The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Assessing mitochondrial dysfunction in cells
Open accessAbstract
Assessing mitochondrial dysfunction requires definition of the dysfunction to be investigated. Usually, it is the ability of the mitochondria to make ATP appropriately in response to energy demands. Where other functions are of interest, tailored solutions are required. Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance. There are many methods to measure mitochondrial function and dysfunction in these systems. Generally, measurements of fluxes give more information about the ability to make ATP than do measurements of intermediates and potentials. For isolated mitochondria, the best assay is mitochondrial respiratory control: the increase in respiration rate in response to ADP. For intact cells, the best assay is the equivalent measurement of cell respiratory control, which reports the rate of ATP production, the proton leak rate, the coupling efficiency, the maximum respiratory rate, the respiratory control ratio and the spare respiratory capacity. Measurements of membrane potential provide useful additional information. Measurement of both respiration and potential during appropriate titrations enables the identification of the primary sites of effectors and the distribution of control, allowing deeper quantitative analyses. Many other measurements in current use can be more problematic, as discussed in the present review.
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Cites
Fission and selective fusion govern mitochondrial segregation and elimination by autophagy
Twig, G., Elorza, A., Molina, A. J. A., Mohamed, H., Wikstrom, J. D., Walzer, G., Stiles, L., Haigh, S. E., Katz, S., Las, G., et al. (2008) · The EMBO Journal · doi:10.1038/sj.emboj.7601963 · Cited by 3,082
RESPIRATORY ENZYMES IN OXIDATIVE PHOSPHORYLATION
Chance, B., & Williams, G. R. (1955) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)57189-7 · Cited by 1,473
The causes and functions of mitochondrial proton leak
Brand, M. D., Chien, L.-F., Ainscow, E. K., Rolfe, D. F. S., & Porter, R. K. (1994) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(94)90099-x · Cited by 466
Self-citationThe efficiency and plasticity of mitochondrial energy transduction.
Brand, M. D. (2005) · Biochemical Society Transactions · doi:10.1042/bst0330897 · Cited by 406
Self-citationYadava, N., & Nicholls, D. G. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.0212-07.2007 · Cited by 272
Self-citationMitochondrial dysfunction and oxidative stress: A contributing link to acquired epilepsy?
Waldbaum, S., & Patel, M. (2010) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-010-9320-9 · Cited by 264
Cadenas, S., Echtay, K. S., Harper, J. A., Jekabsons, M. B., Buckingham, J. A., Grau, E., Abuin, A., Chapman, H., Clapham, J. C., & Brand, M. D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109736200 · Cited by 199
Self-citationAffourtit, C., & Brand, M. D. (2008) · Biochemical Journal · doi:10.1042/bj20070954 · Cited by 85
Self-citationRocha, M., Apostolova, N., Hernandez-Mijares, A., Herance, R., & M. Victor, V. (2010) · Current Medicinal Chemistry · doi:10.2174/092986710793205444 · Cited by 75
Stronger control of ATP/ADP by proton leak in pancreatic β-cells than skeletal muscle mitochondria
Affourtit, C., & Brand, M. D. (2006) · Biochemical Journal · doi:10.1042/bj20051280 · Cited by 71
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Cited by
Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells
Sullivan, L. B., Gui, D. Y., Hosios, A. M., Bush, L. N., Freinkman, E., & Vander Heiden, M. G. (2015) · Cell · doi:10.1016/j.cell.2015.07.017 · Cited by 1,173
Everts, B., Amiel, E., Huang, S. C.-C., Smith, A. M., Chang, C.-H., Lam, W. Y., Redmann, V., Freitas, T. C., Blagih, J., van der Windt, G. J. W., et al. (2014) · Nature Immunology · doi:10.1038/ni.2833 · Cited by 1,064
Mitochondrial dysfunction in cell senescence and aging
Miwa, S., Kashyap, S., Chini, E., & von Zglinicki, T. (2022) · Journal of Clinical Investigation · doi:10.1172/jci158447 · Cited by 1,061
Brand, M. D. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.04.001 · Cited by 1,063
Self-citationMitochondrial dynamics regulates migration and invasion of breast cancer cells
Zhao, J., Zhang, J., Yu, M., Xie, Y., Huang, Y., Wolff, D. W., Abel, P. W., & Tu, Y. (2013) · Oncogene · doi:10.1038/onc.2012.494 · Cited by 759
Metadata
- Authors
- Brand, M. D., & Nicholls, D. G.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2011-03-29
- Volume
- 435
- Issue
- 2
- Pages
- 297-312
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2143505819
- Cited by
- 2,405
- References
- 10
- FWCI
- 40.20
- Type
- journal article
- Language
- en
- Open access
- hybrid
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