Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; the influence of PpIX concentration and illumination parameters
Open accessAbstract
Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.
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More PpIX production and better tissue oxygenation occurring during the 2 h period between two illuminations likely increase tumor cell sensitivity to the second treatment.
“Although the detailed mechanism underlying increased response to double-illumination PDT remains unknown, more PpIX production and better tissue oxygenation occurring during the 2 h period between two illuminations likely increase tumor cell sensitivity to the second treatment [68,69].”
From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [68]
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- Authors
- de Bruijn, H. S., Casas, A. G., Di Venosa, G., Gandara, L., Sterenborg, H. J. C. M., Batlle, A., & Robinson, D. J.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Erasmus MC (NL)
- Corresponding author
- Robinson, Dominic J.
- Venue
- Photochemical & Photobiological Sciences
- Published
- 2013-02
- Volume
- 12
- Issue
- 2
- Pages
- 241-245
- ISSN
- 1474-905X (ISSN-L)
- 1474-9092
- OpenAlex
- W2003707844
- Cited by
- 31
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2019: 3 · 2018: 2 · 2016: 9 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Photochemical & Photobiological Sciences (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Conicet (submittedVersion, cc-by-nc-sa)
- Funders
- KWF Kankerbestrijding
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Keywords
- photodynamic therapy, 5-aminolevulinic acid, protoporphyrin IX, non-melanoma skin cancer, sublethal damage, cell survival, light dose
- MeSH terms
- Aminolevulinic Acid, Animals, Cell Line, Cell Survival, Dose-Response Relationship, Drug, Light, Photochemotherapy, Structure-Activity Relationship, Photosensitizing Agents, Mice
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