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Receptor and viral determinants of SARS‐coronavirus adaptation to human ACE2

Open access
Li, Wenhui, Zhang, Chengsheng, Sui, Jianhua, Kuhn, Jens H, Moore, Michael J, Luo, Shiwen, Wong, Swee‐Kee, Huang, I‐Chueh, Xu, Keming, Vasilieva, Natalya, Murakami, Akikazu, He, Yaqing, Marasco, Wayne A, Guan, Yi, Choe, Hyeryun, Farzan, Michael·2005·The EMBO Journal
DOI 10.1038/sj.emboj.7600640
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Abstract

Human angiotensin‐converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS‐CoV). Here we identify the SARS‐CoV spike (S)‐protein‐binding site on ACE2. We also compare S proteins of SARS‐CoV isolated during the 2002–2003 SARS outbreak and during the much less severe 2003–2004 outbreak, and from palm civets, a possible source of SARS‐CoV found in humans. All three S proteins bound to and utilized palm‐civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S‐protein‐binding site of human ACE2 to those of civet ACE2, or by altering S‐protein residues 479 and 487 to residues conserved during the 2002–2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS‐CoV to human cells, and provide insight into the severity of the 2002–2003 SARS epidemic.

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Metadata

Authors
Li, W., Zhang, C., Sui, J., Kuhn, J. H., Moore, M. J., Luo, S., Wong, S., Huang, I., Xu, K., Vasilieva, N., Murakami, A., He, Y., Marasco, W. A., Guan, Y., Choe, H., & Farzan, M.
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2005-03-24
Volume
24
Issue
8
Pages
1634-1643
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.1038/sj.emboj.7600640
OpenAlex
W2038918691
Cited by
988
References
4
FWCI
19.61
Type
journal article
Language
en
Open access
green
Licence

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