The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
A highly selective turn-on fluorescent probe for iron(ii) to visualize labile iron in living cells
Abstract
Although labile iron plays critical roles in diverse biological processes in living cells, the physiological and pathophysiological functions of iron have not been sufficiently explored, partially due to a lack of methods for visualizing intracellular labile iron. In this edge article, we present a novel turn-on fluorescent probe (RhoNox-1) for the selective detection of Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations and electrochemical studies revealed that fluorescence quenching of RhoNox-1 occurred in physiological conditions, which was attributed to non-radiative deactivation of the excited state of tertiary amine N-oxide substituted xanthene involving a twisted internal charge transfer (TICT) process and partially due to photo-induced electron transfer (PET) from the N-oxide group. RhoNox-1 showed significant enhancement of the fluorescence signal in Fe2+-loaded cells via selective Fe2+-mediated deoxygenation of the N-oxide group and also successfully detected basal and endogenous labile Fe2+ in living cells.
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Cites
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Adachi, T., Tanaka, H., Nonomura, S., Hara, H., Kondo, S.-I., & Hori, M. (2015) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.11.014 · Cited by 334
Iron and thiol redox signaling in cancer: an exquisite balance to escape ferroptosis
Toyokuni, S., Ito, F., Yamashita, K., Okazaki, Y., & Akatsuka, S. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.04.024 · Cited by 252
Grover, K., Koblova, A., Pezacki, A. T., Chang, C. J., & New, E. J. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00819 · Cited by 129
Toyokuni, S. (2016) · Pathology International · doi:10.1111/pin.12396 · Cited by 111
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Hirayama, T. (2019) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.07.004 · Cited by 79
Metadata
- Authors
- Hirayama, T., Okuda, K., & Nagasawa, H.
- Venue
- Chemical Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2013
- Volume
- 4
- Issue
- 3
- Pages
- 1250
- ISSN
- 2041-6520 (ISSN-L)
- 2041-6539
- OpenAlex
- W1974917614
- Cited by
- 296
- References
- 62
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- closed
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