The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Replication‐independent chromatin loading of Dnmt1 during G2 and M phases
Open accessAbstract
The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond S phase. Using time‐lapse microscopy of mammalian cells expressing green‐fluorescent‐protein‐tagged Dnmt1 and DsRed‐tagged DNA Ligase I as a cell cycle progression marker, we have found that Dnmt1 associates with chromatin during G2 and M. This association is mediated by a specific targeting sequence, shows strong preference for constitutive but not facultative heterochromatin and is independent of heterochromatin‐specific histone H3 Lys 9 trimethylation, SUV39H and HP1. Moreover, photobleaching analyses showed that Dnmt1 is continuously loaded onto chromatin throughout G2 and M, indicating a replication‐independent role of Dnmt1 that could represent a novel and separate pathway to maintain DNA methylation.
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Okano, M., Bell, D. W., Haber, D. A., & Li, E. (1999) · Cell · doi:10.1016/s0092-8674(00)81656-6 · Cited by 6,143
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
Li, E., Bestor, T. H., & Jaenisch, R. (1992) · Cell · doi:10.1016/0092-8674(92)90611-f · Cited by 3,984
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
Lachner, M., O'Carroll, D., Rea, S., Mechtler, K., & Jenuwein, T. (2001) · Nature · doi:10.1038/35065132 · Cited by 2,868
Cited by
UHRF1 Plays a Role in Maintaining DNA Methylation in Mammalian Cells
Bostick, M., Kim, J. K., Estève, P.-O., Clark, A., Pradhan, S., & Jacobsen, S. E. (2007) · Science · doi:10.1126/science.1147939 · Cited by 1,455
A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins
Rothbauer, U., Zolghadr, K., Muyldermans, S., Schepers, A., Cardoso, M. C., & Leonhardt, H. (2008) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700342-mcp200 · Cited by 677
Self-citationEpigenetic mechanisms in neurological disease
Jakovcevski, M., & Akbarian, S. (2012) · Nature Medicine · doi:10.1038/nm.2828 · Cited by 450
Ghoshal, K., Datta, J., Majumder, S., Bai, S., Kutay, H., Motiwala, T., & Jacob, S. T. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.11.4727-4741.2005 · Cited by 431
RetractedMutations in DNMT1 cause hereditary sensory neuropathy with dementia and hearing loss
Klein, C. J., Botuyan, M.-V., Wu, Y., Ward, C. J., Nicholson, G. A., Hammans, S., Hojo, K., Yamanishi, H., Karpf, A. R., Wallace, D. C., et al. (2011) · Nature Genetics · doi:10.1038/ng.830 · Cited by 405
Metadata
- Authors
- Easwaran, H. P., Schermelleh, L., Leonhardt, H., & Cardoso, M. C.
- Venue
- EMBO reports
- Published
- 2004-12
- Volume
- 5
- Issue
- 12
- Pages
- 1181-1186
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2071762384
- Cited by
- 182
- References
- 3
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- green
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