Universal quenching of common fluorescent probes by water and alcohols
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Overtones and combinations of O–H vibrations in the solvent efficiently quench red-emitting fluorophores by resonant energy transfer.
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In the case of polymethine dyes, water can reduce fluorescence quantum yield by up to threefold.
“In the case of polymethine dyes, water can reduce Φfl by up to threefold.76”
From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [76]
The Results (page not specified) include cyanine dyes and report fluorescence quantum yields up to 205% higher in D₂O than in H₂O—approximately a threefold difference. The Discussion (page not specified) reports enhancements up to 3.1-fold for red-emitting dyes, but our copy does not establish that this maximum was measured specifically for polymethine dyes. (LLM, conf=0.78)
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Self-citationA Dual‐Emissive DNA‐Templated Silver Nanocluster with Near‐Infrared I and II Emission
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- Authors
- Maillard, J., Klehs, K., Rumble, C., Vauthey, E., Heilemann, M., & Fürstenberg, A.
- Affiliations
- University of Geneva (CH)
- Goethe University Frankfurt (DE)
- Corresponding author
- Heilemann, Mike
- Fürstenberg, Alexandre
- Venue
- Chemical Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2021
- Volume
- 12
- Issue
- 4
- Pages
- 1352-1362
- ISSN
- 2041-6520 (ISSN-L)
- 2041-6539
- PMC
- PMC8179231
- OpenAlex
- W3109218211
- Cited by
- 306
- References
- 90
- Citations by year
- 2026: 57 · 2025: 67 · 2024: 75 · 2023: 57 · 2022: 35 · 2021: 15
- FWCI
- 15.55
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Chemical Science (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- Université de Genève
- Topics
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Keywords
- fluorescence quenching, red-emitting fluorophores, water, alcohol chemistry, solvent effects, vibrational overtones, combination bands, resonance energy transfer, fluorescent probes
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