Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip
Abstract
We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere® suspension at 5.6 × 10(4) beads µl(-1) which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB.
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Ryu et al. demonstrated a compact, inexpensive, and practical fluorescence detection system for lab-on-a-chip applications that can detect the cardiac markers myoglobin and CK-MB from human plasma.
“Interesting and useful is also the research by Ryu et al. [36] where they demonstrated a compact, inexpensive, and practical fluorescence detection system for lab-on-a-chip applications, consisting of 501 nm commercially available InGaN LED, an organic or silicon photodiode, absorptive dye-coated color filters, polarizers, and an injected polystyrene microfluidic chip.”
From The Current State of Optical Sensors in Medical Wearables (2022) · cites this paper as [36]
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Wearable Organic Optoelectronic Sensors for Medicine
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“[36] where they demonstrated a compact, inexpensive, and practical fluorescence detection system for lab-on-a-chip applications, consisting of 501 nm commercially available InGaN LED, an organic or silicon photodiode, absorptive dye-coated color filters, polarizers, and an injected polystyrene microfluidic chip.”
Background
- Authors
- Ryu, G., Huang, J., Hofmann, O., Walshe, C. A., Sze, J. Y. Y., McClean, G. D., Mosley, A., Rattle, S. J., deMello, J. C., deMello, A. J., & Bradley, D. D. C.
- Affiliations
- Imperial College London (GB)
- Corresponding author
- Rattle, Simon J.
- Bradley, Donal D. C.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2011
- Volume
- 11
- Issue
- 9
- Pages
- 1664
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2082891648
- Cited by
- 93
- References
- 30
- Citations by year
- 2025: 6 · 2024: 3 · 2023: 6 · 2022: 9 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 7 · 2016: 4 · 2015: 6 · 2014: 11 · 2013: 6 · 2012: 6
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Keywords
- microfluidics, photodiode detector, polarization, fluorescence immunoassay, myoglobin, CK-MB, cardiac biomarkers, detection limit, human plasma
- MeSH terms
- Fluorescent Antibody Technique, Humans, Myoglobin, Sensitivity and Specificity, Biomarkers, Point-of-Care Systems, Microfluidic Analytical Techniques, Creatine Kinase, MB Form, Lab-On-A-Chip Devices
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