carcinoma cell line BEL-7402
Abstract
Wnt signaling pathway is essential for development and tumorigenesis, however, this signaling pathway in the progress of hepatocellular carcinoma (HCC) remains unclear. In this paper, we studied the function of human T-cell transcription factor-4 (TCF4), a key factor of Wnt signaling pathway, on the proliferation of HCC cell line. We showed that the expression of TCF4 mRNA in HCC cell line BEL-7402 was higher than that in immortalized normal liver cell line L02. Blockage of Wnt pathway by Delta-NTCF4, a dominant negative TCF4, could suppress BEL-7402 cells growth and decrease the expression of cyclin D1 and c-myc, two of target genes of Wnt pathway. On the other hand, stimulating Wnt pathway by introducing a degradation-resistant -catenin S37A could increase BEL-7402 cells proliferation. But all the treatments had no effect on L02 cells. Our data indicated that TCF4 might be another key factor in Wnt pathway involved in HCC cells proliferation and TCF4 could be an effective therapeutic target for suppressing the growth of hepatocellular cancers.
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TCF4 over-expression was previously related to classical Wnt-target-genes expression (e.g. CCNDs or MMPs).
“Regarding TCF4 over-expression, it was previously related to classical Wnt-target-genes expression (e.g. CCNDs or MMPs), even though isoform specific functions remain unclear and would require further analysis (30).”
From Wnt-Pathway Activation in Two Molecular Classes of Hepatocellular Carcinoma and Experimental Modulation by Sorafenib (2012) · cites this paper as [30]
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Metadata
- Authors
- ZHAO, D. H., HONG, J. J., GUO, S. Y., YANG, R. L., YUAN, J., WEN, C. J., ZHOU, K. Y., & LI, C. J.
- Affiliations
- Nanjing Normal University (CN)
- Venue
- Cell Research
- Published
- 2004-02
- Volume
- 14
- Issue
- 1
- Pages
- 74-80
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- OpenAlex
- W1971674609
- Cited by
- 39
- References
- 24
- Citations by year
- 2026: 2 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 1 · 2017: 2 · 2016: 5 · 2014: 1 · 2013: 1 · 2012: 4
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Government of Jiangsu Province
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- TCF4, Wnt signaling, hepatocellular carcinoma, BEL-7402 cells, cell proliferation, cyclin D1, c-Myc, therapeutic target
- MeSH terms
- Cell Division, Cell Line, Cytoplasm, Cytoskeletal Proteins, DNA-Binding Proteins, Genetic Vectors, Carcinoma, Hepatocellular, Humans, Immunohistochemistry, Liver Neoplasms, Microscopy, Fluorescence, Mutation, Proto-Oncogene Proteins, Spectrometry, Fluorescence, Transcription Factors, Transfection, Blotting, Western, Signal Transduction, Trans-Activators, Gene Expression, Gene Expression Regulation, Neoplastic, Proto-Oncogene Proteins c-myc, Genes, Reporter, Cyclin D1, Reverse Transcriptase Polymerase Chain Reaction, Cell Line, Tumor, Wnt Proteins, beta Catenin, TCF Transcription Factors, Lymphoid Enhancer-Binding Factor 1, Transcription Factor 7-Like 2 Protein
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