Cited by 306Photochemistry and Electron Transfer StudiesSpectroscopy and Quantum Chemical StudiesMolecular Sensors and Ion Detection

Universal quenching of common fluorescent probes by water and alcohols

Open access
Maillard, Jimmy, Klehs, Kathrin, Rumble, Christopher, Vauthey, Eric, Heilemann, Mike, Fürstenberg, Alexandre·2021·Chemical Science

Abstract

Overtones and combinations of O–H vibrations in the solvent efficiently quench red-emitting fluorophores by resonant energy transfer.

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Integrity signals

Registry

Crossref · 10.1039/d0sc05431c

Cites

90 of 90 references verified0 citation errors · 0 not checkable

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  • In the case of polymethine dyes, water can reduce fluorescence quantum yield by up to threefold.

    “In the case of polymethine dyes, water can reduce Φfl by up to threefold.76”

    From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [76]

    The Results (page not specified) include cyanine dyes and report fluorescence quantum yields up to 205% higher in D₂O than in H₂O—approximately a threefold difference. The Discussion (page not specified) reports enhancements up to 3.1-fold for red-emitting dyes, but our copy does not establish that this maximum was measured specifically for polymethine dyes. (LLM, conf=0.78)

    Experimental — accuracy not yet measured

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Cites

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Research works printed but not linked: 1

  1. Nau W. M. Hennig A. Koner A. L. Springer Ser. Fluoresc. 2008 4 185 211

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Authors
Maillard, J., Klehs, K., Rumble, C., Vauthey, E., Heilemann, M., & Fürstenberg, A.
Affiliations
  • University of Geneva (CH)
  • Goethe University Frankfurt (DE)
Corresponding author
  • Heilemann, Mike
  • Fürstenberg, Alexandre
Venue
Chemical Science
Published
2021
Volume
12
Issue
4
Pages
1352-1362
ISSN
  • 2041-6520 (ISSN-L)
  • 2041-6539
OpenAlex
W3109218211
Cited by
306
References
90
Citations by year
2026: 57 · 2025: 67 · 2024: 75 · 2023: 57 · 2022: 35 · 2021: 15
FWCI
15.55
Type
journal article
Language
en
Open access
Locations
  • Chemical Science (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
Université de Genève
Topics
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
Keywords
fluorescence quenching, red-emitting fluorophores, water, alcohol chemistry, solvent effects, vibrational overtones, combination bands, resonance energy transfer, fluorescent probes

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