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Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general
Open accessAbstract
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.
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The adsorption of gases on plane surfaces of glass, mica and platinum
Langmuir, I. (1918) · Journal of the American Chemical Society · doi:10.1021/ja02242a004 · Cited by 23,242
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Metadata
- Authors
- Zhang, S., Garcia-D'Angeli, A., Brennan, J. P., & Huo, Q.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2014
- Volume
- 139
- Issue
- 2
- Pages
- 439-445
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- OpenAlex
- W2003544627
- Cited by
- 219
- References
- 1
- FWCI
- 2.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Zhang, S., Garcia-D'Angeli, A., Brennan, J. P., & Huo, Q. (2014). Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general. The Analyst. https://doi.org/10.1039/c3an01835k
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