Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2
Open accessAbstract
To elucidate the specific biological role of the yeast homologues of PTPA (phosphatase 2A phosphatase activator), Ypa1 and Ypa2 (where Ypa stands for yeast phosphatase activator), in the regulation of PP2A (protein phosphatase 2A), we investigated the physical interaction of both Ypa proteins with the catalytic subunit of the different yeast PP2A-like phosphatases. Ypa1 interacts specifically with Pph3, Sit4 and Ppg1, whereas Ypa2 binds to Pph21 and Pph22. The Ypa1 and Ypa2 proteins do not compete with Tap42 (PP2A associating protein) for binding to PP2A family members. The interaction of the Ypa proteins with the catalytic subunit of PP2A-like phosphatases is direct and independent of other regulatory subunits, implicating a specific function for the different PP2A-Ypa complexes. Strikingly, the interaction of Ypa2 with yeast PP2A is promoted by the presence of Ypa1, suggesting a positive role of Ypa1 in the regulation of PP2A association with other interacting proteins. As in the mammalian system, all yeast PP2A-like enzymes associate as an inactive complex with Yme (yeast methyl esterase). Ypa1 as well as Ypa2 can reactivate all these inactive complexes, except Pph22-Yme. Ypa1 is the most potent activator of PP2A activity, suggesting that there is no direct correlation between activation potential and binding capacity.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Caution is needed when interpreting biogenesis regulator strains because several of these proteins also affect PP4 and PP6, so their knockout or overexpression may not affect only PP2A.
“Some caution in interpreting data for the PP2A biogenesis regulator strains is however warranted, as several of these proteins, besides PP2A, likely also affect the functions of the PP2A-like phosphatases PP4 and PP6 [23][24][25][26], and hence, their knockout or overexpression may not solely affect PP2A.”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [23]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A comprehensive two-hybrid analysis to explore the yeast protein interactome
Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., & Sakaki, Y. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.061034498 · Cited by 3,566 · see citing works
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
James, P. M., Halladay, J. T., & Craig, E. A. (1996) · Genetics · doi:10.1093/genetics/144.4.1425 · Cited by 2,638 · see citing works
Janssens, V., & Goris, J. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3530417 · Cited by 1,890 · see citing works
Self-citationJanssens, V., & Goris, J. (2001) · Biochemical Journal · doi:10.1042/bj3530417 · Cited by 1,392 · see citing works
Self-citationTOR signalling in bugs, brain and brawn
Jacinto, E., & Hall, M. N. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1018 · Cited by 560 · see citing works
Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases.Preprint
Como, C. J. D., & Arndt, K. T. (1996) · Genes & Development · doi:10.1101/gad.10.15.1904 · Cited by 494 · see citing works
The SIT4 protein phosphatase functions in late G1 for progression into S phase.
Sutton, A., Immanuel, D., & Arndt, K. T. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.4.2133 · Cited by 337 · see citing works
TIP41 Interacts with TAP42 and Negatively Regulates the TOR Signaling Pathway
Jacinto, E., Guo, B., Arndt, K. T., Schmelzle, T., & Hall, M. N. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00386-0 · Cited by 228 · see citing works
Murata, K., Wu, J., & Brautigan, D. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.20.10624 · Cited by 213 · see citing works
α4 Associates with Protein Phosphatases 2A, 4, and 6
Chen, J., Peterson, R. T., & Schreiber, S. L. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.8792 · Cited by 175 · see citing works
BRYANT, J. C., Westphal, R. S., & Wadzinski, B. E. (1999) · Biochemical Journal · doi:10.1042/bj3390241 · Cited by 173 · see citing works
The SAPs, a New Family of Proteins, Associate and Function Positively with the SIT4 Phosphatase
Luke, M. M., Seta, F. D., Como, C. J. D., et al. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.6.2744 · Cited by 166 · see citing works
Multiple Roles of Tap42 in Mediating Rapamycin-Induced Transcriptional Changes in Yeast
Düvel, K., Santhanam, A. N., Garrett, S., Schneper, L. M., & Broach, J. R. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00228-4 · Cited by 164 · see citing works
Wei, H., Ashby, D. G., Moreno, C. S., Ogris, E., Yeong, F. M., Corbett, A. H., & Pallas, D. C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008694200 · Cited by 128 · see citing works
Nanahoshi, M., Nishiuma, T., Tsujishita, Y., Hara, K., Inui, S., Sakaguchi, N., & Yonezawa, K. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9493 · Cited by 114 · see citing works
The SIT4 Protein Phosphatase Functions in Late G 3 for Progression into S Phase
Sutton, A., Immanuel, D., & Arndt, K. T. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.4.2133-2148.1991 · Cited by 109 · see citing works
Fellner, T., Lackner, D. H., Hombauer, H., Piribauer, P., Mudrak, I., Zaragoza, K., Juno, C., & Ogris, E. (2003) · Genes & Development · doi:10.1101/gad.259903 · Cited by 105 · see citing works
Longin, S., Jordens, J., MARTENS, E., Stevens, I., Janssens, V., Rondelez, E., BAERE, I. D., Derua, R., Waelkens, E., Goris, J., et al. (2004) · Biochemical Journal · doi:10.1042/bj20031643 · Cited by 101 · see citing works
Self-citationEvans, D. R., & Stark, M. J. R. (1997) · Genetics · doi:10.1093/genetics/145.2.227 · Cited by 94 · see citing works
Cayla, X., Goris, J., Hermann, J., Hendrix, P., Ozon, R., & Merlevede, W. (1990) · Biochemistry · doi:10.1021/bi00455a010 · Cited by 91 · see citing works
Self-citationInteraction with Tap42 Is Required for the Essential Function of Sit4 and Type 2A Phosphatases
Wang, H., Wang, X., & Jiang, Y. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-02-0072 · Cited by 87 · see citing works
Li, X., & Virshup, D. M. (2002) · European Journal of Biochemistry · doi:10.1046/j.0014-2956.2001.02680.x · Cited by 83 · see citing works
Cayla, X., Hoof, C. A. V., Bosch, M., Waelkens, E., Vandekerckhove, J. S., Peeters, B., Merlevede, W. J., & Goris, J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40733-2 · Cited by 73 · see citing works
Self-citationKloeker, S., Reed, R. B., McConnell, J. L., Chang, D., Tran, K. C., Westphal, R. S., Law, B. K., Colbran, R. J., Kamoun, M., Campbell, K. S., et al. (2003) · Protein Expression and Purification · doi:10.1016/s1046-5928(03)00141-4 · Cited by 67 · see citing works
Rempoła, B., Kaniak, A., Migdalski, A., Rytka, J., Słonimski, P. P., & di Rago, J. (2000) · Molecular and General Genetics MGG · doi:10.1007/pl00008651 · Cited by 62 · see citing works
Protein phosphatase 2A on track for nutrient‐induced signalling in yeast
Zabrocki, P., HOOF, C. V., Goris, J., Thevelein, J. M., Winderickx, J., & Wera, S. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02786.x · Cited by 58 · see citing works
Self-citationThe gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation.
Posas, F., Clotet, J., Muns, M. T., Corominas, J., Casamayor, A., & Ariño, J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54082-5 · Cited by 57 · see citing works
Mitchell, D. A., & Sprague, G. F. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.2.488-500.2001 · Cited by 44 · see citing works
Wang, H., & Jiang, Y. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.9.3116-3125.2003 · Cited by 31 · see citing works
HOOF, C. V., Janssens, V., BAERE, I. D., Stark, M. J. R., de Winde, J. H., Winderickx, J., Thevelein, J. M., Merlevede, W. J., & Goris, J. (2001) · Experimental Cell Research · doi:10.1006/excr.2000.5144 · Cited by 28 · see citing works
Self-citationHOOF, C. V., Janssens, V., BAERE, I. D., et al. (2000) · Journal of Molecular Biology · doi:10.1006/jmbi.2000.4062 · Cited by 27 · see citing works
Self-citationHOOF, C. V., Janssens, V., Dinishiotu, A., et al. (1998) · Biochemistry · doi:10.1021/bi980496l · Cited by 24 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Sents, W., Ivanova, E., Lambrecht, C., Haesen, D., & Janssens, V. (2013) · The FEBS Journal · doi:10.1111/j.1742-4658.2012.08579.x · Cited by 204 · see citing works
Self-citationPhysiologic functions of PP2A: Lessons from genetically modified mice
Reynhout, S., & Janssens, V. (2019) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2018.07.010 · Cited by 124 · see citing works
Self-citationMcConnell, J. L., Gomez, R. J., McCorvey, L. R. A., Law, B. K., & Wadzinski, B. E. (2007) · Oncogene · doi:10.1038/sj.onc.1210406 · Cited by 80 · see citing works
- Authors
- Van HOOF, C., MARTENS, E., LONGIN, S., JORDENS, J., STEVENS, I., JANSSENS, V., & GORIS, J.
- Affiliations
- Afdeling Biochemie, Faculteit Geneeskunde, Campus Gasthuisberg O&N, Katholieke Universiteit Leuven, Herestraat 49, B-3000 Leuven, Belgium
- KU Leuven (BE)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2005-02-08
- Volume
- 386
- Issue
- 1
- Pages
- 93-102
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1134770
- OpenAlex
- W2032966848
- Cited by
- 42
- References
- 32
- Citations by year
- 2023: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Lirias (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- protein phosphatase 2A, PTPA, protein-protein interaction, triphenylphosphine, Sit4, phosphatase activation, protein phosphatase regulation, Saccharomyces cerevisiae
- MeSH terms
- Carboxylic Ester Hydrolases, Enzyme Activation, Phosphoprotein Phosphatases, Phosphorylation, Protein Binding, Protein Processing, Post-Translational, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Structure-Activity Relationship, Peptidylprolyl Isomerase, Sirolimus, Catalytic Domain, Two-Hybrid System Techniques, Protein Subunits, Protein Interaction Mapping, Saccharomyces cerevisiae Proteins, Multiprotein Complexes, Intracellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Protein Phosphatase 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
