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Cited by 81Hair Growth and DisordersTissue Engineering and Regenerative Medicinedental development and anomalies

Hair organ regeneration via the bioengineered hair follicular unit transplantation

Open access
Asakawa, Kyosuke, Toyoshima, Koh-ei, Ishibashi, Naoko, Tobe, Hirofumi, Iwadate, Ayako, Kanayama, Tatsuya, Hasegawa, Tomoko, Nakao, Kazuhisa +5 more·2012·Scientific Reports
DOI 10.1038/srep00424PMC PMC3361021
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Abstract

Organ regenerative therapy aims to reproduce fully functional organs to replace organs that have been lost or damaged as a result of disease, injury, or aging. For the fully functional regeneration of ectodermal organs, a concept has been proposed in which a bioengineered organ is developed by reproducing the embryonic processes of organogenesis. Here, we show that a bioengineered hair follicle germ, which was reconstituted with embryonic skin-derived epithelial and mesenchymal cells and ectopically transplanted, was able to develop histologically correct hair follicles. The bioengineered hair follicles properly connected to the host skin epithelium by intracutaneous transplantation and reproduced the stem cell niche and hair cycles. The bioengineered hair follicles also autonomously connected with nerves and the arrector pili muscle at the permanent region and exhibited piloerection ability. Our findings indicate that the bioengineered hair follicles could restore physiological hair functions and could be applicable to surgical treatments for alopecia.

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  • In animal models, rodent and human skin with hair follicles can be reconstituted by co-culturing mesenchymal cells with epithelial cells or HF-initiating dermal papilla cells.

    “In animal models, rodent and human skin with HFs can be reconstituted by co-culturing mesenchymal cells with epithelial cells or, specifically, HF initiating dermal papilla (DP) cells (Asakawa et al., 2012; Chuong et al., 2007; Ehama et al., 2007; Ikeda et al., 2009; Nakao et al., 2007; Takagi et al., 2016; Toyoshima et al., 2012; Zheng et al., 2005a).”

    From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Asakawa et al., 2012

    The Methods (Preparation and cultivation of bioengineered hair germ; page not provided) describe combining mouse embryonic epithelial and dermal cells to reconstitute hair follicle germs. The Results (Regeneration of hair follicles by intracutaneous transplantation; page not provided) report regenerated follicles in recipient mouse skin. These passages do not establish reconstitution of human skin with follicles or describe co-culturing epithelial cells specifically with isolated dermal papilla cells. (LLM, conf=0.87)

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Research works printed but not linked: 34

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Authors
Asakawa, K., Toyoshima, K.-E., Ishibashi, N., Tobe, H., Iwadate, A., Kanayama, T., Hasegawa, T., Nakao, K., Toki, H., Noguchi, S., Ogawa, M., Sato, A., & Tsuji, T.
Affiliations
  • Tokyo University of Science (JP)
  • Research Institute for Science and Technology, Tokyo University of Science (JP)
  • Organ Technologies (Japan) (JP)
Corresponding author
Tsuji, Takashi
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2012-05-28
Volume
2
Issue
1
Pages
424-424
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep00424
PMC
PMC3361021
OpenAlex
W1967577266
Cited by
81
References
4
Citations by year
2026: 3 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 11 · 2021: 7 · 2020: 5 · 2019: 4 · 2018: 6 · 2017: 10 · 2016: 8 · 2015: 3 · 2014: 4 · 2013: 5
FWCI
4.42
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science (22249064)
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
Keywords
hair follicle regeneration, organ regeneration, stem cell niche, hair cycle, piloerection, arrector pili muscle, hair loss treatment, nerve innervation
MeSH terms
Alopecia, Animals, Cells, Cultured, Epithelial Cells, Hair, Humans, Mice, Inbred C57BL, Mice, Transgenic, Regeneration, Stem Cells, Skin Transplantation, Microscopy, Confocal, Hair Follicle, Tissue Engineering, Green Fluorescent Proteins, Mice, Bioengineering, Mesenchymal Stem Cells

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