The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
In vivo gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium
Abstract
We have evaluated the transduction profiles of an HIV-based lentiviral vector delivered regionally to ocular tissues in vivo. Following subretinal injection, a green fluorescent protein (GFP) reporter gene was efficiently and stably expressed in retinal pigment epithelial (RPE) cells. Limited transduction of adjacent photoreceptors occurred in newborn mice, but was inefficient in adult animals. Injection of the vector into the anterior chamber resulted in efficient and stable transduction of corneal endothelial cells. Efficient in vivo gene transfer into cells of the corneal endothelium and retinal pigment epithelium by lentiviral vectors may therefore offer a valuable approach to the treatment of disorders of the cornea and outer retina.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In another study, HIV vectors carrying GFP were delivered by subretinal injection-a stable expression was observed in RPE, and no inflammatory cell infiltration was observed by immunostaining [57].”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [57]
Citations
Cites
Self-Inactivating Lentivirus Vector for Safe and Efficient In Vivo Gene Delivery
Zufferey, R., Dull, T., Mandel, R. J., Bukovsky, A., Quiroz, D., Naldini, L., & Trono, D. (1998) · Journal of Virology · doi:10.1128/jvi.72.12.9873-9880.1998 · Cited by 1,815
HIV-1 Genome Nuclear Import Is Mediated by a Central DNA Flap
Zennou, V., Petit, C., Guétard, D., Nerhbass, U., Montagnier, L., & Charneau, P. (2000) · Cell · doi:10.1016/s0092-8674(00)80828-4 · Cited by 864
Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector
Miyoshi, H., Takahashi, M., Gage, F. H., & Verma, I. M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.19.10319 · Cited by 497
Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus
Flannery, J. G., Zolotukhin, S., Vaquero, M. I., LaVail, M. M., Muzyczka, N., & Hauswirth, W. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6916 · Cited by 409
Gene transfer into the mouse retina mediated by an adeno-associated viral vector
Ali, R. R. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.5.591 · Cited by 258
Self-citationCell cycle protein expression and proliferative status in human corneal cells.
Joyce, N. C., Meklir, B., Joyce, S. J., & Zieske, J. D. (1996) · PubMed · Cited by 251
Immune Responses Limit Adenovirally Mediated Gene Expression in the Adult Mouse Eye
Reichel, M., Ali, R., Thrasher, A., Hunt, D., Bhattacharya, S., & Baker, D. (1998) · Gene Therapy · doi:10.1038/sj.gt.3300691 · Cited by 106
Self-citationEfficient and sustained transgene expression in human corneal cells mediated by a lentiviral vector
Wang, X.-Y., Appukuttan, B., Ott, S. H., Patel, R. J., Irvine, J. A., Song, J., Park, J.-H. C., Smith, R. R., & Stout, J. T. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301075 · Cited by 94
The cellular basis of aqueous outflow regulation
Francis, B. A., & Alvarado, J. A. (1997) · Current Opinion in Ophthalmology · doi:10.1097/00055735-199704000-00005 · Cited by 25
Galileo, D. S., Hunter, K., & Smith, S. B. (1999) · Current Eye Research · doi:10.1076/ceyr.18.2.135.5376 · Cited by 20
Research works printed but not linked: 1
- Green K . Corneal endothelial structure and function under normal and toxic conditions (published erratum appears in Cell Biol Rev 1991; 25: 343) Cell Biol Rev 1991 25: 169–207
Cited by
Sorted by citations
Gaudana, R., Ananthula, H. K., Parenky, A., & Mitra, A. K. (2010) · The AAPS Journal · doi:10.1208/s12248-010-9183-3 · Cited by 1,406
Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
Schmidt, F., Weisblum, Y., Muecksch, F., Hoffmann, H.-H., Michailidis, E., Lorenzi, J. C. C., Mendoza, P., Rutkowska, M., Bednarski, E., Gaebler, C., et al. (2020) · Journal of Experimental Medicine · doi:10.1084/jem.20201181 · Cited by 572
MX2 is an interferon-induced inhibitor of HIV-1 infection
Kane, M., Yadav, S. S., Bitzegeio, J., Kutluay, S. B., Zang, T. M., Wilson, S. J., Schoggins, J. W., Rice, C. M., Yamashita, M., Hatziioannou, T., et al. (2013) · Nature · doi:10.1038/nature12653 · Cited by 510
Effective gene therapy with nonintegrating lentiviral vectors
Yáñez-Muñoz, R. J., Balaggan, K. S., MacNeil, A., Howe, S. J., Schmidt, M., Smith, A. J., Buch, P., MacLaren, R. E., Anderson, P. N., Barker, S. E., et al. (2006) · Nature Medicine · doi:10.1038/nm1365 · Cited by 460
Self-citationTrim5α protein restricts both HIV-1 and murine leukemia virus
Yap, M. W., Nisole, S., Lynch, C., & Stoye, J. P. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402876101 · Cited by 455
Metadata
- Authors
- Bainbridge, J., Stephens, C., Parsley, K., Demaison, C., Halfyard, A., Thrasher, A., & Ali, R.
- Affiliations
- University College London (GB)
- Venue
- Gene Therapy
- Published
- 2001-11-01
- Volume
- 8
- Issue
- 21
- Pages
- 1665-1668
- ISSN
- 0969-7128 (ISSN-L)
- 1476-5462
- OpenAlex
- W1972583115
- Cited by
- 197
- References
- 13
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 9 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 8 · 2016: 3 · 2015: 10 · 2014: 6 · 2013: 14 · 2012: 7
- FWCI
- 4.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene Therapy (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- lentiviral vectors, HIV-based vectors, subretinal injection, anterior chamber injection, retinal pigment epithelium, corneal endothelium, photoreceptors, GFP expression, corneal disease, mouse eye
- MeSH terms
- Animals, Animals, Newborn, Eye, Eye Diseases, Genetic Vectors, Luminescent Proteins, Mice, Inbred C57BL, Microinjections, Pigment Epithelium of Eye, Transduction, Genetic, Genetic Therapy, HIV-1, Gene Expression, Epithelium, Corneal, Green Fluorescent Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
