Dendritic spine dynamics leading to spine elimination after repeated inductions of LTD
Open accessAbstract
Memory is fixed solidly by repetition. However, the cellular mechanism underlying this repetition-dependent memory consolidation/reconsolidation remains unclear. In our previous study using stable slice cultures of the rodent hippocampus, we found long-lasting synaptic enhancement/suppression coupled with synapse formation/elimination after repeated inductions of chemical LTP/LTD, respectively. We proposed these phenomena as useful model systems for analyzing repetition-dependent memory consolidation. Recently, we analyzed the dynamics of dendritic spines during development of the enhancement, and found that the spines increased in number following characteristic stochastic processes. The current study investigates spine dynamics during the development of the suppression. We found that the rate of spine retraction increased immediately leaving that of spine generation unaltered. Spine elimination occurred independent of the pre-existing spine density on the dendritic segment. In terms of elimination, mushroom-type spines were not necessarily more stable than stubby-type and thin-type spines.
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Organotypic hippocampal slice cultures undergo key developmental steps similar to in vivo, including synapse maturation.
“Organotypic hippocampal slice cultures are known to undergo key developmental steps similar to those observed in vivo, including synapse maturation28–31, and have been previously used to study ramified microglia function32.”
From Microglia remodel synapses by presynaptic trogocytosis and spine head filopodia induction (2018) · cites this paper as [29]
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A simple method for organotypic cultures of nervous tissue
Stoppini, L., Buchs, P., & Muller, D. (1991) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(91)90128-m · Cited by 3,097 · see citing works
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042 · see citing works
Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex
Trachtenberg, J. T., Chen, B. E., Knott, G. W., Feng, G., Sanes, J. R., Welker, E., & Svoboda, K. (2002) · Nature · doi:10.1038/nature01273 · Cited by 1,927 · see citing works
Stably maintained dendritic spines are associated with lifelong memories
Yang, G., Pan, F., & Gan, W.-B. (2009) · Nature · doi:10.1038/nature08577 · Cited by 1,182 · see citing works
Structure-stability-function relationships of dendritic spines
Kasai, H., Matsuzaki, M., Noguchi, J., Yasumatsu, N., & Nakahara, H. (2003) · Trends in Neurosciences · doi:10.1016/s0166-2236(03)00162-0 · Cited by 847 · see citing works
Experience-dependent and cell-type-specific spine growth in the neocortex
Holtmaat, A., Wilbrecht, L., Knott, G. W., Welker, E., & Svoboda, K. (2006) · Nature · doi:10.1038/nature04783 · Cited by 631 · see citing works
Sorra, K. E., & Harris, K. M. (2000) · Hippocampus · doi:10.1002/1098-1063(2000)10:5 · Cited by 456 · see citing works
Opposite effects of fear conditioning and extinction on dendritic spine remodelling.
Lai, C. S. W., Franke, T. F., & Gan, W.-B. (2012) · Nature · doi:10.1038/nature10792 · Cited by 392 · see citing works
Molecular mechanisms of dendrite stability
Koleske, A. J. (2013) · Nature reviews. Neuroscience · doi:10.1038/nrn3486 · Cited by 351 · see citing works
Principles of Long-Term Dynamics of Dendritic Spines
Yasumatsu, N., Matsuzaki, M., Miyazaki, T., Noguchi, J., & Kasai, H. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.0603-08.2008 · Cited by 314 · see citing works
The Behavior of the Laboratory Rat
(2004) · doi:10.1093/acprof:oso/9780195162851.001.0001 · Cited by 248 · see citing works
Time course of synaptic development in hippocampal organotypic cultures
Müller, D., Buchs, P., & Stoppini, L. (1993) · Developmental Brain Research · doi:10.1016/0165-3806(93)90109-n · Cited by 202 · see citing works
Synapse elimination in the developing cerebellum
Hashimoto, K., & Kano, M. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1405-2 · Cited by 154 · see citing works
Dumitriu, D., Rodriguez, A., & Morrison, J. H. (2011) · Nature Protocols · doi:10.1038/nprot.2011.389 · Cited by 153 · see citing works
Interpyramid Spike Transmission Stabilizes the Sparseness of Recurrent Network Activity
Ikegaya, Y., Sasaki, T., Ishikawa, D., Honma, N., Tao, K., Takahashi, N., Minamisawa, G., Ujita, S., & Matsuki, N. (2012) · Cerebral Cortex · doi:10.1093/cercor/bhs006 · Cited by 144 · see citing works
Long-term depression triggers the selective elimination of weakly integrated synapses
Wiegert, J. S., & Oertner, T. G. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1315926110 · Cited by 139 · see citing works
Elimination of Dendritic Spines with Long-Term Memory Is Specific to Active Circuits
Sanders, J. D., Cowansage, K. K., Baumgärtel, K., & Mayford, M. R. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.1131-12.2012 · Cited by 99 · see citing works
Schacher, S., Kandel, E., & Montarolo, P. (1993) · Neuron · doi:10.1016/0896-6273(93)90056-w · Cited by 73 · see citing works
Campos, V., Prado-Alcalá, R. A., León-Jacinto, U., Aguilar-Vázquez, A. R., Quirarte, G. L., Ramı́rez-Amaya, V., & Díaz-Cintra, S. Y. (2010) · Brain Research · doi:10.1016/j.brainres.2010.10.105 · Cited by 70 · see citing works
Shinoda, Y., Kamikubo, Y., Egashira, Y., Tominaga‐Yoshino, K., & Ogura, A. (2005) · Brain Research · doi:10.1016/j.brainres.2005.02.028 · Cited by 59 · see citing works
Self-citationFeder, H. H., Siegel, H. I., & Wade, G. N. (1974) · Brain Research · doi:10.1016/0006-8993(74)90193-0 · Cited by 56 · see citing works
Sakuragi, S., Tominaga‐Yoshino, K., & Ogura, A. (2013) · Scientific Reports · doi:10.1038/srep03185 · Cited by 55 · see citing works
Self-citationTominaga-Yoshino, K., Urakubo, T., Okada, M., Matsuda, H., & Ogura, A. (2008) · Hippocampus · doi:10.1002/hipo.20391 · Cited by 55 · see citing works
Self-citationTominaga‐Yoshino, K., Kondo, S., Tamotsu, S., & Ogura, A. (2002) · Neuroscience Research · doi:10.1016/s0168-0102(02)00155-4 · Cited by 48 · see citing works
Self-citationPersistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons
Shinoda, Y., Tanaka, T., Tominaga‐Yoshino, K., & Ogura, A. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010390 · Cited by 42 · see citing works
Self-citationKamikubo, Y., Egashira, Y., Tanaka, T., Shinoda, Y., Tominaga‐Yoshino, K., & Ogura, A. (2006) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2006.05032.x · Cited by 41 · see citing works
Self-citationRamiro‐Cortés, Y., & Israely, I. (2013) · PLoS ONE · doi:10.1371/journal.pone.0071155 · Cited by 39 · see citing works
Oe, Y., Tominaga‐Yoshino, K., Hasegawa, S., & Ogura, A. (2013) · Scientific Reports · doi:10.1038/srep01957 · Cited by 37 · see citing works
Self-citationEgashira, Y., Tanaka, T., Soni, P. S., Sakuragi, S., Tominaga‐Yoshino, K., & Ogura, A. (2010) · Journal of Neuroscience Research · doi:10.1002/jnr.22505 · Cited by 30 · see citing works
Self-citationKawaai, K., Tominaga‐Yoshino, K., Urakubo, T., et al. (2010) · Journal of Neuroscience Research · doi:10.1002/jnr.22457 · Cited by 24 · see citing works
Self-citationShort protocols in neuroscience : systems and behavioral methods
Crawley, J. N. (2007) · Wiley eBooks · Cited by 17 · see citing works
Intracellular injection in fixed slices: obtaining complete dendritic arbors of large cells
Pace, C. J., Tieman, D. G., & Tieman, S. B. (2002) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(02)00152-8 · Cited by 17 · see citing works
Urakubo, T., Ogura, A., & Tominaga‐Yoshino, K. (2006) · Neuroscience Letters · doi:10.1016/j.neulet.2006.07.057 · Cited by 13 · see citing works
Self-citationOe, Y., Tominaga‐Yoshino, K., & Ogura, A. (2011) · Journal of Neuroscience Research · doi:10.1002/jnr.22668 · Cited by 5 · see citing works
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- Authors
- Hasegawa, S., Sakuragi, S., Tominaga-Yoshino, K., & Ogura, A.
- Affiliations
- The University of Osaka (JP)
- Corresponding author
- Ogura, Akihiko
- ORCID
- Sakuragi, Shigeo
- Venue
- Scientific Reports
- Published
- 2015-01-09
- Volume
- 5
- Issue
- 1
- Pages
- 7707-7707
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4648349
- OpenAlex
- W2041421565
- Cited by
- 55
- References
- 34
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 6 · 2021: 1 · 2020: 8 · 2019: 7 · 2018: 5 · 2017: 9 · 2016: 6 · 2015: 1
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Scientific Reports (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (24650207)
- Ministry of Education, Science and Technology
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- dendritic spine dynamics, long-term depression, synaptic plasticity, memory consolidation, hippocampus, slice culture, mushroom spines
- MeSH terms
- Animals, Fluorescent Dyes, Hippocampus, Methoxyhydroxyphenylglycol, Mice, Transgenic, Synapses, Time Factors, Long-Term Potentiation, Microscopy, Confocal, Dendritic Spines, Mice, In Vitro Techniques
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