Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.
Open accessAbstract
Acetylated-low density lipoprotein (Ac-LDL) is taken up by macrophages and endothelial cells via the "scavenger cell pathway" of LDL metabolism. In this report, aortic and microvascular endothelial cells internalized and degraded 7-15 times more [125I]-Ac-LDL than did smooth muscle cells or pericytes. Bound [125I]-Ac-LDL was displaced by unlabeled Ac-LDL, but not unmodified LDL. The ability to identify endothelial cells based on their increased metabolism of Ac-LDL was examined using Ac-LDL labeled with the fluorescent probe 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (Dil-Ac-LDL). When cells were incubated with 10 micrograms/ml Dil-Ac-LDL for 4 h at 37 degrees C and subsequently examined by fluorescence microscopy, capillary and aortic endothelial cells were brilliantly fluorescent whereas the fluorescent intensity of retinal pericytes and smooth muscle cells was only slightly above background levels. Dil-Ac-LDL at the concentration used for labeling cells had no effect on endothelial cell growth rate. When primary cultures of bovine adrenal capillary cells were labeled with 10 micrograms/ml of Dil-Ac-LDL for 4 h at 37 degrees C, then trypsinized and subjected to fluorescence-activated cell sorting, pure cultures of capillary endothelial cells could be obtained. Utilizing this method, large numbers of early passage microvascular endothelial cells can be obtained in significantly less time than with conventional methods.
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Havel, R. J., Eder, H. A., & Bragdon, J. H. (1955) · Journal of Clinical Investigation · doi:10.1172/jci103182 · Cited by 8,756 · see citing works
Goldstein, J. L., Ho, Y. K., Basu, S. K., & Brown, M. S. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.333 · Cited by 2,447 · see citing works
Ross, R. (1971) · The Journal of Cell Biology · doi:10.1083/jcb.50.1.172 · Cited by 1,573 · see citing works
Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial Cells
JAFFE, E. A., Hoyer, L. W., & Nachman, R. L. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107471 · Cited by 1,291 · see citing works
Basu, S. K., Goldstein, J. L., Anderson, G. W., & Brown, M. S. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.9.3178 · Cited by 1,021 · see citing works
Fogelman, A. M., Shechter, I., Seager, J., Hokom, M. M., CHILD, J. S., & Edwards, P. A. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.4.2214 · Cited by 849 · see citing works
Long-term culture of capillary endothelial cells.
Folkman, J., Haudenschild, C. C., & Zetter, B. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.10.5217 · Cited by 766 · see citing works
Self-citationCultured endothelial cells produce heparinlike inhibitor of smooth muscle cell growth
Castellot, J. J., Addonizio, M., ROSENBERG, R. D., & Karnovsky, M. J. (1981) · The Journal of Cell Biology · doi:10.1083/jcb.90.2.372 · Cited by 679 · see citing works
Brown, M. S., Basu, S. K., Falck, J. R., Ho, Y. K., & Goldstein, J. L. (1980) · Journal of Supramolecular Structure · doi:10.1002/jss.400130107 · Cited by 528 · see citing works
Antihemophilic Factor Antigen. LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPY
Hoyer, L. W., Santos, R. P. D. L., & Hoyer, J. R. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107469 · Cited by 442 · see citing works
Nagelkerke, J. F., Barto, K. P., & van Berkel, T. J. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44160-3 · Cited by 392 · see citing works
Pitas, R. E., Innerarity, T. L., Weinstein, J. N., & Mahley, R. W. (1981) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.1.3.177 · Cited by 389 · see citing works
Ryan, U., Ryan, J. W., Whitaker, C., et al. (1976) · Tissue and Cell · doi:10.1016/0040-8166(76)90025-2 · Cited by 351 · see citing works
Atherosclerosis: The low-density lipoprotein receptor hypothesis
Goldstein, J. L., & Brown, M. S. (1977) · Metabolism · doi:10.1016/0026-0495(77)90119-6 · Cited by 337 · see citing works
Mahley, R. W., Innerarity, T. L., Weisgraber, K. H., & Oh, S. Y. (1979) · Journal of Clinical Investigation · doi:10.1172/jci109518 · Cited by 316 · see citing works
Migration of capillary endothelial cells is stimulated by tumour-derived factors
Zetter, B. R. (1980) · Nature · doi:10.1038/285041a0 · Cited by 226 · see citing works
Self-citationStein, O. T., & Stein, Y. (1980) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(80)90155-1 · Cited by 212 · see citing works
Johnson, A. R., & Erdös, E. G. (1977) · Journal of Clinical Investigation · doi:10.1172/jci108687 · Cited by 202 · see citing works
Platelet-dependent stimulation of prostacyclin synthesis by platelet-derived growth factor
Coughlin, S. R., Moskowitz, M. A., Zetter, B. R., et al. (1980) · Nature · doi:10.1038/288600a0 · Cited by 185 · see citing works
Self-citationPitas, R. E., Innerarity, T. L., & Mahley, R. W. (1983) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.3.1.2 · Cited by 147 · see citing works
Multistation multiparameter flow cytometry: A critical review and rationale
Shapiro, H. M. (1983) · Cytometry · doi:10.1002/cyto.990030402 · Cited by 139 · see citing works
Seeding Arterial Prostheses with Vascular Endothelium The Nature of the Lining
Herring, M. B., Dilley, R. S., Jersild, R. A., et al. (1979) · Annals of Surgery · doi:10.1097/00000658-197907000-00019 · Cited by 124 · see citing works
Expression of angiotensin‐converting enzyme activity in cultured pulmonary artery endothelial cells
Vecchio, P. J. D., & Smith, J. R. (1981) · Journal of Cellular Physiology · doi:10.1002/jcp.1041080307 · Cited by 123 · see citing works
Endothelial Cell Seeding of Prosthetic Vascular Grafts
Graham, L. M. (1980) · Archives of Surgery · doi:10.1001/archsurg.1980.01380080025005 · Cited by 109 · see citing works
Auerbach, R., Alby, L. J., Grieves, J., et al. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.24.7891 · Cited by 107 · see citing works
Craig, I. F., Via, D. P., Sherrill, B. C., et al. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68366-3 · Cited by 49 · see citing works
Self-citationIsolation and characterization of the murine macrophage acetyl LDL receptor
Via, D. P., Dresel, H. A., & Gotto, A. M. (1982) · Arteriosclerosis An Official Journal of the American Heart Association Inc · Cited by 7 · see citing works
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Transcytosis: Crossing Cellular Barriers
TUMA, P. L., & HUBBARD, A. L. (2003) · Physiological Reviews · doi:10.1152/physrev.00001.2003 · Cited by 681 · see citing works
Sayed, N., Huang, Y., Nguyen, K., Krejciova-Rajaniemi, Z., Grawe, A. P., Gao, T., Tibshirani, R., Hastie, T., Alpert, A., Cui, L., et al. (2021) · Nature Aging · doi:10.1038/s43587-021-00082-y · Cited by 572 · see citing works
Kennedy, M., D'Souza, S. L., Lynch-Kattman, M., Schwantz, S., & Keller, G. (2007) · Blood · doi:10.1182/blood-2006-09-047704 · Cited by 483 · see citing works
Batlle, R., Andrés, E., Gonzalez, L., Llonch, E., Igea, A., Gutierrez-Prat, N., Berenguer-Llergo, A., & Nebreda, A. R. (2019) · Nature Communications · doi:10.1038/s41467-019-10946-y · Cited by 142 · see citing works
Belair, D. G., Whisler, J. A., Valdez, J., Velazquez, J., Molenda, J. A., Vickerman, V., Lewis, R., Daigh, C., Hansen, T. D., Mann, D. A., et al. (2015) · Stem Cell Reviews and Reports · doi:10.1007/s12015-014-9549-5 · Cited by 120 · see citing works
Takeishi, K., Collin de l’Hortet, A., Wang, Y., Handa, K., Guzman-Lepe, J., Matsubara, K., Morita, K., Jang, S., Haep, N., Florentino, R. M., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107711 · Cited by 118 · see citing works
Narazaki, M., Segarra, M., & Tosato, G. (2008) · Blood · doi:10.1182/blood-2007-09-112474 · Cited by 59 · see citing works
Infection of central nervous system endothelial cells by cell-associated EHV-1
Goehring, L. S., Hussey, G. S., Ashton, L. V., Schenkel, A. R., & Lunn, D. P. (2011) · Veterinary Microbiology · doi:10.1016/j.vetmic.2010.08.030 · Cited by 34 · see citing works
Loeven, M. A., van Gemst, J. J., Schophuizen, C. M. S., Tilakaratna, V., van den Heuvel, L. P., Day, A. J., Klevering, B. J., & van der Vlag, J. (2018) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.iovs-17-22893 · Cited by 17 · see citing works
Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells
Van de Walle, G. R., Favoreel, H. W., Nauwynck, H. J., Mettenleiter, T. C., & Pensaert, M. B. (2003) · Journal of General Virology · doi:10.1099/vir.0.18796-0 · Cited by 15 · see citing works
The Therapeutic Potential of Hematopoietic Stem Cells in Bone Regeneration
Oliveira, C. S., Carreira, M., Correia, C. R., & Mano, J. F. (2022) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2021.0019 · Cited by 14 · see citing works
Boonpattrawong, N. P., Golbidi, S., Tai, D. C., Aleliunas, R. E., Bernatchez, P., Miller, J. W., Laher, I., & Devlin, A. M. (2020) · Physiological Reports · doi:10.14814/phy2.14582 · Cited by 14 · see citing works
- Authors
- Voyta, J. C., Via, D. P., Butterfield, C. E., & Zetter, B. R.
- Affiliations
- Boston Children's Hospital (US)
- ORCID
- Zetter, B R
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1984-12-01
- Volume
- 99
- Issue
- 6
- Pages
- 2034-2040
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2113570
- OpenAlex
- W2098400443
- Cited by
- 1,239
- References
- 27
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 9 · 2023: 4 · 2022: 6 · 2021: 15 · 2020: 17 · 2019: 17 · 2018: 15 · 2017: 19 · 2016: 17 · 2015: 22 · 2014: 17 · 2013: 21 · 2012: 20
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA037393)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- endothelial cells, acetylated low density lipoprotein, scavenger receptors, fluorescent cell labeling, fluorescence-activated cell sorting, microvascular endothelial cells, aortic endothelial cells, capillary endothelial cells, pericytes, smooth muscle cells, cell isolation, LDL metabolism
- MeSH terms
- Acetylation, Animals, Aorta, Cattle, Cell Separation, Cells, Cultured, Endothelium, Kinetics, Lipoproteins, LDL, Muscle, Smooth, Vascular, Receptors, LDL
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