Cited by 80Skin Protection and AgingDNA Repair MechanismsCancer-related Molecular Pathways

G2 phase cell cycle arrest in human skin following UV irradiation

Pavey, Sandra, Russell, Terry, Gabrielli, Brian·2001·Oncogene

Abstract

The contribution of the short wavelength ultraviolet (UV) component of sunlight to the aetiology of skin cancer has been widely acknowledged, although its direct contribution to tumour initiation or progression is still poorly understood. The loss of normal cell cycle controls, particularly checkpoint controls, are a common feature of cancer. UV radiation causes both G1 and G2 phase checkpoint arrest in vitro cultured cells. In this study we have investigated the cell cycle responses to suberythemal doses of UV on skin. We have utilized short-term whole organ skin cultures, and multi parameter immunohistochemical and biochemical analysis to demonstrate that basal and suprabasal layer melanocytes and keratinocytes undergo a G2 phase cell cycle arrest for up to 48 h following irradiation. The arrest is associated with increased p16 expression but no apparent p53 involvement. This type of organ culture provides a very useful model system, combining the ease of in vitro manipulation with the ability to perform detailed molecular analysis in a normal tissue environment.

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Crossref · 10.1038/sj.onc.1204707

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Authors
Pavey, S., Russell, T., & Gabrielli, B.
Affiliations
The University of Queensland (AU)
Corresponding author
Gabrielli, Brian
Venue
Oncogene
Published
2001-09-27
Volume
20
Issue
43
Pages
6103-6110
ISSN
  • 0950-9232 (ISSN-L)
  • 1476-5594
OpenAlex
W1993612200
Cited by
80
References
37
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 1
FWCI
6.63
Type
journal article
Language
en
Open access
closed
Locations
  • Oncogene (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Australian Research Council
  • National Health and Medical Research Council
  • National Medical Research Council
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Keywords
G2 cell cycle arrest, ultraviolet radiation, melanocytes, keratinocytes, p16, p53, cell cycle checkpoints
MeSH terms
Bromodeoxyuridine, Cell Cycle, Cells, Cultured, Humans, Immunohistochemistry, Male, Organ Culture Techniques, Precipitin Tests, Skin, Time Factors, Ultraviolet Rays, Immunoblotting, Up-Regulation, Tumor Suppressor Protein p53, G2 Phase, CDC2 Protein Kinase, Phosphotyrosine, Cyclin B, Cyclin-Dependent Kinase Inhibitor p16, Cyclin B1

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