The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Digital microfluidics for cell-based assays
Abstract
We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. A cytotoxicity assay using Jurkat T-cells was performed using the new method, which had approximately 20 times higher sensitivity than a conventional well plate assay. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays on the microscale.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
Unger, M. A., Chou, H.-P., Thorsen, T., Scherer, A., & Quake, S. R. (2000) · Science · doi:10.1126/science.288.5463.113 · Cited by 4,010
Microfluidic Large-Scale Integration
Thorsen, T., Maerkl, S. J., & Quake, S. R. (2002) · Science · doi:10.1126/science.1076996 · Cited by 2,222
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,102
Functional hydrogel structures for autonomous flow control inside microfluidic channels
Beebe, D. J., Moore, J. S., Bauer, J. M., Yu, Q., Liu, R. H., Devadoss, C., & Jo, B.-H. (2000) · Nature · doi:10.1038/35007047 · Cited by 1,971
Electrowetting-based actuation of liquid droplets for microfluidic applications
Pollack, M. G., Fair, R. B., & Shenderov, A. D. (2000) · Applied Physics Letters · doi:10.1063/1.1308534 · Cited by 1,481
Srinivasan, V., Pamula, V. K., & Fair, R. B. (2004) · Lab on a Chip · doi:10.1039/b403341h · Cited by 1,066
Li Jeon, N., Baskaran, H., Dertinger, S. K. W., Whitesides, G. M., Van De Water, L., & Toner, M. (2002) · Nature Biotechnology · doi:10.1038/nbt712 · Cited by 917
Generation of Gradients Having Complex Shapes Using Microfluidic Networks
Dertinger, S. K. W., Chiu, D. T., Jeon, N. L., & Whitesides, G. M. (2001) · Analytical Chemistry · doi:10.1021/ac001132d · Cited by 839
Electrowetting and electrowetting-on-dielectric for microscale liquid handling
Lee, J., Moon, H., Fowler, J., Schoellhammer, T., & Kim, C.-J. (2002) · Sensors and Actuators A: Physical · doi:10.1016/s0924-4247(01)00734-8 · Cited by 652
Microfluidic Device for Single-Cell Analysis
Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A. (2003) · Analytical Chemistry · doi:10.1021/ac0340758 · Cited by 600
Self-citation
Cited by
Active droplet sorting in microfluidics: a review
Xi, H.-D., Zheng, H., Guo, W., Gañán-Calvo, A. M., Ai, Y., Tsao, C.-W., Zhou, J., Li, W., Huang, Y., Nguyen, N.-T., et al. (2017) · Lab on a Chip · doi:10.1039/c6lc01435f · Cited by 369
Microvalves and Micropumps for BioMEMS
Au, A. K., Lai, H., Utela, B. R., & Folch, A. (2011) · Micromachines · doi:10.3390/mi2020179 · Cited by 332
Digital microfluidics: a versatile tool for applications in chemistry, biology and medicine
Jebrail, M. J., Bartsch, M. S., & Patel, K. D. (2012) · Lab on a Chip · doi:10.1039/c2lc40318h · Cited by 324
Microfluidics for cell-based high throughput screening platforms—A review
Du, G., Fang, Q., & den Toonder, J. M. J. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2015.11.023 · Cited by 276
Hepatic organoids for microfluidic drug screening
Au, S. H., Chamberlain, M. D., Mahesh, S., Sefton, M. V., & Wheeler, A. R. (2014) · Lab on a Chip · doi:10.1039/c4lc00531g · Cited by 162
Self-citation
Metadata
- Authors
- Barbulovic-Nad, I., Yang, H., Park, P. S., & Wheeler, A. R.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2008
- Volume
- 8
- Issue
- 4
- Pages
- 519
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2065075903
- Cited by
- 337
- References
- 34
- FWCI
- 23.91
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Digital microfluidics for cell-based assays. https://app.cochat.ai/papers/10.1039/b717759c
- The paper
- Barbulovic-Nad, I., Yang, H., Park, P. S., & Wheeler, A. R. (2008). Digital microfluidics for cell-based assays. Lab on a Chip. https://doi.org/10.1039/b717759c
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
