Cited by 615Microfluidic and Capillary Electrophoresis Applications3D Printing in Biomedical ResearchMicrofluidic and Bio-sensing Technologies

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Fundamentals of microfluidic cell culture in controlled microenvironments

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Young, Edmond W. K., Beebe, David J.·2010·Chemical Society Reviews

DOI 10.1039/b909900jPMC PMC2967183

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Abstract

Microfluidics has the potential to revolutionize the way we approach cell biology research. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. One of the key benefits of microfluidics for basic biology is the ability to control parameters of the cell microenvironment at relevant length and time scales. Considerable progress has been made in the design and use of novel microfluidic devices for culturing cells and for subsequent treatment and analysis. With the recent pace of scientific discovery, it is becoming increasingly important to evaluate existing tools and techniques, and to synthesize fundamental concepts that would further improve the efficiency of biological research at the microscale. This tutorial review integrates fundamental principles from cell biology and local microenvironments with cell culture techniques and concepts in microfluidics. Culturing cells in microscale environments requires knowledge of multiple disciplines including physics, biochemistry, and engineering. We discuss basic concepts related to the physical and biochemical microenvironments of the cell, physicochemical properties of that microenvironment, cell culture techniques, and practical knowledge of microfluidic device design and operation. We also discuss the most recent advances in microfluidic cell culture and their implications on the future of the field. The goal is to guide new and interested researchers to the important areas and challenges facing the scientific community as we strive toward full integration of microfluidics with biology.

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Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
Unger, M., Chou, H.-P., Thorsen, T., Scherer, A., & Quake, S. R. (2000) · Science · doi:10.1126/science.288.5463.113 · Cited by 4,016
Culture of Animal Cells
Freshney, R. I. (2005) · Wiley eBooks · doi:10.1002/9780470649367 · Cited by 3,904
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
Discher, D. E., Mooney, D. J., & Zandstra, P. W. (2009) · Science · doi:10.1126/science.1171643 · Cited by 2,666
Soft Lithography in Biology and Biochemistry
Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X., & Ingber, D. E. (2001) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.3.1.335 · Cited by 2,603
Cells on chips
El-Ali, J., Sorger, P. K., & Jensen, K. F. (2006) · Nature · doi:10.1038/nature05063 · Cited by 2,105
Physics and Applications of Microfluidics in Biology
Beebe, D. J., Mensing, G. A., & Walker, G. M. (2002) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.4.112601.125916 · Cited by 1,803
Self-citation
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Pamme, N. (2005) · Lab on a Chip · doi:10.1039/b513005k · Cited by 1,141
Review on micro molding of thermoplastic polymers
Heckele, M., & Schomburg, W. K. (2003) · Journal of Micromechanics and Microengineering · doi:10.1088/0960-1317/14/3/r01 · Cited by 924
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Regehr, K. J., Domenech, M., Koepsel, J. T., Carver, K. C., Ellison-Zelski, S. J., Murphy, W. L., Schuler, L. A., Alarid, E. T., & Beebe, D. J. (2009) · Lab on a Chip · doi:10.1039/b903043c · Cited by 719
Self-citation

Show all 54 references

Research works printed but not linked: 1

Cressey, 2008, Nat. Med., 14, 1292

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Authors: Young, E. W. K., & Beebe, D. J.
Affiliations: University of Wisconsin–Madison (US)
Corresponding author: Beebe, David J.
ORCID: Young, Edmond W. K.
Beebe, David J.
Venue: Chemical Society Reviews
Publisher: Royal Society of Chemistry (RSC)
Published: 2010
Volume: 39
Issue: 3
Pages: 1036
ISSN: 0306-0012 (ISSN-L)
1460-4744
DOI: 10.1039/b909900j
PMC: PMC2967183
OpenAlex: W2092941484
Cited by: 615
References: 54
Citations by year: 2026: 30 · 2025: 35 · 2024: 29 · 2023: 32 · 2022: 39 · 2021: 35 · 2020: 42 · 2019: 38 · 2018: 60 · 2017: 38 · 2016: 41 · 2015: 29 · 2014: 45 · 2013: 44 · 2012: 35
FWCI: 3.17
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Chemical Society Reviews (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: Natural Sciences and Engineering Research Council of Canada
National Cancer Institute (R33CA137673)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
Keywords: microfluidic cell culture, cell microenvironment, microfluidic device design, cell culture, microfluidics
MeSH terms: Endothelium, Humans, Muscle, Smooth, Cell Culture Techniques, Microfluidics

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Fundamentals of microfluidic cell culture in controlled microenvironments

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