Single-walled carbon nanotubes-mediated in vivo and in vitro delivery of siRNA into antigen-presenting cells
Abstract
Antigen-presenting cells such as dendritic cells (DCs) play a critical role in inducing and regulating immune responses. One effective strategy for DC-based immunotherapy is to regulate maturation and function of DC. In this study, we apply single-walled carbon nanotubes (SWNTs) to carry small interfering RNA (siRNA) to reach, enter and genetically modify DCs in vivo. We prepared positively charged SWNTs (SWNTs+) using 1,6-diaminohexane which was demonstrated by transmission electron microscopy equipped with energy-dispersive X-ray spectroscopy and atomic force microscope. The functionalized SWNTs+ could absorb siRNA to form complexes of siRNA with SWNTs. These siRNA:SWNT+ complexes were preferentially taken up by splenic CD11c+ DCs, CD11b+ cells and also Gr-1+CD11b+ cells comprising DCs, macrophages and other myeloid cells to silence the targeting gene. Suppressor of cytokine signaling 1 (SOCS1) restricts the ability of DCs to break self-tolerance and induce antitumor immunity. Infusion of SWNTs+ carrying SOCS1siRNA reduced SOCS1 expression and retarded the growth of established B16 tumor in mice, indicating the possibility of in vivo immunotherapeutics using SWNTs-based siRNA transfer system.
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CNTs have shown promise in carrying plasmid DNA, small interfering RNA, antisense oligonucleotides, and aptamers.
“CNTs have shown promise in carrying plasmid DNA, small interfering ribonucleic acid, antisense oligonucleotides, and aptamers.114”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [114]
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- Authors
- Yang, R., Yang, X., Zhang, Z., Zhang, Y., Wang, S., Cai, Z., Jia, Y., Ma, Y., Zheng, C., Lu, Y., Roden, R., & Chen, Y.
- Affiliations
- Johns Hopkins University (US)
- Nankai University (CN)
- Tianjin Chest Hospital (CN)
- Civil Aviation University of China (CN)
- Corresponding author
- Yang, R
- Chen, Y
- Venue
- Gene Therapy
- Published
- 2006-07-06
- Volume
- 13
- Issue
- 24
- Pages
- 1714-1723
- ISSN
- 0969-7128 (ISSN-L)
- 1476-5462
- OpenAlex
- W2039641449
- Semantic Scholar
- b6e6534302fb210c2902dac8f427abf3a2b637c7
- Cited by
- 98
- References
- 32
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 6 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 14 · 2012: 9
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- Open access
- closed
- Locations
- Gene Therapy (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Physical Sciences › Engineering › Biomedical Engineering › Graphene and Nanomaterials Applications
- Keywords
- single-walled carbon nanotubes, siRNA delivery, antigen-presenting cells, dendritic cells, SOCS1, gene silencing, myeloid cells, macrophages, cancer immunotherapy, B16 melanoma, DC-based immunotherapy, nanoparticle-mediated gene delivery
- MeSH terms
- Suppressor of Cytokine Signaling 1 Protein, Animals, Antigen-Presenting Cells, Flow Cytometry, Melanoma, Experimental, Mice, Inbred C57BL, Neoplasm Transplantation, Phagocytosis, Skin Neoplasms, Transfection, Blotting, Western, Genetic Therapy, Gene Expression, B7-1 Antigen, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, RNA, Small Interfering, Nanotubes, Carbon, Suppressor of Cytokine Signaling Proteins, Mice
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