The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer
Open accessAbstract
Extracellular vesicles (EVs), including exosomes and microvesicles, are present in a variety of bodily fluids, and the concentration of these sub-cellular vesicles and their associated biomarkers (proteins, nucleic acids, and lipids) can be used to aid clinical diagnosis. Although ultracentrifugation is commonly used for isolation of EVs, it is highly time-consuming, labor-intensive and instrument-dependent for both research laboratories and clinical settings. Here, we developed an integrated double-filtration microfluidic device that isolated and enriched EVs with a size range of 30–200 nm from urine, and subsequently quantified the EVs via a microchip ELISA. Our results showed that the concentration of urinary EVs was significantly elevated in bladder cancer patients (n = 16) compared to healthy controls (n = 8). Receiver operating characteristic (ROC) analysis demonstrated that this integrated EV double-filtration device had a sensitivity of 81.3% at a specificity of 90% (16 bladder cancer patients and 8 healthy controls). Thus, this integrated device has great potential to be used in conjunction with urine cytology and cystoscopy to improve clinical diagnosis of bladder cancer in clinics and at point-of-care (POC) settings.
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Cites
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EAU Guidelines on Non–Muscle-invasive Urothelial Carcinoma of the Bladder: Update 2013
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Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen
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Cited by
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Exosomes as a new frontier of cancer liquid biopsy
Yu, D., Li, Y., Wang, M., Gu, J., Xu, W., Cai, H., Fang, X., & Zhang, X. (2022) · Molecular Cancer · doi:10.1186/s12943-022-01509-9 · Cited by 855
Exosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery
Antimisiaris, S. G., Mourtas, S., & Marazioti, A. (2018) · doi:10.20944/preprints201810.0507.v1 · Cited by 605
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
Ramirez-Garrastacho, M., Bajo-Santos, C., Line, A., Martens-Uzunova, E. S., de la Fuente, J. M., Moros, M., Soekmadji, C., Tasken, K. A., & Llorente, A. (2022) · British Journal of Cancer · doi:10.1038/s41416-021-01610-8 · Cited by 130
Metadata
- Authors
- Liang, L.-G., Kong, M.-Q., Zhou, S., Sheng, Y.-F., Wang, P., Yu, T., Inci, F., Kuo, W. P., Li, L.-J., Demirci, U., & Wang, S.
- Venue
- Scientific Reports
- Published
- 2017-04-24
- Volume
- 7
- Issue
- 1
- Pages
- 46224-46224
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2607716704
- Cited by
- 282
- References
- 29
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- gold
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- Liang, L.-G., Kong, M.-Q., Zhou, S., Sheng, Y.-F., Wang, P., Yu, T., Inci, F., Kuo, W. P., Li, L.-J., Demirci, U., & Wang, S. (2017). An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer. Scientific Reports. https://doi.org/10.1038/srep46224
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