The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA)
Abstract
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a commercially available centrifugal analyzer for incubation at 37 degrees C and real-time fluorescence detection. The system was characterized with an assay for the detection of the antibiotic resistance gene mecA of Staphylococcus aureus. The limit of detection was <10 copies and time-to-result was <20 min. Microfluidic unit operations comprise storage and release of liquid reagents, reconstitution of lyophilized reagents, aliquoting the sample into < or = 30 independent reaction cavities, and mixing of reagents with the DNA samples. The foil-based cartridge was produced by blow-molding and sealed with a self-adhesive tape. The demonstrated system excels existing PCR based lab-on-a-chip platforms in terms of energy efficiency and time-to-result. Applications are suggested in the field of mobile point-of-care analysis, B-detection, or in combination with continuous monitoring systems.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
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Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
DNA Detection Using Recombination Proteins
Piepenburg, O., Williams, C. H., Stemple, D. L., & Armes, N. A. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040204 · Cited by 2,482
Self-citationMutation detection and single-molecule counting using isothermal rolling-circle amplification
Lizardi, P. M., Huang, X., Zhu, Z., Bray-Ward, P., Thomas, D. C., & Ward, D. C. (1998) · Nature Genetics · doi:10.1038/898 · Cited by 1,442
Nucleic acid sequence-based amplification
Compton, J. (1991) · Nature · doi:10.1038/350091a0 · Cited by 1,344
Microfluidic platforms for lab-on-a-chip applications
Haeberle, S., & Zengerle, R. (2007) · Lab on a Chip · doi:10.1039/b706364b · Cited by 1,124
Self-citationHelicase‐dependent isothermal DNA amplification
Vincent, M., Xu, Y., & Kong, H. (2004) · EMBO reports · doi:10.1038/sj.embor.7400200 · Cited by 877
Strand displacement amplification—an isothermal,in vitro DNA amplification technique
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Lab-on-a-chip devices for global health: Past studies and future opportunities
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Cited by
Kersting, S., Rausch, V., Bier, F. F., & von Nickisch-Rosenegk, M. (2014) · Malaria Journal · doi:10.1186/1475-2875-13-99 · Cited by 287
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
Lab-on-a-Foil: microfluidics on thin and flexible films
Focke, M., Kosse, D., Müller, C., Reinecke, H., Zengerle, R., & von Stetten, F. (2010) · Lab on a Chip · doi:10.1039/c001195a · Cited by 255
Self-citationShen, F., Davydova, E. K., Du, W., Kreutz, J. E., Piepenburg, O., & Ismagilov, R. F. (2011) · Analytical Chemistry · doi:10.1021/ac200247e · Cited by 240
Self-citation
Metadata
- Authors
- Lutz, S., Weber, P., Focke, M., Faltin, B., Hoffmann, J., Müller, C., Mark, D., Roth, G., Munday, P., Armes, N., Piepenburg, O., Zengerle, R., & von Stetten, F.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2010
- Volume
- 10
- Issue
- 7
- Pages
- 887
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2114840438
- Cited by
- 344
- References
- 24
- FWCI
- 12.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lutz, S., Weber, P., Focke, M., Faltin, B., Hoffmann, J., Müller, C., Mark, D., Roth, G., Munday, P., Armes, N., Piepenburg, O., Zengerle, R., & von Stetten, F. (2010). Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab on a Chip. https://doi.org/10.1039/b921140c
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