Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates
Open accessAbstract
A main function of splenic red pulp macrophages is the degradation of damaged or aged erythrocytes. Here we show that these macrophages accumulate ferrimagnetic iron oxides that render them intrinsically superparamagnetic. Consequently, these cells routinely contaminate splenic cell isolates obtained with the use of MCS, a technique that has been widely used in immunological research for decades. These contaminations can profoundly alter experimental results. In mice deficient for the transcription factor SpiC, which lack red pulp macrophages, liver Kupffer cells take over the task of erythrocyte degradation and become superparamagnetic. We describe a simple additional magnetic separation step that avoids this problem and substantially improves purity of magnetic cell isolates from the spleen.
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RPM and KC express the complete machinery for RBC clearance and heme iron recycling.
“It is well established that RPM and KC express the complete machinery for RBC clearance and heme iron recycling [72, 88, 231].”
From “Pumping iron”—how macrophages handle iron at the systemic, microenvironmental, and cellular levels (2017) · cites this paper as [72]
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- Authors
- Franken, L., Klein, M., Spasova, M., Elsukova, A., Wiedwald, U., Welz, M., Knolle, P., Farle, M., Limmer, A., & Kurts, C.
- Affiliations
- University of Bonn (DE)
- University of Duisburg-Essen (DE)
- Technical University of Munich (DE)
- University Hospital Bonn (DE)
- Corresponding author
- Franken, Lars
- Kurts, Christian
- ORCID
- Venue
- Scientific Reports
- Published
- 2015-08-11
- Volume
- 5
- Issue
- 1
- Pages
- 12940-12940
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4531335
- OpenAlex
- W2285674543
- Cited by
- 54
- References
- 24
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 6 · 2019: 8 · 2018: 4 · 2017: 11 · 2016: 5
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFBTR57, SFB 704, SFB 645)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- superparamagnetism, Kupffer cells, spleen
- MeSH terms
- Animals, Blood Cell Count, Cell Separation, Erythrocytes, Flow Cytometry, Humans, Liver, Macrophages, Phagocytosis, Spleen, Cell Lineage, Mice, Ferrosoferric Oxide
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