Cited by 118Sphingolipid Metabolism and SignalingLipid metabolism and biosynthesisPlant biochemistry and biosynthesis

DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine

Open access
OMAE, Fumio, MIYAZAKI, Masao, ENOMOTO, Ayako, SUZUKI, Minoru, SUZUKI, Yusuke, SUZUKI, Akemi·2004·Biochemical Journal

Abstract

The C-4 hydroxylation of sphinganine and dihydroceramide is a rate-limiting reaction in the biosynthesis of phytosphingolipids. Mouse DES1 (MDES1) cDNA homologous to the Drosophila melanogaster degenerative spermatocyte gene-1 (des-1) cDNA leads to sphingosine Δ4-desaturase activity, and another mouse homologue, MDES2, has bifunctional activity, producing C-4 hydroxysphinganine and Δ4-sphingenine in yeast [Ternes, Franke, Zahringer, Sperling and Heinz (2002) J. Biol. Chem. 277, 25512–25518]. Here, we report the characterization of mouse DES2 (MDES2) using an in vitro assay with a homogenate of COS-7 cells transfected with MDES2 cDNA and N-octanoyl-sphinganine and sphinganine as substrates. MDES2 protein prefers dihydroceramide as a substrate to sphinganine, and exhibits dihydroceramide Δ4-desaturase and C-4 hydroxylase activities. MDES2 mRNA content was high in the small intestine and abundant in the kidney. In situ hybridization detected signals of MDES2 mRNA in the crypt cells. Immunohistochemistry using an anti-MDES2 peptide antibody stained the crypt cells and the adjacent epithelial cells. These results suggest that MDES2 is the dihydroceramide C-4 hydroxylase responsible for the biosynthesis of enriched phytosphingoglycolipids in the microvillous membranes of intestinal epithelial cells.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1042/bj20031425

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Sphingosine- and phytosphingosine-type ceramides are generated either via desaturation between the 4 and 5 positions of the LCB moiety of dihydroceramides (DS ceramides) by DEGS1, or via hydroxylation of the same moiety at the 4 position by DEGS2.

    “Sphingosine-and phytosphingosine-type ceramides are generated either via desaturation between the 4 and 5 positions of the LCB moiety of dihydroceramides (DS ceramides) by the desaturase DEGS1, or via hydroxylation of the same moiety at the 4 position by the hydroxylase DEGS2 (49,50).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 44 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
OMAE, F., MIYAZAKI, M., ENOMOTO, A., SUZUKI, M., SUZUKI, Y., & SUZUKI, A.
Affiliations
RIKEN (JP)
Venue
Biochemical Journal
Published
2004-05-01
Volume
379
Issue
3
Pages
687-695
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
OpenAlex
W2103244006
Cited by
118
References
44
Citations by year
2026: 2 · 2025: 4 · 2024: 4 · 2023: 11 · 2022: 8 · 2021: 6 · 2020: 4 · 2019: 2 · 2018: 5 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 3
FWCI
1.78
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Tokyo Medical and Dental University
  • RIKEN
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
small intestine, intestinal epithelial cells, crypt cells, sphinganine, dihydroceramide
MeSH terms
Animals, Cell Extracts, Ceramides, Mixed Function Oxygenases, Immunohistochemistry, Intestine, Small, Membrane Proteins, Multienzyme Complexes, Organ Specificity, Oxidoreductases, RNA, Messenger, Sphingosine, Transfection, In Situ Hybridization, COS Cells, Fatty Acid Desaturases, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review