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Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap
Abstract
Biomimetic heterogeneous patterning of hepatic and endothelial cells, which start from randomly distributed cells inside the microfluidic chamber, via the chip design of enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported in this paper. The concentric-stellate-tip electrode array design in this chip generates radial-pattern electric fields for the DEP manipulation of the live liver cells. By constructing the geometric shape and the distribution of stellate tips, the DEP electrodes enhance the desired spatial electric-field gradients to guide and snare individual cells to form the desired biomimetic pattern. With this proposed microfluidic chip design, the original randomly distributed hepatocytes inside the microfluidic chamber can be manipulated in parallel and align into the desired pearl-chain array pattern. This radial pattern mimics the lobular morphology of real liver tissue. The endothelial cells, then, are snared into the additional pearl-chain array and settle at the space in-between the previous hepatic pearl-chain array. By this cell-lab chip, we demonstrate the in vitro reconstruction of the heterogeneous lobule-mimetic radial pattern with good cell viability after cell patterning. This work reports the rapid in-parallel patterning of the dual types of live liver cells via the enhanced DEP trap inside the microfluidic chip.
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Cited by
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280
Lee, H., & Cho, D.-W. (2016) · Lab on a Chip · doi:10.1039/c6lc00450d · Cited by 363
Gagnon, Z. R. (2011) · ELECTROPHORESIS · doi:10.1002/elps.201100060 · Cited by 317
Organ-on-a-chip platforms: a convergence of advanced materials, cells, and microscale technologies
Ahadian, S., Civitarese, R., Bannerman, D., Mohammadi, M. H., Lu, R., Wang, E., Davenport‐Huyer, L., Lai, B., Zhang, B., Zhao, Y., et al. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201700506 · Cited by 315
Organs-on-chips: breaking the in vitro impasse
Meer, A. D. V. D., & Berg, A. V. D. (2012) · Integrative Biology · doi:10.1039/c2ib00176d · Cited by 314
Rare cell isolation and analysis in microfluidics
Chen, Y., Li, P., Huang, P.-H., Xie, Y., Mai, J. D., Wang, L., Nguyen, N.-T., & Huang, T. J. (2014) · Lab on a Chip · doi:10.1039/c3lc90136j · Cited by 307
Marx, U. (2016) · ALTEX · doi:10.14573/altex.1603161 · Cited by 295
Liver-cell patterning lab chip: mimicking the morphology of liver lobule tissue
Ho, C.-T., Lin, R.-Z., Chen, R.-J., Chin, C.-K., Gong, S.-E., Chang, H.-Y., Peng, H.-L., Hsu, L., Yew, T.-R., Chang, S.-F., et al. (2013) · Lab on a Chip · doi:10.1039/c3lc50402f · Cited by 230
Self-citationOrgans-on-a-chip: a new tool for drug discovery
Polini, A., Prodanov, L., Bhise, N. S., Manoharan, V., Dokmeci, M. R., & Khademhosseini, A. (2014) · Expert Opinion on Drug Discovery · doi:10.1517/17460441.2014.886562 · Cited by 223
Yoon No, D., Lee, K.-H., Lee, J., & Lee, S.-H. (2015) · Lab on a Chip · doi:10.1039/c5lc00611b · Cited by 205
Ma, C., Zhao, L., Zhou, E.-M., Xu, J., Shen, S., & Wang, J. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b03869 · Cited by 145
Integrated heart/cancer on a chip to reproduce the side effects of anti-cancer drugs in vitro
Kamei, K.-I., Kato, Y., Hirai, Y., Ito, S., Satoh, J., Oka, A., Tsuchiya, T., Chen, Y., & Tabata, O. (2017) · RSC Advances · doi:10.1039/c7ra07716e · Cited by 113
The use of electric fields in tissue engineering: A review
Markx, G. H. (2008) · Organogenesis · doi:10.4161/org.5799 · Cited by 92
Beer, M., Kuppalu, N., Stefanini, M., Becker, H., Schulz, I., Manoli, S., Schuette, J., Schmees, C., Casazza, A., Stelzle, M., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-01256-8 · Cited by 75
Liver microsystems in vitro for drug response
Lee, J.-H., Ho, K.-L., & Fan, S.-K. (2019) · Journal of Biomedical Science · doi:10.1186/s12929-019-0575-0 · Cited by 33
Metadata
- Authors
- Ho, C.-T., Lin, R.-Z., Chang, W.-Y., Chang, H.-Y., & Liu, C.-H.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2006
- Volume
- 6
- Issue
- 6
- Pages
- 724
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2144869738
- Cited by
- 283
- References
- 0
- FWCI
- 10.97
- Type
- journal article
- Language
- en
- Open access
- closed
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