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Cited by 295

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes

Open access
Nakase, Ikuhiko, Futaki, Shiroh·2015·Scientific Reports
DOI 10.1038/srep10112
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Abstract

Exosomes, which are approximately 100 nm vesicles secreted by cells, have been studied with respect to cell-to-cell communication, disease diagnosis and intracellular delivery. The cellular uptake of exosomes occurs by endocytosis; however, the cytosolic release efficiency of encapsulated molecules inside cells is low. To address this issue, here we demonstrate a simple technique for enhancing the cellular uptake and cytosolic release of exosomes by combining a pH-sensitive fusogenic peptide for the fusion of endosomal and exosomal membranes inside cells. This method stimulates the efficient cytosolic release of the exosomal contents with cationic lipids that act as a “glue” to support cellular uptake. Using this simple combined technique, the effective cellular uptake and cytosolic release of an artificially encapsulated dextran macromolecule (70 kDa) in exosomes are achieved and a marked improvement in bioactivity is attained with the artificially encapsulated ribosome-inactivating protein saporin. Our method will contribute to many biological research fields, including the assessment of the activities of exosomal contents and the development of candidate tools enabling intracellular visualisation and cellular regulation for future therapeutic applications.

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Metadata

Authors
Nakase, I., & Futaki, S.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2015-05-26
Volume
5
Issue
1
Pages
10112-10112
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep10112
OpenAlex
W2256381580
Cited by
295
References
38
FWCI
5.15
Type
journal article
Language
en
Open access
gold

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