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Cited by 25Hair Growth and DisordersSkin Protection and AgingSkin and Cellular Biology Research

Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids

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Kim, Min-Ji, Ahn, Hee-Jin, Kong, Dasom, Lee, Seunghee, Kim, Da-Hyun, Kang, Kyung-Sun·2024·Journal of Tissue Engineering
DOI 10.1177/20417314241248753PMC PMC11080775
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Abstract

Solar ultraviolet (sUV) exposure is known to cause skin damage. However, the pathological mechanisms of sUV on hair follicles have not been extensively explored. Here, we established a model of sUV-exposed skin and its appendages using human induced pluripotent stem cell-derived skin organoids with planar morphology containing hair follicles. Our model closely recapitulated several symptoms of photodamage, including skin barrier disruption, extracellular matrix degradation, and inflammatory response. Specifically, sUV induced structural damage and catagenic transition in hair follicles. As a potential therapeutic agent for hair follicles, we applied exosomes isolated from human umbilical cord blood-derived mesenchymal stem cells to sUV-exposed organoids. As a result, exosomes effectively alleviated inflammatory responses by inhibiting NF-κB activation, thereby suppressing structural damage and promoting hair follicle regeneration. Ultimately, our model provided a valuable platform to mimic skin diseases, particularly those involving hair follicles, and to evaluate the efficacy and underlying mechanisms of potential therapeutics.

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Crossref · 10.1177/20417314241248753

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  • Transient activation of WNT signalling during organoid development suppresses ectopic cartilage formation and promotes generation of large, correctly oriented skin organoids suitable for modelling solar UV-induced damage and repair.

    “Transient activation of WNT signalling during organoid development suppresses ectopic cartilage formation and promotes the generation of large, correctly oriented skin organoids suitable for modelling solar UV-induced damage and repair 16 .”

    From Vascularized human skin assembloids model compartmental skin organization and immune responsiveness in vitro (2026) · cites this paper as [16]

    The Methods (page unavailable) say a WNT agonist was added during organoid development to prevent off-target cartilage differentiation and increase organoid diameter. The Discussion (page unavailable) attributes the planar, skin-like arrangement to cutting the organoids and using an air–liquid interface, not to WNT activation alone. The paper uses those organoids to model solar UV damage and a potential treatment, but does not establish that WNT activation itself produces their orientation. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Previous studies have shown that skin organoids can generate stratified epidermal and dermal compartments, but they generally fail to establish vascular structures, a defining feature of native human skin.

    “Previous studies have shown that skin organoids can generate stratified epidermal and dermal compartments, but they generally fail to establish vascular structures, a defining feature of native human skin 13,16 .”

    From Vascularized human skin assembloids model compartmental skin organization and immune responsiveness in vitro (2026) · cites this paper as [16]

    The Results (Characterization of iPSC-derived hair-bearing SkOs; page unavailable) describe organoids with a stratified epidermis and dermis. The paper does not state that skin organoids generally fail to form vascular structures; searches for vascular structures and vascularization found no supporting passage. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Kim, M.-J., Ahn, H.-J., Kong, D., Lee, S., Kim, D.-H., & Kang, K.-S.
Affiliations
  • Adult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
  • Cytotherapy R&D Center, PRIMORIS THERAPEUTICS CO., LTD., Gwangmyeong-si, Gyeonggi-do, Republic of Korea
  • Seoul National University (KR)
  • Kangstem Biotech Co., Ltd. (South Korea) (KR)
Corresponding author
Kang, Kyung-Sun
ORCID
Venue
Journal of Tissue Engineering
Publisher
SAGE Publications
Published
2024-01
Volume
15
Pages
20417314241248753-20417314241248753
ISSN
2041-7314 (ISSN-L)
DOI
10.1177/20417314241248753
PMC
PMC11080775
OpenAlex
W4396724739
Cited by
25
References
11
Citations by year
2026: 10 · 2025: 13 · 2024: 2
FWCI
11.79
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Tissue Engineering (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Science and ICT, South Korea (23A0101L1)
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
Keywords
photodamage, hair follicle, skin organoids, skin barrier disruption, extracellular matrix degradation, inflammation, exosomes, mesenchymal stem cells, umbilical cord blood, NF-κB activation, hair follicle regeneration

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