The DOI matches its Crossref record; 34 of the 37 references it cites were found in a registry or an index.
AMP-activated protein kinase: an ultrasensitive system for monitoring cellular energy charge
Open accessAbstract
The AMP-activated protein kinase cascade is activated by elevation of AMP and depression of ATP when cellular energy charge is compromised, leading to inhibition of anabolic pathways and activation of catabolic pathways. Here we show that the system responds in intact cells in an ultrasensitive manner over a critical range of nucleotide concentrations, in that only a 6-fold increase in activating nucleotide is required in order for the maximal activity of the kinase to progress from 10% to 90%, equivalent to a co-operative system with a Hill coefficient (h) of 2.5. Modelling suggests that this sensitivity arises from two features of the system: (i) AMP acts at multiple steps in the cascade (multistep sensitivity); and (ii) the upstream kinase is initially saturated with the downstream kinase (zero-order ultrasensitivity).
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Cited by
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Hawley, S. A., Pan, D. A., Mustard, K. J., Ross, L., Bain, J., Edelman, A. M., Frenguelli, B. G., & Hardie, D. G. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.05.009 · Cited by 1,589
Self-citationKinaan, M., Ding, H., & Triggle, C. R. (2015) · Medical Principles and Practice · doi:10.1159/000381643 · Cited by 1,515
AMP-Activated Protein Kinase in Metabolic Control and Insulin Signaling
Towler, M. C., & Hardie, D. G. (2007) · Circulation Research · doi:10.1161/01.res.0000256090.42690.05 · Cited by 1,262
AMPK: Sensing Glucose as well as Cellular Energy Status
Lin, S., & Hardie, D. G. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.10.009 · Cited by 1,198
The AMP-activated protein kinase pathway – new players upstream and downstream
Hardie, D. G. (2004) · Journal of Cell Science · doi:10.1242/jcs.01540 · Cited by 1,163
Metadata
- Authors
- HARDIE, D. G., SALT, I. P., HAWLEY, S. A., & DAVIES, S. P.
- Affiliations
- University of Dundee (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1999-03-08
- Volume
- 338
- Issue
- 3
- Pages
- 717-722
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1220108
- OpenAlex
- W2014670133
- Cited by
- 362
- References
- 37
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 5 · 2023: 4 · 2022: 5 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 13 · 2017: 7 · 2016: 12 · 2015: 15 · 2014: 16 · 2013: 17 · 2012: 20
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- York University
- University of Bristol
- University at Buffalo
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- AMPK, ultrasensitivity, AMP, ATP, Hill coefficient, cooperative activation, energy homeostasis
- MeSH terms
- Amino Acid Sequence, Cell Line, Energy Metabolism, Kinetics, Models, Biological, Molecular Sequence Data, Multienzyme Complexes, Sensitivity and Specificity, Protein Serine-Threonine Kinases, AMP-Activated Protein Kinases
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- CoChat. (2026). Citation check: AMP-activated protein kinase: an ultrasensitive system for monitoring cellular energy charge. https://app.cochat.ai/papers/10.1042/bj3380717
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- HARDIE, D. G., SALT, I. P., HAWLEY, S. A., & DAVIES, S. P. (1999). AMP-activated protein kinase: an ultrasensitive system for monitoring cellular energy charge. Biochemical Journal. https://doi.org/10.1042/bj3380717
