The DOI matches its OpenAlex record; 3 of the 3 references it cites were found in a registry or an index.
Impaired nitric oxide production in coronary endothelial cells of the spontaneously diabetic BB rat is due to tetrahydrobiopterin deficiency
Open accessAbstract
Endothelial cells (EC) from diabetic BioBreeding (BB) rats have an impaired ability to produce NO. This deficiency is not due to a defect in the constitutive isoform of NO synthase in EC (ecNOS) or alterations in intracellular calcium, calmodulin, NADPH or arginine levels. Instead, ecNOS cannot produce sufficient NO because of a deficiency in tetrahydrobiopterin (BH(4)), a cofactor necessary for enzyme activity. EC from diabetic rats exhibited only 12% of the BH(4) levels found in EC from normal animals or diabetes-prone animals which did not develop disease. As a result, NO synthesis by EC of diabetic rats was only 18% of that for normal animals. Increasing BH(4) levels with sepiapterin increased NO production, suggesting that BH(4) deficiency is a metabolic basis for impaired endothelial NO synthesis in diabetic BB rats. This deficiency is due to decreased activity of GTP-cyclohydrolase I, the first and rate-limiting enzyme in the de novo biosynthesis of BH(4). GTP-cyclohydrolase activity was low because of a decreased expression of the protein in the diabetic cells.
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Cites
Arginine metabolism: nitric oxide and beyond
WU, G., & MORRIS, S. M. (1998) · Biochemical Journal · doi:10.1042/bj3360001 · Cited by 2,811
Self-citationVoyta, J. C., Via, D. P., Butterfield, C. E., & Zetter, B. R. (1984) · The Journal of cell biology · doi:10.1083/jcb.99.6.2034 · Cited by 1,239
Werner-Felmayer, G., Werner, E. R., Fuchs, D., Hausen, A., Reibnegger, G., Schmidt, K., Weiss, G., & Wachter, H. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53931-4 · Cited by 263
Cited by
Oxidative stress and diabetic cardiovascular complications
Jay, D., Hitomi, H., & Griendling, K. K. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.06.018 · Cited by 464
Janaszak-Jasiecka, A., Płoska, A., Wierońska, J. M., Dobrucki, L. W., & Kalinowski, L. (2023) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-023-00423-2 · Cited by 358
Vascular endothelial dysfunction, a major mediator in diabetic cardiomyopathy
Knapp, M., Tu, X., & Wu, R. (2019) · Acta Pharmacologica Sinica · doi:10.1038/s41401-018-0042-6 · Cited by 328
Regulation of obesity and insulin resistance by nitric oxide
Sansbury, B. E., & Hill, B. G. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.05.016 · Cited by 274
Pathophysiology of the Diabetic Kidney
Vallon, V., & Komers, R. (2011) · Comprehensive Physiology · doi:10.1002/cphy.c100049 · Cited by 259
Metadata
- Authors
- Meininger, C. J., MARINOS, R. S., Hatakeyama, K., Martı́nez–Zaguilán, R., Rojas, J. D., Kelly, K. A., & Wu, G.
- Venue
- Biochemical Journal
- Publisher
- Portland Press
- Published
- 2000-06-26
- Volume
- 349
- Issue
- 1
- Pages
- 353-353
- ISSN
- 0264-6021 (ISSN-L)
- OpenAlex
- W2048574237
- Cited by
- 189
- References
- 3
- FWCI
- 6.80
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Meininger, C. J., MARINOS, R. S., Hatakeyama, K., Martı́nez–Zaguilán, R., Rojas, J. D., Kelly, K. A., & Wu, G. (2000). Impaired nitric oxide production in coronary endothelial cells of the spontaneously diabetic BB rat is due to tetrahydrobiopterin deficiency. Biochemical Journal. https://doi.org/10.1042/0264-6021%3A3490353
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