G2 phase cell cycle arrest in human skin following UV irradiation
Abstract
The contribution of the short wavelength ultraviolet (UV) component of sunlight to the aetiology of skin cancer has been widely acknowledged, although its direct contribution to tumour initiation or progression is still poorly understood. The loss of normal cell cycle controls, particularly checkpoint controls, are a common feature of cancer. UV radiation causes both G1 and G2 phase checkpoint arrest in vitro cultured cells. In this study we have investigated the cell cycle responses to suberythemal doses of UV on skin. We have utilized short-term whole organ skin cultures, and multi parameter immunohistochemical and biochemical analysis to demonstrate that basal and suprabasal layer melanocytes and keratinocytes undergo a G2 phase cell cycle arrest for up to 48 h following irradiation. The arrest is associated with increased p16 expression but no apparent p53 involvement. This type of organ culture provides a very useful model system, combining the ease of in vitro manipulation with the ability to perform detailed molecular analysis in a normal tissue environment.
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UV radiation induces both G1 and G2 cell cycle arrest, in a p53-dependent or independent pathway, respectively.
“As shown by previous research, UV radiation induces both G1 and G2 cell cycle arrest (in a p53-dependent or independent pathway, respectively) [72].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [72]
UVR can cause cell cycle arrest both at G1 and G2 phases of cell cycle.
“Previous studies have shown that UVR can cause cell cycle arrest both at G1 [63] and G2 phases [64] of cell cycle.”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [64]
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- Authors
- Pavey, S., Russell, T., & Gabrielli, B.
- Affiliations
- The University of Queensland (AU)
- Corresponding author
- Gabrielli, Brian
- Venue
- Oncogene
- Published
- 2001-09-27
- Volume
- 20
- Issue
- 43
- Pages
- 6103-6110
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W1993612200
- Cited by
- 80
- References
- 37
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 6.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Australian Research Council
- National Health and Medical Research Council
- National Medical Research Council
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- G2 cell cycle arrest, ultraviolet radiation, melanocytes, keratinocytes, p16, p53, cell cycle checkpoints
- MeSH terms
- Bromodeoxyuridine, Cell Cycle, Cells, Cultured, Humans, Immunohistochemistry, Male, Organ Culture Techniques, Precipitin Tests, Skin, Time Factors, Ultraviolet Rays, Immunoblotting, Up-Regulation, Tumor Suppressor Protein p53, G2 Phase, CDC2 Protein Kinase, Phosphotyrosine, Cyclin B, Cyclin-Dependent Kinase Inhibitor p16, Cyclin B1
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