The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins
Open accessAbstract
Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics. We engineered a specific binder for fluorescent proteins based on a 13-kDa GFP binding fragment derived from a llama single chain antibody. This GFP-binding protein (GBP) can easily be produced in bacteria and coupled to a monovalent matrix. The GBP allows a fast and efficient (one-step) isolation of GFP fusion proteins and their interacting factors for biochemical analyses including mass spectroscopy and enzyme activity measurements. Moreover GBP is also suitable for chromatin immunoprecipitations from cells expressing fluorescent DNA-binding proteins. Most importantly, GBP can be fused with cellular proteins to ectopically recruit GFP fusion proteins allowing targeted manipulation of cellular structures and processes in living cells. Because of the high affinity capture of GFP fusion proteins in vitro and in vivo and a size in the lower nanometer range we refer to the immobilized GFP-binding protein as GFP-nanotrap. This versatile GFP-nanotrap enables a unique combination of microscopic, biochemical, and functional analyses with one and the same protein.
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1 retracted
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Naturally occurring antibodies devoid of light chains
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Self-citationA monomeric red fluorescent protein
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Nanobodies: natural single-domain antibodies
Muyldermans, S. (2013) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-063011-092449 · Cited by 2,304
Self-citationInsights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins
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Modulation of protein properties in living cells using nanobodies
Kirchhofer, A., Helma, J., Schmidthals, K., Frauer, C., Cui, S., Karcher, A., Pellis, M., Muyldermans, S., Casas-Delucchi, C. S., Cardoso, M. C., et al. (2010) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1727 · Cited by 586
Self-citationFluorescent fusion protein knockout mediated by anti-GFP nanobody
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Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor
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Metadata
- Authors
- Rothbauer, U., Zolghadr, K., Muyldermans, S., Schepers, A., Cardoso, M. C., & Leonhardt, H.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2008-02
- Volume
- 7
- Issue
- 2
- Pages
- 282-289
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2028544866
- Cited by
- 677
- References
- 39
- FWCI
- 9.73
- Type
- journal article
- Language
- en
- Open access
- hybrid
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by/4.0/ (vor, 4585 d)
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- Rothbauer, U., Zolghadr, K., Muyldermans, S., Schepers, A., Cardoso, M. C., & Leonhardt, H. (2008). A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.m700342-mcp200
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