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A specific and sensitive method for detection of hypochlorous acid for the imaging of microbe-induced HOCl production
Abstract
A specific and sensitive fluorescence-based method was developed for the imaging of microbe-induced HOCl production. Furthermore, we demonstrate dual oxidase (DUOX)-mediated HOCl generation in the mucosa of live animals providing a novel insight into mucosal innate immunity.
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“HOCl has superior bactericidal characteristics to superoxide [177].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [177]
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Metadata
- Authors
- Chen, X., Lee, K.-A., Ha, E.-M., Lee, K. M., Seo, Y. Y., Choi, H. K., Kim, H. N., Kim, M. J., Cho, C.-S., Lee, S. Y., Lee, W.-J., & Yoon, J.
- Affiliations
- Ewha Womans University (KR)
- Daegu Catholic University (KR)
- Seoul National University (KR)
- Corresponding author
- Lee, Won-Jae
- Yoon, Juyoung
- ORCID
- Venue
- Chemical Communications
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2011
- Volume
- 47
- Issue
- 15
- Pages
- 4373
- ISSN
- 1359-7345 (ISSN-L)
- 1364-548X
- OpenAlex
- W2048840387
- Cited by
- 246
- References
- 42
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 11 · 2021: 11 · 2020: 9 · 2019: 14 · 2018: 22 · 2017: 22 · 2016: 34 · 2015: 26 · 2014: 24 · 2013: 36 · 2012: 18
- FWCI
- 12.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- hypochlorous acid, fluorescence detection, microbial infection, dual oxidase, mucosal innate immunity, live animal imaging, reactive oxygen species
- MeSH terms
- Animals, Cell Survival, Drosophila melanogaster, Fungi, Humans, Hypochlorous Acid, Immunity, Innate, Intestinal Mucosa, Neutrophils, Phagosomes, Rhodamines, Bacterial Physiological Phenomena, NADPH Oxidases, Molecular Imaging
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