Towards high atom economy in whole-cell redox biocatalysis: Up-scaling light-driven cyanobacterial ene-reductions in a flat panel photobioreactor
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-Factor of 203 including water reveals that the volumetric yield and water required for cultivation are crucial for the sustainability. In summary, our results point out key factors for the sustainability of light-driven whole-cell biotransformations, and provide a solid basis for future optimisation and up-scale campaigns of photosynthesis-driven bioproduction.
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Variations in light intensity and photosynthetic activity can impact intracellular levels of cofactors such as NADPH, ATP, flavins, and metal ions.
“Since cyanobacterial metabolism is closely linked to photosynthetic energy production, variations in light intensity and photosynthetic activity can impact the intracellular levels of these cofactors [79,158].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [158]
The Introduction (page unavailable) says cyanobacteria use light to regenerate NADPH and ATP through photosynthetic electron transfer, supporting the link between light and those cofactors. It identifies flavin mononucleotide as an ene-reductase cofactor, but does not say that light or photosynthetic activity changes intracellular flavin or metal-ion levels. (LLM, conf=0.86)
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- Authors
- Grimm, H. C., Erlsbacher, P., Medipally, H., Malihan-Yap, L., Sovic, L., Zöhrer, J., Kosourov, S. N., Allahverdiyeva, Y., Paul, C. E., & Kourist, R.
- Affiliations
- Graz University of Technology (AT)
- Institute for Molecular Biotechnology, TU Graz, Petersgasse 14/1, A-8010, Graz, Austria
- University of Turku (FI)
- Delft University of Technology (NL)
- Corresponding author
- Grimm, Hanna C.
- Kourist, Robert
- ORCID
- Venue
- Green Chemistry
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2025
- Volume
- 27
- Issue
- 11
- Pages
- 2907-2920
- ISSN
- 1463-9262 (ISSN-L)
- 1463-9270
- OpenAlex
- W4406377011
- Cited by
- 13
- References
- 75
- Citations by year
- 2026: 9 · 2025: 4
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Green Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- UTUPub (University of Turku) (submittedVersion)
- Funders
- Horizon 2020 Framework Programme (899576)
- Austrian Science Fund (P 36614-B)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- whole-cell biocatalysis, cyanobacteria, flat panel photobioreactor, atom economy, volumetric yield, water consumption, upscaling
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