The woodchuck hepatitis virus post-transcriptional regulatory element reduces readthrough transcription from retroviral vectors

In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector

Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996) · Science · doi:10.1126/science.272.5259.263 · Cited by 4,903

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of Transgenes Delivered by Retroviral Vectors

Zufferey, R., Donello, J. E., Trono, D., & Hope, T. J. (1999) · Journal of Virology · doi:10.1128/jvi.73.4.2886-2892.1999 · Cited by 1,030

A transient three-plasmid expression system for the production of high titer retroviral vectors

Soneoka, Y., Cannon, P. M., Ramsdale, E. E., Griffiths, J. C., Romano, G., Kingsman, S. M., & Kingsman, A. J. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.4.628 · Cited by 686

Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element

Donello, J. E., Loeb, J. E., & Hope, T. J. (1998) · Journal of Virology · doi:10.1128/jvi.72.6.5085-5092.1998 · Cited by 350

Enhanced Expression of Transgenes from Adeno-Associated Virus Vectors with the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element: Implications for Gene Therapy

Loeb, J. E., Cordier, W. S., Harris, M. E., Weitzman, M. D., & Hope, T. J. (1999) · Human Gene Therapy · doi:10.1089/10430349950016942 · Cited by 279

Inefficient processing impairs release of RNA from the site of transcription

Custódio, N., Carmo‐Fonseca, M., Geraghty, F., Periera, H. S., Grosveld, F. G., & Antoniou, M. N. (1999) · The EMBO Journal · doi:10.1093/emboj/18.10.2855 · Cited by 220

Context Dependence of Different Modules for Posttranscriptional Enhancement of Gene Expression from Retroviral Vectors

Schambach, A., Wodrich, H., Hildinger, M., Bohne, J., Kräusslich, H.-G., & Baum, C. (2000) · Molecular Therapy · doi:10.1006/mthe.2000.0191 · Cited by 194

Cells expressing human glucocerebrosidase from a retroviral vector repopulate macrophages and central nervous system microglia after murine bone marrow transplantation

Krall, W. J., Challita, P. M., Perlmutter, L., Skelton, D. C., & Kohn, D. B. (1994) · Blood · doi:10.1182/blood.v83.9.2737.2737 · Cited by 163

Role of the Hepatitis B Virus Posttranscriptional Regulatory Element in Export of Intronless Transcripts

Huang, Z., & Yen, T. S. B. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.7.3864 · Cited by 154

Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression

Schambach, A., Bohne, J., Baum, C., Hermann, F. G., Egerer, L., von Laer, D., & Giroglou, T. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302698 · Cited by 145

Impact of Aging on the Regenerative Properties of Bone Marrow-, Muscle-, and Adipose-Derived Mesenchymal Stem/Stromal Cells

Beane, O. S., Fonseca, V. C., Cooper, L. L., Koren, G., & Darling, E. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0115963 · Cited by 325

Lentiviral Vectors to Probe and Manipulate the Wnt Signaling Pathway

Fuerer, C., & Nusse, R. (2010) · PLoS ONE · doi:10.1371/journal.pone.0009370 · Cited by 283

Biosafety Features of Lentiviral Vectors

Schambach, A., Zychlinski, D., Ehrnstroem, B., & Baum, C. (2013) · Human Gene Therapy · doi:10.1089/hum.2012.229 · Cited by 173

Viral Vectors: The Road to Reducing Genotoxicity

David, R. M., & Doherty, A. (2016) · Toxicological Sciences · doi:10.1093/toxsci/kfw220 · Cited by 176

State-of-the-Art Lentiviral Vectors for Research Use: Risk Assessment and Biosafety Recommendations

Pauwels, K., Gijsbers, R., Toelen, J., Schambach, A., Willard-Gallo, K., Verheust, C., Debyser, Z., & Herman, P. (2009) · Current Gene Therapy · doi:10.2174/156652309790031120 · Cited by 106

New developments in lentiviral vector design, production and purification

Segura, M. M., Mangion, M., Gaillet, B., & Garnier, A. (2013) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2013.779249 · Cited by 127

Designing Lentiviral Vectors for Gene Therapy of Genetic Diseases

Poletti, V., & Mavilio, F. (2021) · Viruses · doi:10.3390/v13081526 · Cited by 111

CAR-T cell potency: from structural elements to vector backbone components

Mazinani, M., & Rahbarizadeh, F. (2022) · Biomarker Research · doi:10.1186/s40364-022-00417-w · Cited by 111

The Use of Chromatin Insulators to Improve the Expression and Safety of Integrating Gene Transfer Vectors

Emery, D. W. (2011) · Human Gene Therapy · doi:10.1089/hum.2010.233 · Cited by 98

Validation of a mutated PRE sequence allowing high and sustained transgene expression while abrogating WHV-X protein synthesis: application to the gene therapy of WAS

Zanta, M.-A., Charrier, S., Brice-Ouzet, A., Martin, S., Opolon, P., Thrasher, A. J., Hope, T. J., & Galy, A. H. M. (2009) · Gene Therapy · doi:10.1038/gt.2009.3 · Cited by 89

Efficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities

Lin, P. Y., Lin, Y., Lennon, D. P., Correa, D., SCHLUCHTER, M. D., & Caplan, A. I. (2012) · Stem Cells Translational Medicine · doi:10.5966/sctm.2012-0086 · Cited by 83

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery

Galla, M., Schambach, A., Falk, C. S., Maetzig, T., Kuehle, J., Lange, K., Zychlinski, D., Heinz, N., Brugman, M. H., Göhring, G., et al. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr384 · Cited by 75

Use of HIV as a gene transfer vector.

Pluta, K. D., & Kacprzak, M. M. (2009) · Acta Biochimica Polonica · doi:10.18388/abp.2009_2490 · Cited by 76

Mechanism of Reduction in Titers From Lentivirus Vectors Carrying Large Inserts in the 3′LTR

Urbinati, F., Arumugam, P. I., Higashimoto, T., Perumbeti, A., Mitts, K., Xia, P., & Malik, P. (2009) · Molecular Therapy · doi:10.1038/mt.2009.89 · Cited by 69

Adeno-Associated Viral Gene Therapy for Inherited Retinal Disease

Ong, T., Pennesi, M. E., Birch, D. G., Lam, B. L., & Tsang, S. H. (2019) · Pharmaceutical Research · doi:10.1007/s11095-018-2564-5 · Cited by 66

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery

Galla, M., Schambach, A., Falk, C. S., Maetzig, T., Kuehle, J., Lange, K., Zychlinski, D., Heinz, N., Brugman, M. H., Göhring, G., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr1266 · Cited by 59

Inclusion of the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances AAV2-Driven Transduction of Mouse and Human Retina

Patrício, M. I., Barnard, A. R., Orlans, H. O., McClements, M. E., & MacLaren, R. E. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2016.12.006 · Cited by 59

Transient Retrovirus-Based CRISPR/Cas9 All-in-One Particles for Efficient, Targeted Gene Knockout

Knopp, Y., Geis, F. K., Heckl, D., Horn, S., Neumann, T., Kuehle, J., Meyer, J., Fehse, B., Baum, C., Morgan, M. A., et al. (2018) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2018.09.006 · Cited by 63

Adeno-Associated Virus-Mediated Rhodopsin Replacement Provides Therapeutic Benefit in Mice with a Targeted Disruption of the Rhodopsin Gene

Palfi, A., Millington‐Ward, S., Chadderton, N., O’Reilly, M., Goldmann, T., Humphries, M. M., Li, T., Wolfrum, U., Humphries, P., Kenna, P. F., et al. (2009) · Human Gene Therapy · doi:10.1089/hum.2009.119 · Cited by 56

Designing and optimizing AAV-mediated gene therapy for neurodegenerative diseases: from bench to bedside

Xu, L., Yao, S., Ding, Y. E., Xie, M., Feng, D., Sha, P., Tan, L., Bei, F., & Yao, Y. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05661-2 · Cited by 48

Current landscape of vector safety and genotoxicity after hematopoietic stem or immune cell gene therapy

Ottaviano, G., & Qasim, W. (2025) · Leukemia · doi:10.1038/s41375-025-02585-8 · Cited by 45

Genetic Therapy for Beta-Thalassemia: From the Bench to the Bedside

Arumugam, P. I., & Malik, P. (2010) · Hematology · doi:10.1182/asheducation-2010.1.445 · Cited by 41

Genetic modification of lymphocytes by retrovirus-based vectors

Suerth, J. D., Schambach, A., & Baum, C. (2012) · Current Opinion in Immunology · doi:10.1016/j.coi.2012.08.007 · Cited by 38

High-efficiency Transient Transduction of Human Embryonic Stem Cell–derived Neurons With Baculoviral Vectors

Zeng, J., Du, J., Lin, J., Bak, X. Y., Wu, C., & Wang, S. (2009) · Molecular Therapy · doi:10.1038/mt.2009.124 · Cited by 38

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Kasala, D., Yoon, A. R., Hong, J., Kim, S. W., & Yun, C. (2016) · Nanomedicine · doi:10.2217/nnm-2016-0060 · Cited by 38

Pre-clinical development of gene modification of haematopoietic stem cells with chimeric antigen receptors for cancer immunotherapy

Larson, S. M., Truscott, L. C., Chiou, T., Patel, A., Kao, R. L., Tu, A. A., Tyagi, T., Lü, X., Elashoff, D. A., & Oliveira, S. N. D. (2017) · Human Vaccines & Immunotherapeutics · doi:10.1080/21645515.2016.1268745 · Cited by 32

Strategies to improve safety profile of AAV vectors

Suoranta, T., Laham-Karam, N., & Ylä‐Herttuala, S. (2022) · Frontiers in Molecular Medicine · doi:10.3389/fmmed.2022.1054069 · Cited by 32

The transformative potential of HSC gene therapy as a genetic medicine

Sagoo, P., & Gaspar, H. B. (2021) · Gene Therapy · doi:10.1038/s41434-021-00261-x · Cited by 27

Neonatal Gene Therapy of Glycogen Storage Disease Type Ia Using a Feline Immunodeficiency Virus–based Vector

Grinshpun, A., Condiotti, R., Waddington, S. N., Peer, M., Zeig, E., Peretz, S., Simerzin, A., CHOU, J. Y., Pan, C., Giladi, H., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.119 · Cited by 29

Sustained Expression from DNA Vectors

Wong, S. P., Argyros, O., & Harbottle, R. P. (2014) · Advances in genetics · doi:10.1016/bs.adgen.2014.11.002 · Cited by 26

The design of retroviral vectors used in the CAR‐T products, risk management, and future perspective

Yin, H., & Wei, X. (2025) · MedComm · doi:10.1002/mco2.70067 · Cited by 27

Amelioration of muscle and nerve pathology of Lama2-related dystrophy by AAV9-laminin-αLN linker protein

McKee, K. K., & Yurchenco, P. D. (2022) · JCI Insight · doi:10.1172/jci.insight.158397 · Cited by 23

MMP14 cleaves PTH1R in the chondrocyte-derived osteoblast lineage, curbing signaling intensity for proper bone anabolism

Chu, T. L., Chen, P., Yu, A. X., Kong, M., Tan, Z., Tsang, K. Y., Zhou, Z., & Cheah, K. S. E. (2023) · eLife · doi:10.7554/elife.82142 · Cited by 20

Transcriptional targeting to brain cells: Engineering cell type-specific promoter containing cassettes for enhanced transgene expression

Boulaire, J., Balani, P., & Wang, S. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.02.007 · Cited by 22

Genetically engineered birds; pre-CRISPR and CRISPR era

Dehdilani, N., Taemeh, S. Y., Goshayeshi, L., & Dehghani, H. (2021) · Biology of Reproduction · doi:10.1093/biolre/ioab196 · Cited by 21

Advances in Recombinant Adeno-Associated Virus Vectors for Neurodegenerative Diseases

Li, L., Vasan, L., Kartono, B., Clifford, K. P., Attarpour, A., Sharma, R., Mandrozos, M., Kim, A., Zhao, W., Belotserkovsky, A., et al. (2023) · Biomedicines · doi:10.3390/biomedicines11102725 · Cited by 19

Generation of High-Titer Self-Inactivated γ-Retroviral Vector Producer Cells

Ghani, K., Boivin-Welch, M., Roy, S., Piaceski, A. D., Barbier, M. A., Pope, E., Germain, L., & Caruso, M. (2019) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2019.05.013 · Cited by 20

Prevention of transgene silencing during human pluripotent stem cell differentiation

Uenaka, T., Napole, A., Saha, A., Sun, D., Singavarapu, A., Calzada, E., Chen, J., Erlebach, L., McQuade, A., Ramos, D. M., et al. (2026) · Cell stem cell · doi:10.1016/j.stem.2026.01.007 · Cited by 21

Different Ectopic Hoxa2 Expression Levels in Mouse Cranial Neural Crest Cells Result in Distinct Craniofacial Anomalies and Homeotic Phenotypes

Kitazawa, T., Minoux, M., Ducret, S., & Rijli, F. M. (2022) · Journal of Developmental Biology · doi:10.3390/jdb10010009 · Cited by 16

Lentiviral vectors encoding human MUC1-specific, MHC-unrestricted single-chain TCR and a fusion suicide gene: potential for universal and safe cancer immunotherapy

Chen, X., Gao, W., Gambotto, A. A., & Finn, O. J. (2008) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-008-0624-0 · Cited by 15

HIV-1-Based Lentiviral Vectors

Liu, Y. P., & Berkhout, B. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-670-2_22 · Cited by 15

Lentiviral Vectors: From Wild-Type Viruses to Efficient Multi-Functional Delivery Vectors

Arrasate, A., Lopez-Robles, C., Zuazo, M., Baños-Mateos, S., Martín, C., Lamsfus‐Calle, A., & Fertin, M. J. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26178497 · Cited by 14

Elements of lentiviral vector design toward gene therapy for treating mucopolysaccharidosis I

Ou, L., Przybilla, M. J., Koniar, B. L., & Whitley, C. B. (2016) · Molecular Genetics and Metabolism Reports · doi:10.1016/j.ymgmr.2015.11.004 · Cited by 15

Develop an efficient and specific AAV-based labeling system for Muller glia in mice

Gao, Y., Fang, K., Yan, Z., Zhang, H., Geng, G., Wu, W., Ding, X., Zhang, H., Zhong, N., Wang, Q., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-27013-0 · Cited by 13

Improved microRNA suppression by WPRE-linked tough decoy microRNA sponges

Hollensen, A. K., Thomsen, R., Bak, R. O., Petersen, C. C., Ermegaard, E. R., Aagaard, L., Damgaard, C. K., & Mikkelsen, J. G. (2017) · RNA · doi:10.1261/rna.061192.117 · Cited by 15

Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

Galla, M., Schambach, A., & Baum, C. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_10 · Cited by 13

ExportAid: database of RNA elements regulating nuclear RNA export in mammals

Giulietti, M., Milantoni, S. A., Armeni, T., Principato, G., & Piva, F. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu620 · Cited by 12

Sustained expression of coagulation factor IX by modified cord blood‐derived mesenchymal stromal cells

Dodd, M., Marquez‐Curtis, L. A., Janowska‐Wieczorek, A., & Hortelano, G. (2014) · The Journal of Gene Medicine · doi:10.1002/jgm.2769 · Cited by 11

Comparison of Lentiviral Packaging Mixes and Producer Cell Lines for RNAi Applications

Albrecht, C. H., Hosiner, S., Tichy, B., Aldrian, S. M., Hajdú, Š., & Nürnberger, S. (2015) · Molecular Biotechnology · doi:10.1007/s12033-015-9843-8 · Cited by 10

Modeling of congenital erythropoietic porphyria by RNA interference: a new tool for preclinical gene therapy evaluation

Richard, É., Lalanne, M., Lamrissi‐Garcia, I., Guyonnet‐Dupérat, V., Richard, E., Pitard, V., Mazurier, F., Moreau‐Gaudry, F., Ged, C., & de Verneuil, H. (2010) · The Journal of Gene Medicine · doi:10.1002/jgm.1478 · Cited by 10

A pathological phosphorylation pattern enhances tau cooperativity on microtubules and facilitates tau filament assembly

Fan, X., Truong, J. B., Okada, K., Lin, H., Ori-McKenney, K. M., & McKenney, R. J. (2026) · Communications Biology · doi:10.1038/s42003-026-10797-6 · Cited by 10

The Post-Transcriptional Regulatory Element of Hepatitis B Virus: From Discovery to Therapy

Mouzannar, K., Schauer, A., & Liang, T. J. (2024) · Viruses · doi:10.3390/v16040528 · Cited by 8

Tracing goes viral: Viruses that introduce expression of fluorescent proteins in chemically-specific neurons

Luchicchi, A., Pattij, T., Viaña, J. N. M., de Kloet, S. F., & Marchant, N. J. (2020) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2020.109004 · Cited by 8

Neural development in human embryonic stem cells-applications of lentiviral vectors

Dottori, M., Tay, C. Y., & Hughes, S. M. (2011) · Journal of Cellular Biochemistry · doi:10.1002/jcb.23116 · Cited by 7

Lentiviral vectors with amplified β cell-specific gene expression

Shaw, K. L., Pais, E., Ge, S., Hardee, C. L., Skelton, D. C., Hollis, R. P., Crooks, G. M., & Kohn, D. B. (2009) · Gene Therapy · doi:10.1038/gt.2009.49 · Cited by 7

Enhancement of transcription efficiency by TAR-Tat system increases the functional expression of human olfactory receptors

Ojiro, I., Katsuyama, H., Kaneko, R., Ogasawara, S., Murata, T., Terada, Y., & Ito, K. (2024) · PLoS ONE · doi:10.1371/journal.pone.0306029 · Cited by 6

Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry

Wright, J. H., Huang, L.-Y., Weaver, S., Archila, L. D., McAfee, M. S., Hirayama, A. V. V., Chapuis, A. G., Bleakley, M., Rongvaux, A., Turtle, C. J., et al. (2020) · Journal of Immunological Methods · doi:10.1016/j.jim.2020.112955 · Cited by 6

Woodchuck hepatitis post-transcriptional regulatory element improves transgene expression and stability mediated by episomal vectors in CHO-K1 cells

Wang, X.-Y., Zhang, W., Jia, Y., Wang, M., Yi, D., & Wang, T. (2020) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmaa105 · Cited by 6

Spectrum, pathobiology, mechanistic insights and diagnostic challenges of post-CAR T cell therapy lymphoproliferative disorders

Ali, A. M., Özdemirli, M., Hamilton, M. P., & Jain, M. D. (2026) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-026-01147-w · Cited by 7

Retroviral Vectors for Cell and Gene Therapy

Schambach, A., Maetzig, T., & Baum, C. (2008) · CRC Press eBooks · doi:10.1201/9780849387999.pt1 · Cited by 5

Plugging synthetic DNA nanoparticles into the central dogma of life

Neyra, K., Desai, S., & Mathur, D. (2024) · Chemical Communications · doi:10.1039/d4cc04648j · Cited by 4

Neuron-specific WWOX gene therapy produces dose-dependent, durable rescue in a model of WWOX-related epileptic encephalopathy

Obeid, M., Akkawi, R., Repudi, S., Singh, P. K., Abudiab, B., Jebara, T., Berent, A., Brennan, T., Weiss, Y., Shekh‐Ahmad, T., et al. (2026) · Molecular Therapy Advances · doi:10.1016/j.omta.2026.201791 · Cited by 4

Laterally spreading AAV.SPR enables safe and efficient RS1 delivery to the macula after peripheral subretinal injection

Andrés‐Mateos, E., Kozlowski, C., Zhang, H., Fajardo, D., Xu, E. M., Navas‐Enamorado, I., Calabro, K. R., Rayaprolu, S., Havlik, L. P., Handy, T. E., et al. (2026) · Molecular Therapy · doi:10.1016/j.ymthe.2026.03.014 · Cited by 4

Adenovirus 5 Vectors Expressing SARS-CoV-2 Proteins

Bachus, S., Akkerman, N., Fulham, L., Graves, D., Stephanson, C., Memon, H., Miller, M. S., & Pelka, P. (2022) · mSphere · doi:10.1128/msphere.00998-21 · Cited by 3

The cHS4 Chromatin Insulator Reduces the Rate of Retroviral Vector-Mediated Gene Dysregulation Associated with Aberrant Vector Transcription

Zhou, X., Liu, Q., Wang, D., Zhang, X., Emery, D. W., & Li, C. L. (2017) · Cytogenetic and Genome Research · doi:10.1159/000464315 · Cited by 3

Gene Therapy Vectors

Bauer, G., & Anderson, J. S. (2014) · SpringerBriefs in biochemistry and molecular biology · doi:10.1007/978-1-4939-0434-1_4 · Cited by 3

Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout

Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B. R., Sahasrabudhe, A., Ahfeldt, T., & Blanchard, J. (2025) · iScience · doi:10.1016/j.isci.2025.112816 · Cited by 4

Humanized extracellular vesicles for efficient RNA delivery

Ma, X., Zhao, S. R., & Cepko, C. L. (2026) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2525726123 · Cited by 2

Sub-genomic flaviviral RNA elements increase the stability and abundance of recombinant AAV vector transcripts

Meganck, R. M., Ogurlu, R., Liu, J., Møller-Tank, S., Tse, L. V., Blondel, L. O., Rosales, A., Hall, A. C., Vincent, H. A., Moorman, N. J., et al. (2024) · Journal of Virology · doi:10.1128/jvi.00095-24 · Cited by 2

An adjusted droplet digital PCR assay for quantification of vector copy number in CAR-T cell and TCR-T cell products

Ma, J., Meyer, S., Olweus, J., Jin, C., & Yu, D. (2024) · Immuno-Oncology Technology · doi:10.1016/j.iotech.2024.101031 · Cited by 2

Engineering fluorescent reporters in human pluripotent stem cells and strategies for live imaging human neurogenesis

Dady, A., Davidson, L., Loyer, N., Rappich, S., Findlay, G. M., Sanders, T., Januschke, J., & Storey, K. G. (2025) · Development · doi:10.1242/dev.205082 · Cited by 2

Adeno-Associated Virus Vector Design–Moving the Adeno-Associated Virus to a Bioengineered Therapeutic Nanoparticle

Jäschke, N. M., & Büning, H. (2022) · Hematology/Oncology Clinics of North America · doi:10.1016/j.hoc.2022.04.002 · Cited by 2

Insertional Mutagenesis in Hematopoietic Cells: Lessons Learned from Adverse Events in Clinical Gene Therapy Trials

Müller, L., Milsom, M. D., & Williams, D. A. (2010) · Springer eBooks · doi:10.1007/978-1-4419-7656-7_6 · Cited by 2

Forensic Analysis of Novel SARS2r-CoV Identified in Game Animal Datasets in China Shows Evolutionary Relationship to Pangolin GX CoV Clade and Apparent Genetic Experimentation

Jones, A., Massey, S. E., Zhang, D., Deigin, Y., & Quay, S. C. (2022) · Applied Microbiology · doi:10.3390/applmicrobiol2040068 · Cited by 2

Retroviral Integration Target Site Selection

Ciuffi, A., & Bushman, F. D. (2011) · Wiley eBooks · doi:10.1002/9781118015377.ch5 · Cited by 2

Development of viral & non-viral episomal vectors for gene therapy applications

Kymäläinen, H. (2012) · OpenGrey (Institut de l'Information Scientifique et Technique) · Cited by 2

qPCR assay for detection of Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Elements from CAR-T and TCR-T cells in fresh and formalin-fixed tissue

Pullarkat, S., Black, G., Bleakley, M., Buenrostro, D., Chapuis, A. G., Hirayama, A. V. V., Jaeger-Ruckstuhl, C. A., Kimble, E. L., Lee, B. M., Maloney, D. G., et al. (2024) · PLoS ONE · doi:10.1371/journal.pone.0303057 · Cited by 1

Enhancing human ACE2 expression in mouse models to improve COVID ‐19 research

Jiaoyang, S., Shaofei, C., Guangliang, H., Xiongzhi, Q., Haofeng, L., Rui, M., Grillari, J., Zheng‐Li, S., Jiekai, C., Meiqin, L., et al. (2024) · FEBS Open Bio · doi:10.1002/2211-5463.13934 · Cited by 1

Systematic evaluation of transposon vector elements to establish high-producing stable CHO cell pools achieving > 10 g/L monoclonal antibody titers

Tsachaki, M., Peltret, M., Laplace, T. B., Vaxelaire-Hily, E., Mosbaoui, F., Aillerie, C., Schmid, A., Galea, A., Contarin, F., Duarte, L., et al. (2026) · New Biotechnology · doi:10.1016/j.nbt.2026.03.008 · Cited by 1

Structural basis of lipid-dependent allosteric gating mechanisms for PC1-PC2 ion channel

Chen, M., Wang, Z., Shi, Y., Huang, G., Morais, T. D. C., Pillai, A., Wang, Y., Mou, M. A., Jing, D., Fan, R., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-75084-8 · Cited by 1

Engineering fluorescent reporters in human pluripotent cells and strategies for live imaging human neurogenesisPreprint

Dady, A., Davidson, L., Loyer, N., Rappich, S., Findlay, G. M., Sanders, T., Januschke, J., & Storey, K. G. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.05.01.591467 · Cited by 1

Transcription, Translation, and the Control of Gene Expression in Mammalian Cells

Mullick, A., & Massie, B. (2010) · Wiley eBooks · doi:10.1002/9780470054581.eib565

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Sun, Y. (2014) · Digital Commons-TMC (Texas Medical Center)

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Tomita, H., & Sugano, E. (2017) · Cambridge University Press eBooks · doi:10.1017/9781107281875.028

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Ong, J. M., Brown, C. R., Mendel, M. C., & Cost, G. J. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/126904

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Sweeney, N. P. (2015) · Spiral (Imperial College London) · doi:10.25560/50704

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Okan, I. C. T., Ahmadian, M., Tutuncu, Y., Altay, H. Y., & Ağca, C. (2021) · Journal of Visualized Experiments · doi:10.3791/63038

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Jones, A., Massey, S. E., Zhang, D., Deigin, Y., & Quay, S. C. (2022) · Research Square · doi:10.21203/rs.3.rs-1836803/v1

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Jones, A., Massey, S. E., Zhang, D., Deigin, Y., & Quay, S. C. (2022) · Research Square · doi:10.21203/rs.3.rs-1836803/v2

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Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B. R., Sahasrabudhe, A., Ahfeldt, T., & Blanchard, J. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4886465

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Jin, H., Cho, Y. R., & Jung, Y. T. (2025) · Acta Virologica · doi:10.3389/av.2025.14262

Gamma-retrovirus design for efficacious microRNA-mediated gene knock-down and protein co-expression without compromising titers

de Silly, R. V., Seijo, B., Maillard, J., Reichenbach, P., & Irving, M. B. (2025) · NAR Molecular Medicine · doi:10.1093/narmme/ugaf030

NATO3 protects dopaminergic neurons in mouse in vivo and human in vitro Parkinson’s disease modelsPreprint

Valencia-Alarcón, E. P., Duret, L. C., Kobayashi, E., Cattaneo, O., Schneider, B. L., & Nagoshi, E. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.10.681573

Investigation of the effects of virus integration on host gene expression in mouse tumour samples

Osejindu, E. (2011) · Brunel University Research Archive (BURA) (Brunel University London)

Enhanced transcriptional insulation of lentiviral vectors using “sequence-upgraded polyA long terminal repeats” or “supA-LTRs”

Wright, J., Alberts, B. M., Quiles, M. L. M., Guarino, A., Chipchase, D., Clarkson, N. G., Mitrophanous, K. A., & Farley, D. C. (2025) · Molecular Therapy Advances · doi:10.1016/j.omta.2025.201654

AAV-TNNI3 rescues an experimental murine Tnni3 mutation resulting in thin filament mediated DCM

Bushway, P., Feng, W., Cruz, M. S., Maisel, S., Shathaya, A., Rao, P., Patel, U., Park, J., Chen, C., Tang, Z., et al. (2026) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2026.01.001

Optimisation de la stratégie de thérapie génique par vecteur lentiviral pour la β-thalassémie

Giorgi, M. (2019) · theses.fr (ABES) · doi:10.70675/041f272dz23faz452bzb352z46cf95acb76d

Lentiviral-mediated expression of cytochrome P450 2D6 in HepaRG cells: new means for in vitro xenobiotic biotransformation and cellular response to chimeric viral mRNA

Coppens-Exandier, H., Mackanga, F., Darde, T., Bonhomme, C., Angenard, G., Leroyer, P., Ribault, C., Gicquel, T., Pelletier, R., Roshchina, F., et al. (2026) · Archives of Toxicology · doi:10.1007/s00204-026-04453-5

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Goberdhan, S. C., Czubala, M. A., Thomas, S. E., Taylor, P. R., & Connor‐Robson, N. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.23.727402

Polymerizing laminins: Assembly, functions and disorders

Yurchenco, P. D. (2026) · Matrix Biology · doi:10.1016/j.matbio.2026.102031

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of Transgenes Delivered by Retroviral Vectors

Zufferey, R., Donello, J. E., Trono, D., & Hope, T. J. (1999) · Journal of Virology · doi:10.1128/jvi.73.4.2886-2892.1999 · Cited by 1,030

775. The Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element Reduces Readthrough Transcription from Retroviral Vectors To Improve Vector Titers and Expression

(2007) · Molecular Therapy · doi:10.1016/s1525-0016(16)44981-6

The hepatitis B virus posttranscriptional regulatory element is composed of two subelements

Zufferey, R., Donello, J. E., Trono, D., & Hope, T. J. (1996)

Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression

Schambach, A., Bohne, J., Baum, C., Hermann, F. G., Egerer, L., von Laer, D., & Giroglou, T. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302698 · Cited by 145

Effect of posttranscriptional regulatory elements on transgene expression and virus production in the context of retrovirus vectors

Hlavatý, J., Schittmayer, M., Stracke, A., Jandl, G., Knapp, E., Felber, B. K., Salmons, B., Günzburg, W. H., & Renner, M. (2005) · Virology · doi:10.1016/j.virol.2005.06.037 · Cited by 33