Cited by 10Extracellular vesicles in diseaseAdvanced Proteomics Techniques and ApplicationsNanoparticle-Based Drug Delivery

A new integrated method for tissue extracellular vesicle enrichment and proteome profiling

Open access
Zhang, Miaomiao, Liu, Tong, Du, Zhuokun, Li, Hang, Qin, Weijie·2022·RSC Advances

Abstract

Extracellular vesicles were successfully extracted from mouse liver tissue using kit combined with TiO 2 .

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1039/d2ra06185f

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • A substantial quantity of EVs is needed to address CNS diseases, particularly in cases where the affected region is sizable.

    “Furthermore, a substantial quantity of EVs is needed to address CNS diseases, particularly in cases where the affected region is sizable.”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [330]

    The Introduction discusses isolating EVs from solid tissues for tumor research, and the Results examine EVs from liver tissue in mice with hepatocellular carcinoma. Searches for CNS disease treatment, EV quantities, and affected-region size found no passage addressing the claimed need for a substantial quantity of EVs. (LLM, conf=0.75)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 41

  1. György B. Szabó T. G. Pásztói M. Pál Z. Misják P. Aradi B. László V. Pállinger É. Pap E. Kittel Á. Nagy G. Falus A. Buzás E. I. Cell. Mol. Life Sci. 2011 68 2667 2688 21560073 PMC3142546
  2. New Technologies for Analysis of Extracellular Vesicles – PMC, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029891/, accessed 26 May, 2022
  3. Sun Y. Liu S. Qiao Z. Shang Z. Xia Z. Niu X. Qian L. Zhang Y. Fan L. Cao C.-X. Xiao H. Anal. Chim. Acta 2017 982 84 95 28734369
  4. Melo S. A. Luecke L. B. Kahlert C. Fernandez A. F. Gammon S. T. Kaye J. LeBleu V. S. Mittendorf E. A. Weitz J. Rahbari N. Reissfelder C. Pilarsky C. Fraga M. F. Piwnica-Worms D. Kalluri R. Nature 2015 523 177 182 26106858 PMC4825698
  5. Chen T. Xi Q.-Y. Ye R.-S. Cheng X. Qi Q.-E. Wang S.-B. Shu G. Wang L.-N. Zhu X.-T. Jiang Q.-Y. Zhang Y.-L. BMC Genomics 2014 15 100 24499489 PMC4008308
  6. Pisitkun T. Shen R.-F. Knepper M. A. Proc. Natl. Acad. Sci. U. S. A. 2004 101 13368 13373 15326289 PMC516573
  7. Simons M. Raposo G. Curr. Opin. Cell Biol. 2009 21 575 581 19442504
  8. Clayton A. Turkes A. Dewitt S. Steadman R. Mason M. D. Hallett M. B. FASEB J. 2004 18 977 979 15059973
  9. Valadi H. Ekström K. Bossios A. Sjöstrand M. Lee J. J. Lötvall J. O. Nat. Cell Biol. 2007 9 654 659 17486113
  10. Kalluri R. J. Clin. Invest. 2016 126 1208 1215 27035812 PMC4811149
  11. Belov L. Matic K. J. Hallal S. Best O. G. Mulligan S. P. Christopherson R. I. J. Extracell. Vesicles 2016 5 25355 27086589 PMC4834364
  12. Li S. Man Q. Gao X. Lin H. Wang J. Su F. Wang H. Bu L. Liu B. Chen G. J. Extracell. Vesicles 2021 10 e12175 34918479 PMC8678102
  13. Leung L. L. Riaz M. K. Qu X. Chan J. Meehan K. Semin. Cancer Biol. 2021 74 3 23 33460766
  14. Luga V. Zhang L. Viloria-Petit A. M. Ogunjimi A. A. Inanlou M. R. Chiu E. Buchanan M. Hosein A. N. Basik M. Wrana J. L. Cell 2012 151 1542 1556 23260141
  15. Torre L. A. Bray F. Siegel R. L. Ferlay J. Lortet-Tieulent J. Jemal A. Cancer C. A. J. Clin. 2015 65 87 108 25651787
  16. Qu Z. Wu J. Wu J. Luo D. Jiang C. Ding Y. J. Exp. Clin. Cancer Res. 2016 35 159 27716356 PMC5045585
  17. Grohmann M. Wiede F. Dodd G. T. Gurzov E. N. Ooi G. J. Butt T. Rasmiena A. A. Kaur S. Gulati T. Goh P. K. Treloar A. E. Archer S. Brown W. A. Muller M. Watt M. J. Ohara O. McLean C. A. Tiganis T. Cell 2018 175 1289 1306 30454647 PMC6242467
  18. Greening D. W. Gopal S. K. Mathias R. A. Liu L. Sheng J. Zhu H.-J. Simpson R. J. Semin. Cell Dev. Biol. 2015 40 60 71 25721809
  19. Ludwig N. Whiteside T. L. Expert Opin. Ther. Targets 2018 22 409 417 29634426 PMC6126896
  20. Shao C. Yang F. Miao S. Liu W. Wang C. Shu Y. Shen H. Mol. Cancer 2018 17 120 30098600 PMC6087002
  21. Xue X. Wang X. Zhao Y. Hu R. Qin L. Biochem. Biophys. Res. Commun. 2018 502 515 521 29859935
  22. Takahashi K. Yan I. K. Wood J. Haga H. Patel T. Mol. Cancer Res. 2014 12 1377 1387 24874432 PMC4201956
  23. Huang Y. Cheng L. Turchinovich A. Mahairaki V. Troncoso J. C. Pletniková O. Haughey N. J. Vella L. J. Hill A. F. Zheng L. Witwer K. W. J. Extracell. Vesicles 2020 9 1785746 32944174 PMC7480582
  24. Perez-Gonzalez R. Gauthier S. A. Kumar A. Levy E. J. Biol. Chem. 2012 287 43108 43115 23129776 PMC3522305
  25. Chen B.-Y. Sung C. W.-H. Chen C. Cheng C.-M. Lin D. P.-C. Huang C.-T. Hsu M.-Y. Clin. Chim. Acta 2019 493 14 19 30797770
  26. Caradec J. Kharmate G. Hosseini-Beheshti E. Adomat H. Gleave M. Guns E. Clin. Biochem. 2014 47 1286 1292 24956264
  27. Mol E. A. Goumans M.-J. Doevendans P. A. Sluijter J. P. G. Vader P. Nanomedicine Nanotechnol. Biol. Med. 2017 13 2061 2065 28365418
  28. Soares Martins T. Catita J. Martins Rosa I. da Cruz E Silva O. A. B. Henriques A. G. PloS One 2018 13 e0198820 29889903 PMC5995457
  29. Cao F. Gao Y. Chu Q. Wu Q. Zhao L. Lan T. Zhao L. Electrophoresis 2019 40 3092 3098 31621929
  30. Uehara T. Pogribny I. P. Rusyn I. Curr. Protoc. Pharmacol. 2014 66 14.30.1 14.30.10 25181010 PMC4214366
  31. Coughlan C. Bruce K. D. Burgy O. Boyd T. D. Michel C. R. Garcia-Perez J. E. Adame V. Anton P. Bettcher B. M. Chial H. J. Königshoff M. Hsieh E. W. Y. Graner M. Potter H. Curr. Protoc. Cell Biol. 2020 88 e110 32633898 PMC8088761
  32. Crescitelli R. Lässer C. Lötvall J. Nat. Protoc. 2021 16 1548 1580 33495626
  33. Robinson M. D. McCarthy D. J. Smyth G. K. Bioinformatics 2010 26 139 140 19910308 PMC2796818
  34. Huang D. W. Sherman B. T. Lempicki R. A. Nat. Protoc. 2009 4 44 57 19131956
  35. Fang X. Chen C. Liu B. Ma Z. Hu F. Li H. Gu H. Xu H. Acta Biomater. 2021 124 336 347 33578055
  36. Lobb R. J. Becker M. Wen Wen S. Wong C. S. F. Wiegmans A. P. Leimgruber A. Möller A. J. Extracell. Vesicles 2015 4 27031 26194179 PMC4507751
  37. Reshi Q. U. A., Hasan M. M., Dissanayake K. and Fazeli A., in Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols, ed. T. A. L. Brevini, A. Fazeli and K. Turksen, Springer US, New York, NY, 2021, pp. 201–206
  38. Paulo J. A. Lee L. S. Wu B. Repas K. Banks P. A. Conwell D. L. Steen H. Electrophoresis 2010 31 2377 2387 20589857 PMC3019099
  39. Keerthikumar S. Chisanga D. Ariyaratne D. Saffar H. A. Anand S. Zhao K. Samuel M. Pathan M. Jois M. Chilamkurti N. Gangoda L. Mathivanan S. J. Mol. Biol. 2016 428 688 692 26434508 PMC4783248
  40. Li M. Jin C. Xu M. Zhou L. Li D. Yin Y. Cell Commun. Signal 2017 15 52 29246230 PMC5732395
  41. Tong M. Fung T.-M. Luk S. T. Ng K.-Y. Lee T. K. Lin C.-H. Yam J. W. Chan K. W. Ng F. Zheng B.-J. Yuan Y.-F. Xie D. Lo C.-M. Man K. Guan X.-Y. Ma S. Stem Cell Rep. 2015 5 45 59 PMC4618447 26095609

Cited by

Sorted by citations

Authors
Zhang, M., Liu, T., Du, Z., Li, H., & Qin, W.
Affiliations
  • Qingdao University (CN)
  • Beijing Proteome Research Center (CN)
Corresponding author
  • Li, Hang
  • Qin, Weijie
Venue
RSC Advances
Published
2022
Volume
12
Issue
51
Pages
33409-33418
ISSN
2046-2069 (ISSN-L)
OpenAlex
W4310334978
Cited by
10
References
42
Citations by year
2026: 4 · 2025: 1 · 2024: 3 · 2023: 2
FWCI
0.49
Type
journal article
Language
en
Open access
Locations
  • RSC Advances (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (32088101, 22074158, 21904008)
  • National Key Research and Development Program of China (2021YFA1302604, 2021YFA1301601)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords
extracellular vesicles, proteomic profiling, hepatocellular carcinoma, liver tissue, differentially expressed proteins, biomarker discovery, drug screening

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review