The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
A novel method for preparing microplastic fibers
Open accessAbstract
Microscopic plastic (microplastic, 0.1 µm–5 mm) is a widespread pollutant impacting upon aquatic ecosystems across the globe. Environmental sampling has revealed synthetic fibers are prevalent in seawater, sediments and biota. However, microplastic fibers are rarely used in laboratory studies as they are unavailable for purchase and existing preparation techniques have limited application. To facilitate the incorporation of environmentally relevant microplastic fibers into future studies, new methods are required. Here, a novel cryotome protocol has been developed. Nylon, polyethylene terephthalate and polypropylene fibers (10–28 μm diameter) were aligned, embedded in water-soluble freezing agent and sectioned (40–100 μm length) using a cryogenic microtome. Microplastic fibers were prepared to specified lengths (P < 0.05, ANOVA) and proved consistent in size. Fluorescent labelling of Nylon microfibers with Nile Red facilitated imaging. A 24 h feeding experiment confirmed bioavailability of 10 × 40 μm Nylon fibers to brine shrimp (Artemia sp). This protocol provides a consistent method for preparing standardised fibrous microplastics, with widths similar to those observed in the natural environment, which could ultimately lead to a better understanding of the biological and ecological effects of microplastic debris in the environment.
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Plastic debris in the open ocean
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The impact of debris on marine life
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The deep sea is a major sink for microplastic debris
Woodall, L. C., Sanchez-Vidal, A., Canals, M., Paterson, G. L. J., Coppock, R., Sleight, V., Calafat, A., Rogers, A. D., Narayanaswamy, B. E., & Thompson, R. C. (2014) · Royal Society Open Science · doi:10.1098/rsos.140317 · Cited by 1,946
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Self-citationMechanisms in the Pathogenesis of Asbestosis and Silicosis
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Cited by
Microplastics in Freshwaters and Drinking Water: Critical Review and Assessment of Data Quality
Koelmans, A. A., Mohamed Nor, N. H., Hermsen, E., Kooi, M., Mintenig, S. M., & De France, J. (2019) · Water Research · doi:10.1016/j.watres.2019.02.054 · Cited by 2,535
Erni-Cassola, G., Gibson, M. I., Thompson, R. C., & Christie-Oleza, J. A. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b04512 · Cited by 848
Simplifying Microplastic via Continuous Probability Distributions for Size, Shape, and Density
Kooi, M., & Koelmans, A. A. (2019) · Environmental Science & Technology Letters · doi:10.1021/acs.estlett.9b00379 · Cited by 639
Gago, J., Carretero, O., Filgueiras, A. V., & Viñas, L. (2018) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2017.11.070 · Cited by 503
Role of Marine Snows in Microplastic Fate and Bioavailability
Porter, A., Lyons, B. P., Galloway, T. S., & Lewis, C. (2018) · Environmental Science & Technology · doi:10.1021/acs.est.8b01000 · Cited by 419
Metadata
- Authors
- Cole, M.
- Venue
- Scientific Reports
- Published
- 2016-10-03
- Volume
- 6
- Issue
- 1
- Pages
- 34519-34519
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2528566096
- Cited by
- 332
- References
- 10
- FWCI
- 7.56
- Type
- journal article
- Language
- en
- Open access
- gold
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