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Cited by 647

The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.

Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization

Open access
LAULAGNIER, Karine, MOTTA, Claude, HAMDI, Safouane, ROY, Sébastien, FAUVELLE, Florence, PAGEAUX, Jean-François, KOBAYASHI, Toshihide, SALLES, Jean-Pierre, PERRET, Bertrand, BONNEROT, Christian, RECORD, Michel·2004·Biochemical Journal
DOI 10.1042/bj20031594
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Abstract

Exosomes are small vesicles secreted from multivesicular bodies, which are able to stimulate the immune system leading to tumour cell eradication. We have analysed lipids of exosomes secreted either upon stimulation from rat mast cells (RBL-2H3 cells), or constitutively from human dendritic cells. As compared with parent cells, exosomes displayed an enrichment in sphingomyelin, but not in cholesterol. Phosphatidylcholine content was decreased, but an enrichment was noted in disaturated molecular species as in phosphatidylethanolamines. Lyso(bis)phosphatidic acid was not enriched in exosomes as compared with cells. Fluorescence anisotropy demonstrated an increase in exosome-membrane rigidity from pH 5 to 7, suggesting their membrane reorganization between the acidic multivesicular body compartment and the neutral outer cell medium. NMR analysis established a bilayer organization of exosome membrane, and ESR studies using 16-doxyl stearic acid demonstrated a higher flip-flop of lipids between the two leaflets as compared with plasma membrane. In addition, the exosome membrane exhibited no asymmetrical distribution of phosphatidylethanolamines. Therefore exosome membrane displays a similar content of the major phospholipids and cholesterol, and is organized as a lipid bilayer with a random distribution of phosphatidylethanolamines. In addition, we observed tight lipid packing at neutral pH and a rapid flip-flop between the two leaflets of exosome membranes. These parameters could be used as a hallmark of exosomes.

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Crossref · 10.1042/bj20031594

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Metadata

Authors
LAULAGNIER, K., MOTTA, C., HAMDI, S., ROY, S., FAUVELLE, F., PAGEAUX, J.-F., KOBAYASHI, T., SALLES, J.-P., PERRET, B., BONNEROT, C., & RECORD, M.
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
2004-05-15
Volume
380
Issue
1
Pages
161-171
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
DOI
10.1042/bj20031594
OpenAlex
W2126400039
Cited by
647
References
49
FWCI
2.84
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization. https://app.cochat.ai/papers/10.1042/bj20031594
The paper
LAULAGNIER, K., MOTTA, C., HAMDI, S., ROY, S., FAUVELLE, F., PAGEAUX, J.-F., KOBAYASHI, T., SALLES, J.-P., PERRET, B., BONNEROT, C., & RECORD, M. (2004). Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization. Biochemical Journal. https://doi.org/10.1042/bj20031594

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.