Cited by 95Biofuel production and bioconversionMicrobial Metabolic Engineering and BioproductionCatalysis for Biomass Conversion

d-(−)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24

Singhvi, Mamta, Joshi, Dipti, Adsul, Mukund, Varma, Anjani, Gokhale, Digambar·2010·Green Chemistry

Abstract

Lactobacillus lactis mutant RM2-24 utilizes cellobiose efficiently, converting it into D-(−)-lactic acid. Cellobiose-degrading enzyme activities were determined for whole cells, cell extracts and disrupted cells. Aryl-β-glucosidase activity was detected in whole cells and disrupted cells, suggesting that these activities are confined to the cells. The mutant produced 80 g l−1 of lactic acid from 100 g l−1 of cellobiose with 1.66 g l−1 h−1 productivity. Production of D-lactic acid from different cellulose samples was also studied. The cellulose samples at high concentration (10%) were hydrolyzed by cellulase enzyme preparation (10 FPU g−1 cellulose) derived from Penicillium janthinellum mutant EU1 generated in our own laboratory. We obtained a maximum 72% hydrolysis, yielding glucose and cellobiose as the main end products. Lactic acid was produced from these cellulose samples by simultaneous saccharification and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus lactis mutant RM2-24. A maximum lactic acid concentration of 73 g l−1 was produced from a concentration of 100 g l−1 of bagasse-derived cellulose, the highest productivity and yield being 1.52 g l−1 h−1 and 0.73 g g−1, respectively. Considering that bagasse is a waste material available in abundance, we propose to use this biomass to produce cellulose and then sugars, which can be fermented to valuable products such as ethanol and lactic acid.

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Crossref · 10.1039/b925975a

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16 of 16 references verified0 citation errors · 0 not checkable

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  • Polylactic acid (PLA) is a lactic acid derivative produced from renewable resources such as wheat, straw, corn, and sorghum, and it is completely biodegradable.

    “Polylactic acid (PLA) is a kind of lactic acid (LA) derivative produced from renewable resources such as wheat, straw, corn, and sorghum, and it is completely biodegradable [4,5].”

    From Synthesis and Biological Application of Polylactic Acid (2020) · cites this paper as [5]

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Authors
Singhvi, M., Joshi, D., Adsul, M., Varma, A., & Gokhale, D.
Affiliations
National Chemical Laboratory (IN)
Corresponding author
Gokhale, Digambar
Venue
Green Chemistry
Published
2010
Volume
12
Issue
6
Pages
1106
ISSN
  • 1463-9262 (ISSN-L)
  • 1463-9270
OpenAlex
W2144737489
Cited by
95
References
16
Citations by year
2026: 2 · 2025: 4 · 2024: 7 · 2023: 8 · 2022: 8 · 2021: 2 · 2020: 9 · 2019: 5 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 4 · 2014: 7 · 2013: 8 · 2012: 6
FWCI
3.59
Type
journal article
Language
en
Open access
closed
Locations
Green Chemistry (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Engineering › Biomedical Engineering › Catalysis for Biomass Conversion
Keywords
D-lactic acid production, cellobiose utilization, cellulose hydrolysis, simultaneous saccharification and fermentation, sugarcane bagasse, lignocellulosic biomass conversion, lactic acid fermentation

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