The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies
Abstract
Here we demonstrate a microfluidic perfusion system suitable for a long-term (>2 week) culture of muscle cells spanning the whole process of differentiation from myoblasts to myotubes. Cell-adhesive surface microdomains alternating with a robust cell-repellent coating mimic in vivo spatial cues for muscle cell assembly and allow for confining the fusion of myoblasts into aligned, isolated multinucleated myotubes. The microfluidic system provides accurate control of the perfusion rates and biochemical composition of the environment surrounding the cells. Comparing muscle cell-specific differentiation markers and the timing of fusion, we observed no differences in differentiation between microfluidic and traditional cultures. All differentiation assays were fully microfluidic, i.e. they were performed by sequentially changing the fluids in the micro-channels. By delivering fluorescent markers using heterogeneous laminar flows, it was possible to confine a membrane receptor labeling assay to a region smaller than a myotube. Our method can serve as an improved in vitro model for studying muscle cell differentiation and for characterizing extracellular molecules and mechanisms involved in neuromuscular differentiation.
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Chaotic Mixer for Microchannels
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Biotechnology at low Reynolds numbers
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Integration of Cell Culture and Microfabrication Technology
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Passively Driven Integrated Microfluidic System for Separation of Motile Sperm
Cho, B. S., Schuster, T. G., Zhu, X., Chang, D., Smith, G. D., & Takayama, S. (2003) · Analytical Chemistry · doi:10.1021/ac020579e · Cited by 356
Development of a Microscale Cell Culture Analog To Probe Naphthalene Toxicity
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Self-citationMicrofluidic technology for assisted reproduction
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Cited by
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Bhattacharjee, N., Urrios, A., Kang, S., & Folch, A. (2016) · Lab on a Chip · doi:10.1039/c6lc00163g · Cited by 1,123
Self-citationEngineered Microenvironments for Controlled Stem Cell Differentiation
Burdick, J. A., & Vunjak-Novakovic, G. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0131 · Cited by 485
Microfluidics technology for manipulation and analysis of biological cells
Yi, C., Li, C.-W., Ji, S., & Yang, M. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2005.12.037 · Cited by 478
A Review of Three-Dimensional In Vitro Tissue Models for Drug Discovery and Transport Studies
Elliott, N. T., & Yuan, F. (2011) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.22257 · Cited by 449
A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Toh, Y.-C., Zhang, C., Zhang, J., Khong, Y. M., Chang, S., Samper, V. D., van Noort, D., Hutmacher, D. W., & Yu, H. (2007) · Lab on a Chip · doi:10.1039/b614872g · Cited by 414
Metadata
- Authors
- Tourovskaia, A., Figueroa-Masot, X., & Folch, A.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2005
- Volume
- 5
- Issue
- 1
- Pages
- 14
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2152103726
- Cited by
- 363
- References
- 13
- FWCI
- 18.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tourovskaia, A., Figueroa-Masot, X., & Folch, A. (2005). Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies. Lab on a Chip. https://doi.org/10.1039/b405719h
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
