Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China
Open accessAbstract
Microbial secondary metabolites are valuable resources for novel drug discovery. In particular, actinomycetes expressed a range of antibiotics against a spectrum of bacteria. In genus level, strain Allosalinactinospora lopnorensis CA15-2 T is the first new actinomycete isolated from the Lop Nor region, China. Antimicrobial assays revealed that the strain could inhibit the growth of certain types of bacteria, including Acinetobacter baumannii and Staphylococcus aureus, highlighting its clinical significance. Here we report the 5,894,259 base pairs genome of the strain, containing 5,662 predicted genes, and 832 of them cannot be detected by sequence similarity-based methods, suggesting the new species may carry a novel gene pool. Furthermore, our genome-mining investigation reveals that A. lopnorensis CA15-2 T contains 17 gene clusters coding for known or novel secondary metabolites. Meanwhile, at least six secondary metabolites were disclosed from ethyl acetate (EA) extract of the fermentation broth of the strain by high-resolution UPLC-MS. Compared with reported clusters of other species, many new genes were found in clusters, and the physical chromosomal location and order of genes in the clusters are distinct. This study presents evidence in support of A. lopnorensis CA15-2 T as a potent natural products source for drug discovery.
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More than 22,000 known microbial secondary metabolites exist, 70% of which are produced by actinomycetes, 20% by fungi, 7% by Bacillus spp. and 1-2% by other bacteria.
“There are more than 22,000 known microbial secondary metabolites, 70% of which are produced by actinomycetes, 20% by fungi, 7% by Bacillus spp. and 1–2% by other bacteria [57].”
From Marine Natural Products: A Source of Novel Anticancer Drugs (2019) · cites this paper as [57]
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Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669 · see citing works
MUSCLE: multiple sequence alignment with high accuracy and high throughput
Edgar, R. C. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh340 · Cited by 44,662 · see citing works
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011) · Molecular Biology and Evolution · doi:10.1093/molbev/msr121 · Cited by 39,795 · see citing works
Manual of Clinical Microbiology.
(1980) · Annals of Internal Medicine · doi:10.7326/0003-4819-93-6-939_2 · Cited by 14,435 · see citing works
Prodigal: prokaryotic gene recognition and translation initiation site identification
Hyatt, D., Chen, G.-L., LoCascio, P. F., Land, M., Larimer, F. W., & Hauser, L. (2010) · BMC Bioinformatics · doi:10.1186/1471-2105-11-119 · Cited by 12,426 · see citing works
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
Lowe, T. M., & Eddy, S. R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.5.955 · Cited by 11,120 · see citing works
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
Lagesen, K., Hallin, P., Rødland, E., Stærfeldt, H., Rognes, T., & Ussery, D. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm160 · Cited by 5,965 · see citing works
OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes
Li, L., Stoeckert, C. J., & Roos, D. S. (2003) · Genome Research · doi:10.1101/gr.1224503 · Cited by 5,707 · see citing works
Bioactive Microbial Metabolites: A Personal View
Bérdy, J. (2005) · The Journal of Antibiotics · doi:10.1038/ja.2005.1 · Cited by 3,170 · see citing works
Multiple Sequence Alignment Using ClustalW and ClustalX
Thompson, J., Gibson, T. J., & Higgins, D. G. (2002) · Current Protocols in Bioinformatics · doi:10.1002/0471250953.bi0203s00 · Cited by 2,989 · see citing works
Improved microbial gene identification with GLIMMER
Delcher, A. L. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.23.4636 · Cited by 2,493 · see citing works
Arnison, P. G., Bibb, M. J., Bierbaum, G., Bowers, A. A., Bugni, T. S., Bulaj, G. W., Camarero, J. A., Campopiano, D. J., Challis, G. L., Clardy, J., et al. (2012) · Natural Product Reports · doi:10.1039/c2np20085f · Cited by 2,065 · see citing works
An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii
Dijkshoorn, L., Nemec, A., & Seifert, H. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1789 · Cited by 1,832 · see citing works
Microbial life at high salt concentrations: phylogenetic and metabolic diversity
Oren, A. (2008) · Saline Systems · doi:10.1186/1746-1448-4-2 · Cited by 994 · see citing works
Maragakis, L. L., & Perl, T. M. (2008) · Clinical Infectious Diseases · doi:10.1086/529198 · Cited by 972 · see citing works
Diversity of halophilic microorganisms: Environments, phylogeny, physiology, and applications
Oren, A. (2002) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj/jim/7000176 · Cited by 833 · see citing works
antiSMASH 2.0—a versatile platform for genome mining of secondary metabolite producers
Blin, K., Medema, M. H., Kazempour, D., Fischbach, M. A., Rainer Breitling, A., Eriko Takano, A., & Weber, T. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt449 · Cited by 798 · see citing works
Brown, A. D. (1976) · Bacteriological Reviews · doi:10.1128/br.40.4.803-846.1976 · Cited by 754 · see citing works
Schroeckh, V., Scherlach, K., Nützmann, H., Shelest, E., Schmidt‐Heck, W., Schuemann, J., Martin, K., Hertweck, C., & Brakhage, A. A. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0901870106 · Cited by 662 · see citing works
Loper, J. E., Hassan, K. A., Mavrodi, D. V., Davis, E. W., Lim, C. K., Shaffer, B. T., Elbourne, L. D. H., Stockwell, V. O., Hartney, S. L., Breakwell, K., et al. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002784 · Cited by 645 · see citing works
eggNOG v3.0: orthologous groups covering 1133 organisms at 41 different taxonomic ranges
Powell, S. T., Szklarczyk, D., Trachana, K., Roth, A. C., Kuhn, M., Muller, J., Arnold, R. R., Rattei, T., Letunić, I., Doerks, T., et al. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr1060 · Cited by 564 · see citing works
Udwary, D., Allen, L. Z., Asolkar, R. N., Singan, V. R., Lapidus, A. L., Fenical, W., Jensen, P. R., & Moore, B. S. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700962104 · Cited by 537 · see citing works
van Heel, A. J., de Jong, A., Montalbán‐López, M., Kok, J., & Kuipers, O. P. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt391 · Cited by 477 · see citing works
Yamanaka, K., Reynolds, K. A., Kersten, R. D., Ryan, K. S., Gonzalez, D. J., Nizet, V., Dorrestein, P. C., & Moore, B. S. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1319584111 · Cited by 475 · see citing works
Marine actinomycetes: An ongoing source of novel bioactive metabolites
Subramani, R., & Aalbersberg, W. G. L. (2012) · Microbiological Research · doi:10.1016/j.micres.2012.06.005 · Cited by 456 · see citing works
Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans
Stinear, T. P., Mve-Obiang, A., Small, P. L. C., Frigui, W., Pryor, M. J., Brosch, R., Jenkin, G., Johnson, P. D. R., Davies, J. K., Lee, R. E., Jr., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0305877101 · Cited by 390 · see citing works
Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China
Hong, K., Gao, A.-H., Xie, Q.-Y., Gao, H. G., Zhuang, L., Lin, H.-P., Yu, H.-P., Li, J., Yao, X.-S., Goodfellow, M., et al. (2009) · Marine Drugs · doi:10.3390/md7010024 · Cited by 372 · see citing works
Discovery of a new peptide natural product by Streptomyces coelicolor genome mining
Lautru, S., Deeth, R. J., Bailey, L. M., & Challis, G. L. (2005) · Nature Chemical Biology · doi:10.1038/nchembio731 · Cited by 363 · see citing works
Schwibbert, K., Marı́n-Sanguino, A., Bagyan, I., Heidrich, G., Lentzen, G., Seitz, H., Rampp, M., Schuster, S. C., Klenk, H., Pfeiffer, F., et al. (2010) · Environmental Microbiology · doi:10.1111/j.1462-2920.2010.02336.x · Cited by 281 · see citing works
McAlpine, J. B., Bachmann, B. O., Piraee, M., Tremblay, S., Alarco, A., Zazopoulos, E., & Farnet, C. M. (2005) · Journal of Natural Products · doi:10.1021/np0401664 · Cited by 231 · see citing works
Marine actinomycetes as an emerging resource for the drug development pipelines
Zotchev, S. B. (2011) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2011.06.002 · Cited by 176 · see citing works
Magarvey, N. A., Beck, Z. Q., Golakoti, T., Ding, Y., Huber, U., Hemscheidt, T. K., Abelson, D. M., Moore, R. E., & Sherman, D. H. (2006) · ACS Chemical Biology · doi:10.1021/cb6004307 · Cited by 167 · see citing works
Pyranonaphthoquinone antibiotics—isolation, structure and biological activity
Brimble, M. A., Nairn, M. R., & Duncalf, L. J. (1999) · Natural Product Reports · doi:10.1039/a804287j · Cited by 164 · see citing works
Gross, H. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-007-0900-5 · Cited by 151 · see citing works
Coutanceau, E., Marsollier, L., Brosch, R., Perret, E., Goossens, P. L., Tanguy, M., Cole, S. T., Small, P. L. C., & Demangel, C. (2005) · Cellular Microbiology · doi:10.1111/j.1462-5822.2005.00546.x · Cited by 150 · see citing works
Precursor-centric genome-mining approach for lasso peptide discovery
Maksimov, M. O., Pelczer, I., & Link, A. J. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208978109 · Cited by 147 · see citing works
Apoptolidin, a selective cytotoxic agent, is an inhibitor of F0F1-ATPase
Salomon, A. R., Voehringer, D. W., Herzenberg, L. A., & Khosla, C. (2001) · Chemistry & Biology · doi:10.1016/s1074-5521(00)00057-0 · Cited by 141 · see citing works
Beginner’s guide to comparative bacterial genome analysis using next-generation sequence data
Edwards, D., & Holt, K. E. (2013) · Microbial Informatics and Experimentation · doi:10.1186/2042-5783-3-2 · Cited by 139 · see citing works
Sequence-Based Analysis of Secondary-Metabolite Biosynthesis in Marine Actinobacteria
Gontang, E. A., Gaudêncio, S. P., Fenical, W., & Jensen, P. R. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.02852-09 · Cited by 127 · see citing works
Letzel, A., Pidot, S. J., & Hertweck, C. (2014) · BMC Genomics · doi:10.1186/1471-2164-15-983 · Cited by 121 · see citing works
Biosynthetic Gene Cluster of Simocyclinone, a Natural Multihybrid Antibiotic
Trefzer, A. C., Pelzer, S., Schimana, J., Stockert, S., Bihlmaier, C., Fiedler, H., Welzel, K., Vente, A., & Bechthold, A. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.5.1174-1182.2002 · Cited by 116 · see citing works
Simocyclinone D8, an Inhibitor of DNA Gyrase with a Novel Mode of Action
Flatman, R. H., Howells, A. J., Heide, L. E., Fiedler, H., & Maxwell, A. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.3.1093-1100.2005 · Cited by 115 · see citing works
DoBISCUIT: a database of secondary metabolite biosynthetic gene clusters
Ichikawa, N., Sasagawa, M., Yamamoto, M., Komaki, H., Yoshida, Y., Yamazaki, S., & Fujita, N. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1177 · Cited by 115 · see citing works
Cánovas, D., Vargas, C., Calderón, M. I., Ventosa, A., & Nieto, J. J. (1998) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(98)80060-x · Cited by 104 · see citing works
Berberine as a Promising Safe Anti-Cancer Agent- Is there a Role for Mitochondria?
Diogo, C. V., Machado, N. G., Barbosa, I. A., Serafim, T. L., Burgeiro, A., & Oliveira, P. J. (2011) · Current Drug Targets · doi:10.2174/138945011795528930 · Cited by 104 · see citing works
Guo, L., Tuo, L., Habden, X., Zhang, Y., Liu, J., Jiang, Z.-K., Liu, S., Tohty, D., & Sun, C. (2014) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.055160-0 · Cited by 98 · see citing works
Self-citationZhao, C., Coughlin, J. M., Ju, J., Zhu, D., Wendt-Pienkowski, E., Zhou, X., Wang, Z., Shen, B., & Deng, Z. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.090092 · Cited by 87 · see citing works
Treatment of Acinetobacter infections
Bergogne-Bérézin, E. (1997) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.6.2.119 · Cited by 81 · see citing works
Ballav, S., Kerkar, S., Thomas, S., & Augustine, N. (2014) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.08.017 · Cited by 78 · see citing works
Prospecting genomes for lasso peptides
Maksimov, M. O., & Link, A. J. (2013) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-013-1357-4 · Cited by 77 · see citing works
Hu, Y., Chou, W. K. W., Hopson, R., & Cane, D. E. (2011) · Chemistry & Biology · doi:10.1016/j.chembiol.2010.11.008 · Cited by 76 · see citing works
Subramani, R., Kumar, R., Prasad, P., & Aalbersberg, W. G. L. (2013) · Asian Pacific Journal of Tropical Biomedicine · doi:10.1016/s2221-1691(13)60065-9 · Cited by 69 · see citing works
How Many “Me-Too” Drugs Is Too Many?
Gagne, J. J. (2011) · JAMA · doi:10.1001/jama.2011.152 · Cited by 68 · see citing works
Göller, K., Ofer, A., & Galinski, E. A. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb12960.x · Cited by 65 · see citing works
New diketopiperazine derivatives from a deep-sea-derived Nocardiopsis alba SCSIO 03039
Zhang, Q., Li, S., Chen, Y., Tian, X., Zhang, H., Zhang, G., Zhu, Y., Zhang, S., Zhang, W., & Zhang, C. (2012) · The Journal of Antibiotics · doi:10.1038/ja.2012.88 · Cited by 52 · see citing works
Ding, Z., Zhao, J., Li, M., Huang, R., Li, Q., Cui, X., Zhu, H., & Wen, M. (2012) · Journal of Natural Products · doi:10.1021/np3004936 · Cited by 51 · see citing works
Carter, G. T., Nietsche, J. A., WILLIAMS, D. R., & Borders, D. B. (1990) · The Journal of Antibiotics · doi:10.7164/antibiotics.43.504 · Cited by 49 · see citing works
Göller, K. (1998) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(98)00086-x · Cited by 45 · see citing works
Imai, S., Shimazu, A., FURIHATA, K., Furihata, K., HAYAKAWA, Y., & Seto, H. (1990) · The Journal of Antibiotics · doi:10.7164/antibiotics.43.1606 · Cited by 40 · see citing works
Yassin, A. S., & Mankin, A. S. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m703106200 · Cited by 39 · see citing works
Simocyclinones: diversity of metabolites is dependent on fermentation conditions 3
Schimana, J., Walker, M., Zeeck, A., & Fiedler, H.-P. (2001) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj.jim.7000024 · Cited by 33 · see citing works
Isolation, Structure Determination, and Anti-Cancer Activity of Apoptolidin D
Wender, P. A., & Longcore, K. E. (2007) · Organic Letters · doi:10.1021/ol0630245 · Cited by 31 · see citing works
Morgan, D. (1995) · Trends in Microbiology · doi:10.1016/s0966-842x(00)89003-4 · Cited by 29 · see citing works
Actinosynnema mirum, a new producer of nocardicin antibiotics.
Watanabe, K., Okuda, T., Yokose, K., FURUMAI, T., & MARUYAMA, H. B. (1983) · The Journal of Antibiotics · doi:10.7164/antibiotics.36.321 · Cited by 26 · see citing works
New Pyran‐2‐ones from Alkalophilic Actinomycete, Nocardiopsis alkaliphila sp. Nov. YIM‐80379
Wang, Z., Fu, P., Liu, P., Wang, P., Hou, J., Li, W., & Zhu, W. (2013) · Chemistry & Biodiversity · doi:10.1002/cbdv.201200086 · Cited by 22 · see citing works
A new phenoxazine derivative isolated from marine sediment actinomycetes, Nocardiopsis sp. 236
Lu, C., Li, Y., Wang, H., Wang, B. M., & Shen, Y. (2013) · Drug Discoveries & Therapeutics · doi:10.5582/ddt.2013.v7.3.101 · Cited by 21 · see citing works
Beck, Z. Q., Burr, D. A., & Sherman, D. H. (2007) · ChemBioChem · doi:10.1002/cbic.200700162 · Cited by 18 · see citing works
Computational Methods for Gene Finding in Prokaryotes
Angelova, M., Kalajdziski, S., & Kocarev, L. (2010) · Cited by 17 · see citing works
Soror, S. H. A., Rao, R., & Cullum, J. (2009) · Protein Engineering Design and Selection · doi:10.1093/protein/gzp009 · Cited by 15 · see citing works
[Actinomycetes from mangrove and their secondary metabolites].
Hong, K. (2013) · PubMed · Cited by 14 · see citing works
The unusual macrocycle forming thioesterase of mycolactone
Meier, J. L., Barrows-Yano, T., Foley, T. L., Wike, C. L., & Burkart, M. D. (2008) · Molecular BioSystems · doi:10.1039/b801397g · Cited by 12 · see citing works
[Biodiversity and functional enzymes of cultured halophilic archaeon in Lop Nur region].
Liu, B., Tang, S., Hong, M., He, S., Nie, G., Guan, T., Zhang, L., & Li, W. (2011) · PubMed · Cited by 2 · see citing works
Biosynthetic Study of Actinomycetes-Metabolites for Creating Novel Analogs
Ito, T. (2013) · YAKUGAKU ZASSHI · doi:10.1248/yakushi.13-00175 · Cited by 1 · see citing works
[Endophyte microbial community in Achnatherum inebrians].
Shi, Y., Zhang, X., & Lou, K. (2012) · PubMed · Cited by 1 · see citing works
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1 of the 75 references couldn’t be matched to a known paper.
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Khalifa, S. A. M., Elias, N., Farag, M. A., Chen, L., Saeed, A., Hegazy, M.-E. F., Moustafa, M. S., Abd El-Wahed, A., Al-Mousawi, S. M., Musharraf, S. G., et al. (2019) · Marine Drugs · doi:10.3390/md17090491 · Cited by 555 · see citing works
Sources of antibiotics: Hot springs
Mahajan, G., & Balachandran, L. (2016) · Biochemical Pharmacology · doi:10.1016/j.bcp.2016.11.021 · Cited by 91 · see citing works
Jose, P. A., & Jha, B. (2017) · Scientific Reports · doi:10.1038/s41598-017-09672-6 · Cited by 75 · see citing works
Masand, M., Sivakala, K. K., Menghani, E., Thinesh, T., Anandham, R., Sharma, G., Sivakumar, N., Jebakumar, S. R. D., & Jose, P. A. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00687 · Cited by 42 · see citing works
Qi, D., Zou, L., Zhou, D., Zhang, M., Wei, Y., Li, K., Zhao, Y., Zhang, L., & Xie, J. (2022) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-022-11788-3 · Cited by 42 · see citing works
Hu, D., Sun, C., Jin, T., Fan, G., Mok, K. M., Li, K., & Lee, S. M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01540 · Cited by 29 · see citing works
Self-citationAnti-inflammatory, anti-infectious and anti-cancer potential of marine algae and sponge: A review
Naqvi, S. A. R., Sherazi, T. A., Hassan, S. U., Shahzad, S. A., & Faheem, Z. (2022) · European Journal of Inflammation · doi:10.1177/20587392221075514 · Cited by 14 · see citing works
Hu, D., Lee, S. M., Li, K., & Mok, K. M. (2022) · Frontiers in Marine Science · doi:10.3389/fmars.2022.700685 · Cited by 7 · see citing works
Self-citationNon-Pharmaceutical Interventions in Cancer Therapy
Tyagi, V., Tanwar, N., Kumar, N., Phogat, N., Kumar, P., & Kumar, V. (2024) · Current Cancer Therapy Reviews · doi:10.2174/0115733947268161240404084729 · Cited by 2 · see citing works
Anti-Microbial Drugs for Emerging and Re-emerging Microbial Diseases: Paradigm in the 21st Century
Swaroop, S., Verma, N., Yadav, J. K., & Pandey, J. (2023) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815080056123020004
Lu, J., Yuan, J., Xue, S., Zhang, R., Zhu, Y., Gong, S., & Zheng, B. (2026) · Molecular Genetics and Genomics · doi:10.1007/s00438-026-02443-x
- Authors
- Huang, C., Leung, R. K.-K., Guo, M., Tuo, L., Guo, L., Yew, W. W., Lou, I., Lee, S. M. Y., & Sun, C.
- Affiliations
- University of Macau (MO)
- Chinese University of Hong Kong (HK)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Lee, Simon Ming Yuen
- Venue
- Scientific Reports
- Published
- 2016-02-11
- Volume
- 6
- Issue
- 1
- Pages
- 20667-20667
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4749953
- OpenAlex
- W2292000244
- Cited by
- 11
- References
- 75
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81321004, MYRG2015-00214-ICMS-QRCM, 81373308, MYRG139(Y1-L4)-ICMS12-LMY, 81328025)
- Science and Technology Development Fund
- Universidade de Macau (MYRG2015-00214- ICMS-QRCM, MYRG139(Y1-L4)-ICMS12-LMY)
- Fundo para o Desenvolvimento das Ciências e da Tecnologia
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- actinomycetes, genome mining, gene clusters, new natural products, antimicrobial activity, Acinetobacter baumannii, Staphylococcus aureus, new species, drug discovery
- MeSH terms
- Acetates, Antibiosis, Anti-Bacterial Agents, Biological Products, China, Chromosome Mapping, Multigene Family, Phylogeny, Solvents, Staphylococcus aureus, Genome, Bacterial, Sequence Analysis, DNA, Actinobacteria, Acinetobacter baumannii, Disk Diffusion Antimicrobial Tests, Drug Discovery, Genome Size, Secondary Metabolism
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