The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.
Molecular signature of CD34+ hematopoietic stem and progenitor cells of patients with CML in chronic phase
Abstract
In this study, we provide a molecular signature of highly enriched CD34+ cells from bone marrow of untreated patients with chronic myelogenous leukemia (CML) in chronic phase in comparison with normal CD34+ cells using microarrays covering 8746 genes. Expression data reflected several BCR-ABL-induced effects in primary CML progenitors, such as transcriptional activation of the classical mitogen-activated protein kinase pathway and the phosphoinositide-3 kinase/AKT pathway as well as downregulation of the proapoptotic gene IRF8. Moreover, novel transcriptional changes in comparison with normal CD34+ cells were identified. These include upregulation of genes involved in the transforming growth factorbeta pathway, fetal hemoglobin genes, leptin receptor, sorcin, tissue inhibitor of metalloproteinase 1, the neuroepithelial cell transforming gene 1 and downregulation of selenoprotein P. Additionally, genes associated with early hematopoietic stem cells (HSC) and leukemogenesis such as HoxA9 and MEIS1 were transcriptionally activated. Differential expression of differentiation-associated genes suggested an altered composition of the CD34+ cell population in CML. This was confirmed by subset analyses of chronic phase CML CD34+ cells showing an increase of the proportion of megakaryocyte-erythroid progenitors, whereas the proportion of HSC and granulocyte-macrophage progenitors was decreased in CML. In conclusion, our results give novel insights into the biology of CML and could provide the basis for identification of new therapeutic targets.
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“Gene set enrichment analysis showed that genes on AML-specific loops were enriched in hematopoiesis and myeloid transformation pathways28,29 (Extended Data Fig. 4c).”
From Subtype-specific 3D genome alteration in acute myeloid leukaemia (2022) · cites this paper as [29]
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Cites
In silico prediction of protein-protein interactions in human macrophages
Souiai, O., Guerfali, F. Z., Miled, S. B., Brun, C., & Benkahla, A. (2014) · BMC Research Notes · doi:10.1186/1756-0500-7-157 · Cited by 10,785
Significance analysis of microarrays applied to the ionizing radiation response
Tusher, V. G., Tibshirani, R., & Chu, G. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.091062498 · Cited by 10,568
Minimum information about a microarray experiment (MIAME)-toward standards for microarray data
Brazma, A., Hingamp, P., Quackenbush, J., Sherlock, G., Spellman, P., Stoeckert, C., Aach, J., Ansorge, W., Ball, C. A., Causton, H. C., et al. (2001) · Nature Genetics · doi:10.1038/ng1201-365 · Cited by 4,109
Huber, W., von Heydebreck, A., Sültmann, H., Poustka, A., & Vingron, M. (2002) · Bioinformatics · doi:10.1093/bioinformatics/18.suppl_1.s96 · Cited by 2,440
Daley, G. Q., Etten, R. A. V., & Baltimore, D. (1990) · Science · doi:10.1126/science.2406902 · Cited by 2,141
The molecular biology of chronic myeloid leukemia
Deininger, M. W., Goldman, J. M., & Melo, J. V. (2000) · Blood · doi:10.1182/blood.v96.10.3343 · Cited by 1,654
The Biology of Chronic Myeloid Leukemia
Faderl, S. H., Talpaz, M., Estrov, Z. E., O'Brien, S. M., Kurzrock, R., & Kantarjian, H. M. (1999) · New England Journal of Medicine · doi:10.1056/nejm199907153410306 · Cited by 1,322
Intracellular signalling pathways activated by leptin
Frühbeck, G. (2005) · Biochemical Journal · doi:10.1042/bj20051578 · Cited by 851
Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton
Tapon, N. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80156-1 · Cited by 819
Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells
Gainsford, T., Willson, T. A., Metcalf, D., Handman, E., McFarlane, C., Ng, A. P., Nicola, N. A., Alexander, W. S., & Hilton, D. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.25.14564 · Cited by 758
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The IRF Family Transcription Factors in Immunity and Oncogenesis
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Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow
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Metadata
- Authors
- Diaz-Blanco, E., Bruns, I., Neumann, F., Fischer, J. C., Graef, T., Rosskopf, M., Brors, B., Pechtel, S., Bork, S., Koch, A., Baer, A., Rohr, U.-P., Kobbe, G., Haeseler, A. V., Gattermann, N., Haas, R., & Kronenwett, R.
- Affiliations
- Heinrich Heine University Düsseldorf (DE)
- German Cancer Research Center (DE)
- University of Vienna (AT)
- Corresponding author
- Kronenwett, R
- ORCID
- Venue
- Leukemia
- Published
- 2007-01-25
- Volume
- 21
- Issue
- 3
- Pages
- 494-504
- ISSN
- 0887-6924 (ISSN-L)
- 1476-5551
- OpenAlex
- W2564723484
- Cited by
- 148
- References
- 78
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 5 · 2022: 4 · 2021: 10 · 2020: 6 · 2019: 6 · 2018: 2 · 2017: 8 · 2016: 4 · 2015: 10 · 2014: 5 · 2013: 12 · 2012: 7
- FWCI
- 9.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Leukemia (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Chronic Myeloid Leukemia Treatments
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Keywords
- chronic myeloid leukemia, chronic phase, CD34+ hematopoietic stem and progenitor cells, BCR-ABL, gene expression signatures, MAPK signaling, phosphoinositide 3-kinase/Akt pathway, IRF8, HOXA9, MEIS1, megakaryocyte-erythroid progenitors, CFU-GM, fetal hemoglobin, selenoprotein P, leukemogenesis, therapeutic target
- MeSH terms
- Cell Adhesion, Cell Differentiation, Cell Division, DNA, Neoplasm, Hematopoietic Stem Cells, Humans, Neoplasm Proteins, Receptors, Cell Surface, RNA, Messenger, RNA, Neoplasm, Neoplastic Stem Cells, Signal Transduction, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Leukemia, Myeloid, Chronic-Phase, Up-Regulation, Gene Expression Regulation, Leukemic, Fusion Proteins, bcr-abl, Apoptosis, Receptors, Growth Factor, DNA, Complementary, Antigens, CD34, Gene Expression Profiling, Intercellular Signaling Peptides and Proteins, Receptors, Leptin
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